European Journal of Forest Research

, Volume 133, Issue 1, pp 131–139 | Cite as

The effect of thinning on microbial biomass C, N and basal respiration in black pine forest soils in Mudurnu, Turkey

  • İlyas BolatEmail author
Original Paper


Reducing the canopy cover (e.g., forest thinning) is one of the most commonly employed forest silvicultural treatments. Trees are partially removed from a forest in order to manage tree competition, thus favoring the remaining and often the most valuable trees. The properties of the soil are affected by forest thinning as a result of changes in key microclimatic conditions, microbial communities and biomass, root density, nutrient budgets and organic matter turnover. The aim of this study was to determine the soil microbial biomass C, N and respiration (basal respiration) in a black pine (Pinus nigra Arn. subsp. pallasiana) forest in the Mudurnu district of Bolu Province (Western Black Sea Region, Turkey). Whereas forest thinning was found to cause increases in the soil temperature, microbial biomass C and N and organic C, it was found to decrease the soil moisture, basal respiration and metabolic quotient (qCO2). As expected, soil organic C exhibited a strong impact on soil microbial biomass C, N and basal respiration. It was concluded that the influence of forest thinning on the microbial biomass and soil respiration was the combined result of changing microclimatic conditions and soil properties, such as forest litter, soil temperature, soil moisture, soil pH and soil organic matter.


Basal respiration Black pine Metabolic quotient (qCO2Microbial biomass Thinning 



I am grateful to Assistant Professor Hüseyin ŞENSOY, Research Assistant Kamil ÇAKIROĞLU and Chief of Forest Management Yusuf ADIGÜZEL for their valuable efforts during the field and laboratory works. The Turkish General Directorate of Forestry (TGDF) and Turkish State Meteorological Service (TSMS) are also gratefully acknowledged for their data support throughout this study.


  1. Alef K (1995) Soil respiration. In: Alef K, Nannipieri P (eds) Methods in applied soil microbiology and biochemistry. Academic Press, London, pp 214–218Google Scholar
  2. Alvarez R, Santanatoglia OJ, Garcia R (1995) Effect of temperature on soil microbial biomass and its metabolic quotient in situ under different tillage systems. Biol Fertil Soils 19:227–230CrossRefGoogle Scholar
  3. Alvarez E, Torrado VM, Fernandez Marcos ML, Diaz-Ravia M (2009) Microbial biomass and activity in a forest soil under different tree species. Electron J Environ Agric Food Chem 8(9):878–887Google Scholar
  4. Anderson TH (2003) Microbial eco-physiological indicators to assess soil quality. Agric Ecosyst Environ 98:285–293CrossRefGoogle Scholar
  5. Anderson TH, Domsch KH (1985) Maintenance requirements of actively metabolizing microbial populations under in situ conditions. Soil Biol Biochem 17:197–203CrossRefGoogle Scholar
  6. Anderson TH, Domsch KH (1986) Carbon link between microbial biomass and soil organic matter. In: Megusar F, Gantar M (eds) Perspectives in microbial ecology. Slovene Society for Microbi-ology, Ljubljana, pp 467–471Google Scholar
  7. Anderson JPE, Domsch KH (1989) Ratios of microbial biomass carbon to total organic carbon in arable soils. Soil Biol Biochem 21:471–479CrossRefGoogle Scholar
  8. Anderson TH, Domsch KH (1990) Application of eco-physiological quotients (qCO2, and qD) on microbial biomasses from soils of different cropping histories. Soil Biol Biochem 22:251–255CrossRefGoogle Scholar
  9. Anderson TH, Domsch KH (1993) The metabolic quotient for CO2 (qCO2) as a specific activity parameter to assess the effects of environmental conditions, such as pH, on the microbial biomass of forest soils. Soil Biol Biochem 25:393–395CrossRefGoogle Scholar
  10. Anderson JM, Ingram JSI (1996) Tropical soil biology and fertility a handbook of methods, 2nd edn. Cab International Wallingford, UKGoogle Scholar
  11. Balota EL, Colozzi-Filho A, Andrade DS, Dick RP (2003) Microbial biomass in soils under different tillage and crop rotation systems. Biol Fertil Soil 38:15–20CrossRefGoogle Scholar
  12. Bauhus J, Khanna PK (1999) The significance of microbial biomass in forest soils. In: Rastin N, Bauhus J (eds) Going underground-ecological studies in forest soils. Research Signpost, Trivandrum, pp 77–110Google Scholar
  13. Bauhus JD, Pare D, Cote L (1998) Effects of tree species, stand age, and soil type on soil microbial biomass and its activity in a southern boreal forest. Soil Biol Biochem 30:1077–1089CrossRefGoogle Scholar
  14. Blake GR, Hartge KH (1986) Bulk density. In: Klute A (ed) Methods of Soil Analysis, Part 1. Physical and Mineralogical Methods, Agronomy Monograph 9, American Society of Agronomy-Soil Science Society of America, Madison, pp 363–375Google Scholar
  15. Boerner REJ, Waldrop TA, Shelburne VB (2006) Wildfire mitigation strategies affect soil enzyme activity and soil organic carbon in loblolly pine (Pinus taeda) forests. Can J For Res 36(12):3148–3154CrossRefGoogle Scholar
  16. Bouyoucos GJ (1962) Hydrometer method improved for making particle size analyses of soils. Agron J 54:464–465CrossRefGoogle Scholar
  17. Brady NC (1990) The nature and properties of soils, 10th edn. Macmillan, New YorkGoogle Scholar
  18. Brookes PC (1995) The use of microbial parameters in monitoring soil pollution by heavy metals. Biol Fertil Soils 19:269–279CrossRefGoogle Scholar
  19. Brookes PC, Landman A, Pruden G, Jenkinson DS (1985) Chloroform fumigation and the release of soil nitrogen: a rapid extraction method to measure microbial biomass nitrogen in soil. Soil Biol Biochem 17:837–842CrossRefGoogle Scholar
  20. Chen CR, Condron LM, Davis MR, Sherlock RR (2003) Seasonal changes in soil phosphorus and associated microbial properties under adjacent grassland and forest in New Zealand. For Ecol Manage 177:539–557CrossRefGoogle Scholar
  21. Davidson EA, Belk E, Boone RD (1998) Soil water content and temperature as independent or confounded factors controlling soil respiration in a temperate mixed hardwood forest. Glob Change Biol 4:217–227CrossRefGoogle Scholar
  22. Devi NB, Yadava PS (2006) Seasonal dynamics in soil microbial biomass C, N and P in a mixed-oak forest ecosystem of Manipur, Northeast India. Appl Soil Ecol 31:220–227CrossRefGoogle Scholar
  23. Diaz-Ravina M, Caraballas T, Acea MJ (1988) Microbial biomass and activity in four acid soils. Soil Biol Biochem 20:817–823CrossRefGoogle Scholar
  24. Dilly O, Munch JC (1998) Ratios between estimates of microbial biomass content and microbial activity in soils. Biol Fertil Soils 27:374–379CrossRefGoogle Scholar
  25. Dilly O, Bernhard M, Kutsch WL, Kappen L, Munch JC (1997) Aspects of carbon and nitrogen cycling in soils of the Bornhoved Lake district I. Microbial characteristics and emission of carbon dioxide and nitrous oxide of arable and grassland soils. Biogeochemistry 39:189–205CrossRefGoogle Scholar
  26. Dilustro JJ, Collins B, Duncan L, Crawford C (2005) Moisture and soil texture effects on soil CO2 efflux components in southeastern mixed pine forests. For Ecol Manage 204:85–95CrossRefGoogle Scholar
  27. Franzluebbers AJ, Haney RL, Hons FM (1999) Relationships of chloroform fumigation–incubation to soil organic matter pools. Soil Biol Biochem 31:395–405CrossRefGoogle Scholar
  28. Giai C, Boerner REJ (2007) Effects of ecological restoration on microbial activity, microbial functional diversity, and soil organic matter in mixed-oak forests of southern Ohio, USA. Appl Soil Ecol 35:281–290CrossRefGoogle Scholar
  29. Grady KC, Hart SC (2006) Influences of thinning, prescribed burning, and wildfire on soil processes and properties in southwestern ponderosa pine stands: a retrospective study. For Ecol Manage 234:123–135CrossRefGoogle Scholar
  30. Hernot J, Robertson GP (1994) Vegetation removal in two soils of the humid tropics: effect on microbial biomass. Soil Biol Biochem 26:111–116CrossRefGoogle Scholar
  31. Insam H (2001) Developments in soil microbiology since the mid 1960s. Geoderma 100:389–402CrossRefGoogle Scholar
  32. Insam H, Haselwandter K (1989) Metabolic quotient of the soil microflora in relation to plant succession. Oecologia 79:174–178CrossRefGoogle Scholar
  33. Insam H, Hutchinson TC, Reber HH (1996) Effects of heavy metal stress on the metabolic quotient of soil microflora. Soil Biol Biochem 28:691–694CrossRefGoogle Scholar
  34. Jassal RS, Black TA, Cai T, Morgenstern K, Li Z, Gaumont-Guay D, Nesic Z (2007) Components of ecosystem respiration and an estimate of net primary productivity of an intermediate-aged Douglas-fir stand. Agric For Meteorol 144:44–57CrossRefGoogle Scholar
  35. Jenkinson DS (1988) The determination of microbial biomass carbon and nitrogen in soil. In: Wilson JR (ed) Advances in nitrogen cycling in agricultural ecosystems. CAB, Wallingford, pp 368–386Google Scholar
  36. Jenkinson DS, Ladd JN (1981) Microbial biomass in soil measurement and turnover. In: Paul EA, Ladd JN (eds) Soil biochemistry, vol 5. Marcel Dekker Inc, New York and Basel, pp 415–471Google Scholar
  37. Joergensen RG, Anderson TH, Wolters V (1995) Carbon and nitrogen relationships in the microbial biomass of soils in beech (Fagus sylvatica L.) forests. Biol Fertil Soils 19:141–147CrossRefGoogle Scholar
  38. Johnson DW, Curtis PS (2001) Effects of forest management on soil C and N storage: meta-analysis. For Ecol Manage 140:227–238CrossRefGoogle Scholar
  39. Kaiser EA, Muller T, Roergensen RG, Insam H, Heinemeyer O (1992) Evaluation of methods to estimate the soil microbial biomass and the relationship with soil texture and organic matter. Soil Biol Biochem 24:675–683CrossRefGoogle Scholar
  40. Kara Ö, Bolat İ (2007) The effect of wildfire on the microbial biomass C of black pine plantation soils. In: Proceedings of international symposium, bottlenecks, solutions, and priorities in the context of functions of forest resources, October 17–19, Istanbul University, Faculty of Forestry, Istanbul, Turkey, pp 1021–1030Google Scholar
  41. Kara Ö, Bolat İ (2008a) The effect of different land uses on soil microbial biomass carbon and nitrogen in Bartın province. Turk J Agric For 32(4):281–288Google Scholar
  42. Kara Ö, Bolat İ (2008b) Soil microbial biomass C and N changes in relation to forest conversion in the northwestern Turkey. Land Degrad Dev 19:421–428CrossRefGoogle Scholar
  43. Kara Ö, Bolat İ (2008c) Microbial biomass C (Cmic) and N (Nmic) content of forest and agricultural soils in Bartın province, Turkey. Ecology 18(69):32–40Google Scholar
  44. Kara Ö, Bolat İ, Çakıroğlu K, Öztürk M (2008) Plant canopy effects on litter accumulation and soil microbial biomass in two temperate forests. Biol Fertil Soils 45(2):193–198CrossRefGoogle Scholar
  45. Kara Ö, Şensoy H, Bolat İ (2010) Slope length effects on microbial biomass and activity of eroded sediments. J Soil Sediment 10(3):434–439CrossRefGoogle Scholar
  46. Khan KS, Joergensen RG (2006) Microbial C, N and P relationships in moisture stressed soils of Potohar, Pakistan. J Plant Nutr Soil Sci 169:494–500CrossRefGoogle Scholar
  47. Koçyiğit R (2008) The effect of soil management systems on microbial activity. International meeting on soil fertility land management and agro climatology, Turkey, pp 899–907Google Scholar
  48. Ma S, Chen J, North M, Erikson H, Bresee M, LeMoine J (2004) Short-term effects of experimental burning and thinning on soil respiration in an old- growth, mixed conifer forest. Environ Manage 33(Supplement 1):148–159Google Scholar
  49. Mahia J, Perez Ventura L, Cabaneiro A, Diaz-Ravina M (2006) Soil microbial biomass under pine forest in the north-wester Spain: influence of stand age, site index and parent material. Inv Agrar-Sist Rec F 15(2):152–159Google Scholar
  50. Martikainen PJ, Palojarvi A (1990) Evaluation of the fumigation-extraction method for the determination of microbial C and N in a range of forest soils. Soil Biol Biochem 22:797–802CrossRefGoogle Scholar
  51. Masyagina OV, Prokushkin SG, Koike T (2010) The influence of thinning on the ecological conditions and soil respiration in a Larch forest on Hokkaido Island. Eurasian Soil Sci 43:693–700CrossRefGoogle Scholar
  52. Merino A, Fernandez-Lopez A, Solla-Gullon F, Edeso JM (2004) Soil changes and tree growth in intensively managed Pinus radiata in northern Spain. For Ecol Manage 196:393–404CrossRefGoogle Scholar
  53. Nilsen P, Strand LT (2008) Thinning intensity effects on carbon and nitrogen stores and fluxes in a Norway spruce (Picea abies (L.) Karst.) stand after 33 years. For Ecol Manage 256:201–208CrossRefGoogle Scholar
  54. Odum EP (1985) Trends expected in stressed ecosystems. Bioscience 35:419–422CrossRefGoogle Scholar
  55. Olajuyigbe S, Tobin B, Gardiner P, Nieuwenhuis M (2012) Forest thinning and soil respiration in a managed Sitka spruce forest in Ireland. Agric For Meteorol 157:86–95CrossRefGoogle Scholar
  56. Patel K, Nirmal Kumar JIN, Kumar R, Kumar Bhoi R (2010) Seasonal and temporal variation in soil microbial biomass C, N and P in different types land uses of dry deciduous forest ecosystem of Udaipur, Rajasthan, Western India. Appl Ecol Environ Res 8(4):377–390Google Scholar
  57. Powlson DS, Jenkinson DS (1981) A comparison of the organic-matter, biomass, adenosine-triphosphate and mineralizable nitrogen contents of ploughed and direct-drilled soils. J Agric Sci 97:713–721CrossRefGoogle Scholar
  58. Powlson DS, Brookes PC, Christensen BT (1987) Measurement of microbial biomass provides an early indication of changes in total soil organic matter due to the straw incorporation. Soil Biol Biochem 19:159–164CrossRefGoogle Scholar
  59. Qiu S, Bell RW, Hobbs RJ, Mccomb AJ (2012) Estimating nutrient budgets for prescribed thinning in a regrowth eucalyptus forest in south-west Australia. Forestry 85(1):51–61CrossRefGoogle Scholar
  60. Rowell DL (1994) Soil science; methods and applications. Longman Publishers (Pte) Ltd, SingaporeGoogle Scholar
  61. Saffigna PG, Powlson DS, Brookes PC, Thomas GA (1989) Influence of sorghum residues and tillage on soil organic matter and soil microbial biomass in an Australian Vertisol. Soil Biol Biochem 21:759–765CrossRefGoogle Scholar
  62. Sparling GP (1997) Soil microbial biomass, activity and nutrient cycling as indicators of soil health. In: Pankhurst CE, Doube BM, Gupta VVSR (eds) Biological indicators of soil health. CAB International, Wallingford, pp 97–119Google Scholar
  63. Tang J, Qi Y, Xu M, Misson L, Goldstein AH (2005) Forest thinning and soil respiration in a ponderosa pine plantation in the Sierra Nevada. Tree Physiol 25:57–66PubMedCrossRefGoogle Scholar
  64. TGDF (Turkish General Directorate of Forestry) (2010) Forest management plans of Mudurnu forest administration. Ankara, Turkey Google Scholar
  65. Thornthwaite CW (1948) An approach toward a rational classification of climate. Geogr Rev 38:55–94CrossRefGoogle Scholar
  66. Tian Y, Haibara K, Toda H, Ding F, Liu Y, Choi D (2008) Microbial biomass and activity along a natural pH gradient in forest soils in a karst region of the upper Yangtze River, China. J For Res Jpn 13:205–214CrossRefGoogle Scholar
  67. TSMS (Turkish State Meteorological Service) (2010) Daily meteorological data. Ankara, TurkeyGoogle Scholar
  68. Vance ED, Brookes PC, Jenkinson DS (1987a) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19:703–707CrossRefGoogle Scholar
  69. Vance ED, Brookes PC, Jenkinson DS (1987b) Microbial biomass measurements in forest soils: the use of chloroform fumigation-incubations methods for strongly acid soils. Soil Biol Biochem 19:697–702CrossRefGoogle Scholar
  70. Vesala T, Suni T, Rannik Ü et al (2005) Effect of thinning on surface fluxes in a boreal forest. Global Biogeochem Cycles 19:GB2001. doi: 10.1029/2004GB002316 CrossRefGoogle Scholar
  71. Vesterdal L, Dalsgaard M, Felby C, Raulund-Rasmussen K, Jorgensen BB (1995) Effects of thinning and soil properties on accumulation of carbon, nitrogen and phosphorus in the forest floor of Norway spruce stands. For Ecol Manage 77:1–10CrossRefGoogle Scholar
  72. Wardle DA (1992) A comparative assessment of factors which influence microbial biomass carbon and nitrogen in soil. Biol Rev 67:321–358CrossRefGoogle Scholar
  73. Weiskittel AR, Hann DW, JrJA Kershaw, Vanclay JK (2011) Forest growth and yield modeling. Wiley-Blackwell, UKCrossRefGoogle Scholar
  74. Winding A, Hund-Rinke K, Rutgers M (2005) The use of microorganisms in ecological soil classification and assessment concepts. Ecotoxicol Environ Saf 62:230–248PubMedCrossRefGoogle Scholar
  75. Wu J, He Z-L, Wei W-X, O’Donnell AG, Syers JK (2000) Quantifying microbial biomass phosphorus in acid soils. Biol Fertil Soils 32:500–507CrossRefGoogle Scholar
  76. Zeller B, Colin-Belgrand M, Dambrine E, Martin F, Bottner P (2000) Decomposition of 15 N-labelled beech litter and fate of nitrogen derived from litter in a beech forest. Oecologia 123:550–559CrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Division of Soil Science and Ecology, Department of Forest Engineering, Faculty of ForestryBartın UniversityBartınTurkey

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