European Journal of Forest Research

, Volume 128, Issue 5, pp 431–436 | Cite as

Plastid DNA variation in Prunus serotina var. serotina (Rosaceae), a North American tree invading Europe

  • B. Petitpierre
  • M. Pairon
  • O. Broennimann
  • A. L. Jacquemart
  • A. Guisan
  • Guillaume Besnard
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

Black cherry (Prunus serotina) is a tree from North America, where it is often used for economical purposes, whereas it is widespread and invasive in Europe. Plastid DNA variation was first investigated in both its native and invasive ranges using microsatellite loci and sequences of three intergenic spacers (trnT-trnL, trnD-trnT and trnS-trnG). This analysis was focused on P. serotina var. serotina, with the inclusion of samples of closely related taxa. Length variation at a microsatellite locus (ccmp5) and a few sequence polymorphisms were identified among P. serotina samples. Four new primer pairs were then designed to specifically amplify variable regions and a combination of five markers was finally proposed for phylogeographic studies in P. serotina. These loci allow identification of six chlorotypes in P. serotina var. serotina, which may be particularly useful to depict the maternal origins of European invasive populations.

Keywords

Microsatellite PCR-RFLP Plastid DNA Population genetics 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

This research was funded by the National Centre of Competence in Research (NCCR) Plant Survival, research program of the Swiss National Science Foundation. M. Pairon and A.L. Jacquemart were supported by the Fonds Spéciaux de Recherche (FSR) of the Université catholique de Louvain (UCL) and the Belgian Scientific Policy (BelSPo-InPlanBel). M. Pairon is research fellow of the Belgian National Fund of Scientific Research and A.L. Jacquemart is research associate in the same institution. Collection of the samples was partly funded by grants to K.O. Reinhart from Highlands Biological Station and from the A.W. Mellon Foundation helping form a partnership between the National Park Service, the Ecological Society of America and the National Park Foundation. We are grateful for the kind collaboration of many foresters and scientists who helped collecting leaves of P. serotina and P. virginiana: P.J. Alexander and D. Bailey (New Mexico State University), M. Campbell (Pennsylvania State University), F. Caronni and L. Hildebrand (Parco Lombardo della Valle del Ticino), G. Decocq (University of Picardie), R.B. Kaul and D.M. Sutherland (University of Nebraska), R.A. Klips (Ohio State University), P. Jenkins and J. Tedford (University of Arizona), D.A. Lewis (Iowa State University), N.P. Revsbech (University of Aarhus). We also thank D. Potter and an anonymous reviewer for their constructive comments.

References

  1. Bandelt HJ, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:37–48PubMedGoogle Scholar
  2. Basic N, Besnard G (2006) Gene polymorphisms for elucidating the genetic structure of the heavy-metal hyperaccumulating trait in Thlaspi caerulescens and their cross-genera amplification in Brassicaceae. J Plant Res 119:479–487. doi: 10.1007/s10265-006-0011-x PubMedCrossRefGoogle Scholar
  3. Besnard G (2008) Chloroplast DNA variations in Mediterranean olive. J Hortic Sci Biotechnol 83:51–54Google Scholar
  4. Brettin TS, Karle R, Crowe EL, Iezonni AF (2000) Chloroplast inheritance and DNA variation in sweet, sour and ground cherry. J Hered 91:75–79. doi: 10.1093/jhered/91.1.75 PubMedCrossRefGoogle Scholar
  5. Broennimann O, Treier UA, Müller-Schärer H, Thuiller W, Peterson AT, Guisan A (2007) Evidence of climatic niche shift during biological invasion. Ecol Lett 10:701–709. doi: 10.1111/j.1461-0248.2007.01060.x PubMedCrossRefGoogle Scholar
  6. Demesure B, Sodzi N, Petit RJ (1995) A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Mol Ecol 4:129–131. doi: 10.1111/j.1365-294X.1995.tb00201.x PubMedCrossRefGoogle Scholar
  7. EPPO (2007) List of invasive alien plants (accessed on 12/17/2007 from http://www.eppo.org/QUARANTINE/ias_plants.htm)
  8. Freeman JS, Marques CMP, Carocha V, Borralho N, Potts BM, Vaillancourt RE (2007) Origins and diversity of the Portuguese Landrace of Eucalyptus globulus. Ann For Sci 64:639–647. doi: 10.1051/forest:2007042 CrossRefGoogle Scholar
  9. Godefroid S, Phartyal SS, Weyembergh G, Koedam N (2005) Ecological factors controlling the abundance of non-native invasive black cherry (Prunus serotina) in deciduous forest understory in Belgium. For Ecol Manag 210:91–105. doi: 10.1016/j.foreco.2005.02.024 CrossRefGoogle Scholar
  10. Goudet J (2005) Fstat (version 2.9.4): a program to estimate and test population genetics parameters. http://www2.unil.ch/popgen/softwares/fstat.htm
  11. Harbourne ME, Douglas GC, Waldren S, Hodkinson TR (2005) Characterization and primer development for amplification of chloroplast microsatellite regions of Fraxinus excelsior. J Plant Res 118:339–345. doi: 10.1007/s10265-005-0223-5 PubMedCrossRefGoogle Scholar
  12. Hough AF (1965) Black Cherry (Prunus serotina Hehrh.). Silvics of forest trees of the United States. In: Fowells HA (ed) Agricultures handbook 271. US Department of Agriculture, Washington, DC, pp 539–545Google Scholar
  13. Little EL Jr (1971) Atlas of United States trees, vol 1, conifers and important hardwoods. US Department of Agriculture Miscellaneous Publication No. 1146, WashingtonGoogle Scholar
  14. Magri D, Fineschi S, Bellarosa R, Buonamici A, Sebastiani F, Schirone B, Simeone MC, Vendramin GG (2007) The distribution of Quercus suber chloroplast haplotypes matches the palaeogeographical history of the western Mediterranean. Mol Ecol 16:5259–5266PubMedCrossRefGoogle Scholar
  15. Marquis DA (1975) The Allegheny hardwood forests of Pennsylvania. USDA Forest Service, general technical report NE-15. Northeastern Forest Experiment Station, Upper Darby, 32pGoogle Scholar
  16. Mau-Crimmins TM, Schussman HR, Geiger EL (2006) Can the invaded range of a species be predicted sufficiently using only native-range data? Lehmann lovegrass (Eragrostis lehmanniana) in the southwestern United States. Ecol Model 93:736–746. doi: 10.1016/j.ecolmodel.2005.09.002 CrossRefGoogle Scholar
  17. McVaugh R (1951) A revision of the north American black cherries (Prunus serotina Ehrh., and relatives). Brittonia 7:279–315. doi: 10.2307/2804698 CrossRefGoogle Scholar
  18. Mohanty A, Martín JP, González LM, Aguinagalde I (2003) Association between chloroplast DNA and mitochondrial DNA haplotypes in Prunus spinosa L. (Rosaceae) populations across Europe. Ann Bot (Lond) 92:749–755. doi: 10.1093/aob/mcg198 Google Scholar
  19. Pairon M, Chabrerie O, Mainer Casado C, Jacquemart AL (2006) Sexual regeneration traits linked to black cherry (Prunus serotina Ehrh.) invasiveness. Acta Oecol 30:238–247. doi: 10.1016/j.actao.2006.05.002 CrossRefGoogle Scholar
  20. Pairon M, Jacquemart AL, Potter D (2008) Detection and characterization of genome-specific microsatellite markers in the allotetraploid Prunus serotina. J Am Soc Hortic Sci 133:390–395Google Scholar
  21. Petit RJ, Duminil J, Fineschi S, Hampe A, Salivini D, Vendramin GG (2005) Comparative organization of chloroplast, mitochondrial and nuclear diversity in plant populations. Mol Ecol 14:689–701. doi: 10.1111/j.1365-294X.2004.02410.x PubMedCrossRefGoogle Scholar
  22. Shaw J, Small RL (2004) Addressing the ‘hardest puzzle in American pomology:’ phylogeny of Prunus sect. Prunocerasus (Rosaceae) based on seven noncoding chloroplast DNA regions. Am J Bot 91:985–996. doi: 10.3732/ajb.91.6.985 CrossRefGoogle Scholar
  23. Starfinger U (2006) NOBANIS—invasive alien species fact sheet—Prunus serotina. Online database of the North European and Baltic network on invasive alien species (accessed on 12/17/2007 from www.nobanis.org)
  24. Starfinger U, Kowarik I (2003) Prunus serotina Ehrh. (Rosaceae), Späte Traubenkirsch. Neoflora fact sheet (accessed on 01/22/2008 from http://www.floraweb.de/neoflora/handbuch/prunusserotina.pdf)
  25. Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109. doi: 10.1007/BF00037152 PubMedCrossRefGoogle Scholar
  26. Vendramin GG, Fady B, González-Martínez SC, Sheng Hu F, Scotti I, Sebastiani F, Soto A, Petit RJ (2008) Genetically depauperate but widespread: the case of an emblematic Mediterranean pine. Evolution Int J Org Evol 62:680–688. doi: 10.1111/j.1558-5646.2007.00294.x Google Scholar
  27. Weising K, Gardner RC (1999) A set of conserved PCR primers for the analysis of simple sequence repeat polymorphisms in chloroplast genomes of dicotyledonous angiosperms. Genome 42:9–19. doi: 10.1139/gen-42-1-9 PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • B. Petitpierre
    • 1
  • M. Pairon
    • 2
  • O. Broennimann
    • 1
  • A. L. Jacquemart
    • 2
  • A. Guisan
    • 1
  • Guillaume Besnard
    • 1
    • 3
  1. 1.Department of Ecology and Evolution, BiophoreUniversity of LausanneLausanneSwitzerland
  2. 2.Research Team Genetics, Reproduction, Populations, Biodiversity Research CenterUniversité catholique de LouvainLouvain-la-NeuveBelgium
  3. 3.Imperial College LondonAscotUnited Kingdom

Personalised recommendations