Effects of Rootstock and Interstock on Pollen Traits and Flowering Synchronization in Pistachio (Pistacia vera L.)

  • Mohammad Javad Mahmoudi MeimandEmail author
  • Mohammad Hosein Shamshiri
Original Article


An experiment was carried out in Khatam region, Yazd province, Iran from 2008–2016 where two popular pistachio rootstocks and one interstock were evaluated with one male scion to synchronize flowering period of the late flowering ‘Akbari’ as female and medium flowering ‘M1’ as male. In this experiment, ‘Badami-e-Zarand’ (B) and ‘Fandoghi’ (F) were used as rootstocks and ‘Akbari’ (A) as interstock and three grafting combinations were examined including B/M1, F/M1 and B/A/M1. In terms of flowering time, our results showed that the B/A/M1 combination could delay the flowering in male genotype and make an overlap of 6 days between flowering period of ‘Akbari’, as female and M1 as male pistachio (24–29 March). Rootstocks had no significant effect on flowering time of M1. Pollen tube length and growth rate was influenced by different grafting combinations while pollen germination percentage was similar between treatments.


Pistachio ‘Akbari’ cvs Interstock Male pistachio Pollen 

Einflüsse von Unterlage und Zwischenveredlung auf Polleneigenschaften und Synchronisierung der Blütezeit bei Pistazie (Pistacia vera L.)


Pistaziensorte ‘Akbari’ Zwischenveredlung Männlicher Pistazienbaum Pollen 



We thank Vali-e-Asr University of Rafsanjan for providing support for this research.

Conflict of interest

M.J. Mahmoudi Meimand and M.H. Shamshiri declare that they have no competing interests.


  1. Aloni B, Cohen R, Karni L, Aktas H, Edelstein M (2010) Hormonal signaling in rootstock–scion interactions. Sci Hortic 127:119–126CrossRefGoogle Scholar
  2. Bagheri V, Shamshiri MH, Shirani H, Roosta H (2012) Nutrient uptake and distribution in mycorrhizal pistachio seedlings under drought stress. J Agric Sci Tech 14:1591–1604Google Scholar
  3. Bai S, Kasai A, Yamada K, Li T, Harada T (2011) A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner. J Exp Bot 62(13):4561–4570. CrossRefGoogle Scholar
  4. Bica D, Gay G, Morando A, Soave E (2000) Effects of rootstock and Vitis vinifera genotype on photosynthetic parameters. Acta Hortic 526:373–380. CrossRefGoogle Scholar
  5. Corbesier L, Vincent C, Jang S, Fornara F, Fan Q, Searle I, Giakountis A, Farrona S, Gissot L, Turnbull C (2007) FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis. Science 316:1030–1033CrossRefGoogle Scholar
  6. El-Sharkawy I, El Kayal W, Prasath D, Fernandez H, Bouzayen M, Svircev AM, Jayasankar S (2012) Identification and genetic characterization of a gibberellin 2‑oxidase gene that controls tree stature and reproductive growth in plum. J Exp Bot 63:1225–1239CrossRefGoogle Scholar
  7. Fallahi E, Colt WM, Fallahi B, Chun IJ (2002) The importance of apple rootstocks on tree growth, yield, fruit quality, leaf nutrition, and photosynthesis with an emphasis on ’Fuji’. Hort Technol 12:38–44Google Scholar
  8. Foster TM, Watson AE, Van Hooijdonk BM, Schaffer RJ (2014) Key flowering genes including FT-like genes are upregulated in the vasculature of apple dwarfing rootstocks. Tree Genet Genom 10:189–202CrossRefGoogle Scholar
  9. Gainza F, Opazo I, Guajardo V, Meza P, Ortiz M, Pinochet J, Muñoz C (2015) Rootstock breeding in Prunus species: Ongoing efforts and new challenges. Chil J Agric Res 75:6–16CrossRefGoogle Scholar
  10. Gregory PJ, Atkinson CJ, Bengough AG, Else MA, Fernández-Fernández F, Harrison RJ, Schmidt S (2013) Contributions of roots and rootstocks to sustainable, intensified crop production. J Exp Bot 64:1209–1222CrossRefGoogle Scholar
  11. Ham BK, Brandom JL, Xoconostle-Cázares B, Ringgold V, Lough TJ, Lucas WJ (2009) A polypyrimidine tract binding protein, pumpkin RBP50, forms the basis of a phloem-mobile ribonucleoprotein complex. Plant Cell 21:197–215CrossRefGoogle Scholar
  12. Harada T (2010) Grafting and RNA transport via phloem tissue in horticultural plants. Sci Hortic 125:545–550CrossRefGoogle Scholar
  13. Hedhly A, Hormaza J, Herrero M (2005) Influence of genotype-temperature interaction on pollen performance. J Evol Biol 18:1494–1502CrossRefGoogle Scholar
  14. Holdaway-Clarke TL, Hepler PK (2003) Control of pollen tube growth: Role of ion gradients and fluxes. New Phytol 159:539–563CrossRefGoogle Scholar
  15. Jackson JE (2003) The biology of apples and pears. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  16. Jiménez S, Pinochet J, Gogorcena Y, Betrán J, Moreno M (2007) Influence of different vigour cherry rootstocks on leaves and shoots mineral composition. Sci Hortic 112:73–79CrossRefGoogle Scholar
  17. Karimi HR, Zeraatkar H (2016) Effects of artificial pollination using pollen suspension spray on nut and kernel quality of pistachio cultivar Owhadi. Int J Fruit Sci 16:171–181CrossRefGoogle Scholar
  18. Kubota C, McClure MA, Kokalis-Burelle N, Bausher MG, Rosskopf EN (2008) Vegetable grafting: History, use, and current technology status in North America. HortScience 43:1664–1669CrossRefGoogle Scholar
  19. Nagy Tóth E, Orosz-Kovács ZS (2004) Effect of rootstocks on floral and pollination biological types of apple cultivars. Acta Hortic 636:387–394. CrossRefGoogle Scholar
  20. Ozeker E, Isfendiyaroglu M, Misirli A (2006) Comparison of different Pistacia spp. in terms of pollination biology in the Yunt mountains of Manisa province in Turkey. Pak J Biol Sci 9:371–376CrossRefGoogle Scholar
  21. Ozturk A, Ozturk B (2014) The rootstock influences growth and development of ‘Deveci’ pear. Türk Tarım Ve Doğa Bilimleri 6:1049–1053Google Scholar
  22. Pontikis C (1989) Effects of hydrogen cyanamide on bloom advancement in female pistachio (Pistacia vera L.). Fruit Var J 43:125–128Google Scholar
  23. Porlingis I, Voyiatzis D (1986) Flower synchronization of staminate and pistillate pistachio trees (Pistacia vera L.) with paclobutrazol. Acta Hortic 179:521–528. CrossRefGoogle Scholar
  24. Procopiou J (1973) The induction of earlier blooming in female pistachio trees by mineral oil-DNOC winter sprays. J Hortic Sci 48(4):393–395. CrossRefGoogle Scholar
  25. Radice S, Ontivero M, Andorno A, Dessy S (2004) Floral morphology and pollen viability of the ’Forastero’ cultivar [Prunus persica L. Batsch], as modified by the rootstock. Acta Hortic 658:81–85. CrossRefGoogle Scholar
  26. Turker S, Ak B (2010) Effects of different rootstocks on physical traits of Siirt and Ohadi pistachio cultivars. In: Zakynthinos G (ed) XIV GREMPA Meeting on Pistachios and Almonds Zaragoza. Options Méditerranéennes : Série A. Séminaires Méditerranéens, vol 94, pp 245–250Google Scholar
  27. Zeraatkar H, Karimi HR, Shamshiri MH, Tajabadipur A (2013) Preliminary evaluation of artificial pollination in pistachio using pollen suspension spray. Plant Knowl J 2(3):94–98Google Scholar

Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2019

Authors and Affiliations

  • Mohammad Javad Mahmoudi Meimand
    • 1
    Email author
  • Mohammad Hosein Shamshiri
    • 1
  1. 1.Department of Horticulture, College of AgricultureValiasr University of RafsanjanRafsanjanIran

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