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Erwerbs-Obstbau

, Volume 60, Supplement 1, pp 55–59 | Cite as

Influence of BAP Concentrations and Nutrient Medium Composition on In Vitro Regeneration of ‘Öküzgözü’ and ‘Boğazkere’ (Vitis vinifera L.) Cultivars

  • Hakan Yildirim
  • Gultekin OzdemirEmail author
Original Article
  • 60 Downloads

Abstract

This study has been conducted with the aim to determine the type of nutrient medium that can be used in micropropagation studies for ‘Öküzgözü’ and ‘Boğazkere’ and to specify BAP concentrations. In the study where ejectors with a length of 0.7–0.8 cm that are obtained with single-node culture are used, it was focused on four different nutrient media such as MS, DKW, QL and WPM and on six different concentrations such as 0.2–0.4–0.6–0.8–1.0–1.5 mg l−1 BAP. Single-node suspension explants which will be used in initiating the culture, are taken into culture in MS nutrient medium and the nutrient medium is supported with 30 g l−1 sucrose, 6 g l−1 agar and 1 mg l−1 BAP. In the trial environment, parameters such as number of shoots, shoot length (cm), number of nodes and callus ratio have been investigated. For both grape varieties, the best outcome was obtained with MS nutrient medium with respect to number of shoots, shoot length, and number of nodes. These values were found as 4.66, 1.24 and 6.39 for ‘Öküzgözü’ variety respectively, whereas they are determined as 6.28, 1.15 and 6.81 for ‘Boğazkere’ variety respectively. In both grape varieties in DKW nutrient medium, starting from the 2nd week of culture, obscuration began to appear on the shoots and after this stage no other development has taken place.

Keywords

‘Öküzgözü’ ‘Boğazkere’ In vitro BAP Nutrient medium 

Einfluss von BAP-Konzentrationen und Nährmedienzusammensetzung auf die in vitro Revitalisierung bei den Weinreben-Sorten Öküzgözü’ und ‘Boğazkere’ (Vitis vinifera L.)

Schlüsselwörter:

‘Öküzgözü’ ‘Boğazkere’ In vitro BAP Nährmedium 

Notes

Conflict of interest

H. Yildirim and G. Ozdemir declare that they have no competing interests.

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Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2018

Authors and Affiliations

  1. 1.Agriculture Faculty Horticulture DepartmentInonu UniversityMalatyaTurkey
  2. 2.Agriculture Faculty Horticulture DepartmentDicle UniversityDiyarbakirTurkey

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