Journal of Pest Science

, Volume 93, Issue 1, pp 427–437 | Cite as

Associational resistance to a pest insect fades with time

  • Bastien CastagneyrolEmail author
  • Mikhail V. Kozlov
  • Charlotte Poeydebat
  • Maude Toïgo
  • Hervé Jactel
Original Paper


Tree diversity is one of the drivers of forest resistance to herbivores. Most of the current understanding of the diversity resistance relationship comes primarily from short-term studies. Knowing whether tree diversity effects on herbivores are maintained over time is important for perennial ecosystems like forests. We addressed the temporal dynamics of the diversity resistance relationship by conducting a 6-year survey of pine attacks by the pine processionary moth Thaumetopoea pityocampa (PPM) in a tree diversity experiment where we could disentangle tree composition from host density effects. During the first years after planting the trees, PPM attacks on maritime pine Pinus pinaster were reduced in the presence of birch Betula pendula, a fast-growing non-host tree (i.e. associational resistance). This effect was maintained but faded with time as the pines eventually grew taller than neighbouring birches. The number of repeated attacks on individual pine trees also decreased in mixed pine–birch stands. Pine density had a positive effect on stand colonisation by PPM and a negative effect on the proportion of trees that were attacked. Pines were less likely to be repeatedly attacked as pine density increased, with attacks being spread over a larger number of host trees. Collectively, these results unravel the independent contribution of tree species composition and host density to tree resistance to herbivores. Both processes had directional changes over time. These results indicate that the resistance of planted forests to herbivores can be improved by carefully choosing the composition of mixed forests and the timing of species planting.


Associational effects Forest ORPHEE experiment Plant–insect interactions Thaumetopoea pityocampa 



BC was supported by the GIP-ECOFOR programme from the French ministry of agriculture (Project BIOPICC ECOFOR-2014-15). MT, CP and HJ received support from the French National Research agency (Project DiPTiCC, ANR-16-CE32-0003-01). MVK was supported by the Academy of Finland (Projects 311929 and 316182). We thank people who contributed to count PPM nests since 2013 (with no particular order): Maxime Damien, Inge van Halder, Margot Régoloni, Céline Meredieu, Fabrice Vétillard, Lucile Perrot, Angelina Ceballos-Escalera, Yasmine Kadiri, Christophe Poilleux and Victor Rebillard. The authors acknowledge UEFP 0570, INRA, 69 route d’Arcachon, 33612, CESTAS, for the management of the ORPHEE experiment.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.

Supplementary material (6.3 mb)
Supplementary material 1 (ZIP 6418 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.BIOGECO, INRAUniv. BordeauxCestasFrance
  2. 2.Section of Ecology, Department of BiologyUniversity of TurkuTurkuFinland
  3. 3.CEFE, CNRS, EPHE, IRDUniv Montpellier, Univ Paul Valéry Montpellier 3MontpellierFrance

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