Screening the variability in oilseed rape resistance to pollen beetle attacks in the field and assessment of biochemical biomarkers
The pollen beetle (Brassicogethes aeneus) is one of the main insect pests affecting oilseed rape crops. Efficiency of insecticides used to control this pest is decreasing due to the development of resistance to compounds such as pyrethroids in many populations. Breeding oilseed rape for resistance to pollen beetle attacks could be an interesting strategy to find alternative control methods but has not been really developed in this crop yet. However, screening plants for insect resistance remains complicated as it often involves field tests on large genotype collections which are complicated to carry out without biases. Current knowledge on the chemical ecology of interactions between oilseed rape and pollen beetles could help finding biochemical markers of this resistance and bypass this problematic field screening phase, thus allowing an indirect breeding approach. Previous laboratory tests have shown that variations in attack levels among a small set of oilseed genotypes could be explained by the biochemistry of bud tissues. The present study aimed at validating this link under field conditions. For that purpose, we conducted a multi-site experiment in France with 19 genotypes exposed to pollen beetle attacks. We phenotyped pollen beetle damage and sampled buds in the field to assess their chemical composition. Large variability in pollen beetle attacks was observed over the genotypes. These attack levels were consistent between locations. Bud chemistry was highly variable, but most compounds were well correlated between locations. Potential biomarkers previously identified in laboratory experiments were not confirmed to be correlated with resistance to pollen beetles in the field, but new compounds which may be considered interesting markers for resistance screening against the pollen beetle emerged.
KeywordsPhenotyping Metabolite Plant resistance Brassicogethes aeneus Brassica napus
We are very grateful to Nathalie Marnet, Catherine Jonard and Solene Berardocco for preparation and management of chemical analyses. All chemical analyses were performed on the P2M2 platform (Le Rheu, France). We would like to thank people from Biogemma: Pierre George and Xavier Heudelot who managed field trials, Isabelle André who participated in sample preparation and Michèle Barthes and Fabienne Mezzasalma for sowing preparation. We also thank Marie Coque and Guillaume Hostyn, who participated in the development of this project. We are grateful to Günter Leis and Thibaut Cordette from Limagrain who maintained field trials and participated in damage estimation. We are thankful to Loïc Daniel and Thomas Hecky and Kathleen Menacer who participated in sample collection and preparation, Eloïse Couthouis and Guillaume Audo who contributed to sample preparation. Gaëtan Seimandi Corda was supported by Biogemma and the French National Association Research and Technology (CIFRE N° 2014/1354).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants. All applicable international, national and/or institutional guidelines for the care and use of animals were followed.
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