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Journal of Pest Science

, Volume 90, Issue 1, pp 369–378 | Cite as

The essential oil from Zanthoxylum monophyllum a potential mosquito larvicide with low toxicity to the non-target fish Gambusia affinis

  • Roman Pavela
  • Marimuthu Govindarajan
Original Paper

Abstract

The mosquito larvicidal activity of Zanthoxylum monophyllum leaf essential oil (EO) and its major chemical constituents was tested against the three mosquito vectors Anopheles subpictus, Aedes albopictus and Culex tritaeniorhynchus. In the EO of Z. monophyllum, it contains 36 compounds with the two major compounds being Germacrene D-4-ol (19.40 %) and α-Cadinol (12.30). The larvicidal activity of the essential oil against An. subpictus, Ae. albopictus and Cx. tritaeniorhynchus was determined and LC50 values were estimated at 41.50, 45.35 and 49.01 µg/mL, respectively. The two major compounds Germacrene D-4-ol and α-Cadinol were tested for acute toxicity against larvae of the three mosquito vectors. Germacrene D-4-ol showed a significantly higher efficacy compared to α-Cadinol. While LC50 for Germacrene D-4-ol ranged from 6.12 to 7.26 µg/mL, LC50 values for α-Cadinol were estimated in the range from 10.27 to 12.28 µg/mL. The EO, Germacrene D-4-ol and α-Cadinol were found safer to the non-target organism Gambusia affinis (LC50 = 4234.07, 414.05 and 635.12 µg/mL, respectively), which was manifested in the high suitability of the index/predator safety factor value, ranging from 86.36 for the least sensitive larvae of Cx. tritaeniorhynchus to 102.02 for the most sensitive larvae of An. subpictus.

Keywords

Eco-friendly larvicides Mosquito vectors Plant-borne mosquitocides Botanical insecticides 

Notes

Acknowledgments

The authors would like to thank Dr. R. Karuppasamy, Professor and Head of the Department of Zoology at Annamalai University for the laboratory facilities provided. We also acknowledge the cooperation of the staff members from the VCRC (ICMR), Pondicherry. Dr. Pavela, as co-author, would like to thank the Ministry of Agriculture of the Czech Republic for its financial support concerning botanical pesticide research (Project No. MZE RO0415).

Compliance with ethical standards

Conflicts of interest

The authors declare no conflicts of interest.

Ethical approval

All applicable international and national guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards and practices of the institution at which the studies were conducted.

References

  1. Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267CrossRefGoogle Scholar
  2. Adesina SK (2005) The Nigerian Zanthoxylum; chemical and biological values. Afr J Tradit Complement Altern Med 2:282–301Google Scholar
  3. Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils—a review. Food Chem Toxicol 46:446–475CrossRefPubMedGoogle Scholar
  4. Barkatullah Ibrar M, Muhammad N, Ur-Rehman I, Rehman MU, Khan A (2013) Chemical composition and biological screening of essential oils of Zanthoxylum armatum DC leaves. J Clin Toxicol 3:172. doi: 10.4172/2161-0495.1000172 Google Scholar
  5. Benelli G (2015a) Research in mosquito control: current challenges for a brighter future. Parasitol Res 114:2801–2805CrossRefPubMedGoogle Scholar
  6. Benelli G (2015b) Plant-borne ovicides in the fight against mosquito vectors of medical and veterinary importance: a systematic review. Parasitol Res 114:3201–3212CrossRefPubMedGoogle Scholar
  7. Benelli G, Murugan K, Panneerselvam C, Madhiyazhagan P, Conti B, Nicoletti M (2015) Old ingredients for a new recipe? Neem cake, a low-cost botanical by-product in the fight against mosquito-borne diseases. Parasitol Res 114:391–397CrossRefPubMedGoogle Scholar
  8. Borah R, Saikia K, Talukdar AK, Kalita MC (2012) Chemical composition and biological activity of the leaf essential oil of Zanthoxylum oxyphyllum. Planta Med 78:P_100CrossRefGoogle Scholar
  9. Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 94:223–253CrossRefPubMedGoogle Scholar
  10. Conti B, Flamini G, Cioni PL, Ceccarini L, Leonardi MM, Benelli G (2014) Mosquitocidal essential oils: are they safe against non-target aquatic organisms? Parasitol Res 113:251–259CrossRefPubMedGoogle Scholar
  11. Cuca LE, Martinez JC, Monache FD (1998) Constituyentes químicos de Zanthoxylum monophyllum. Rev Colomb Quim 27:17–27Google Scholar
  12. De García LA, Calle J, Reguero MT, Nathan PJ (1989) Phytochemical study of Zanthoxylum monophyllum. Fitoterapia 60:447–448Google Scholar
  13. Deo PG, Hasan SB, Majumdar SK (1988) Toxicity and suitability of some insecticides for household use. Int Pest Control 30:118–129Google Scholar
  14. Dias N, Moraes DFC (2014) Essential oils and their compounds as Aedes aegypti L. (Diptera: Culicidae) larvicides: review. Parasitol Res 113:565–592CrossRefPubMedGoogle Scholar
  15. Finney DJ (1971) Probit analysis. Cambridge University Press, LondonGoogle Scholar
  16. Gilani SN, Khan AU, Gilani AH (2010) Pharmacological basis for the medicinal use of Zanthoxylum armatum in gut, airways and cardiovascular disorders. Phytother Res 24:553–558PubMedGoogle Scholar
  17. Gómez Y, Gil K, González E, Farías LM (2007) Actividad antifúngica de extractos orgánicos del árbol Fagara monophylla (Rutaceae) en Venezuela. Int J Trop Biol 55:767–775Google Scholar
  18. Govindarajan M (2010) Chemical composition and larvicidal activity of leaf essential oil from Clausena anisata (willd.) Hook. F. Benth (Rutaceae) against three mosquito species. Asian Pac J Trop Med 3:874–877CrossRefGoogle Scholar
  19. Govindarajan M (2011) Larvicidal and repellent properties of some essential oils against Culex tritaeniorhynchus Giles and Anopheles subpictus Grassi (Diptera: Culicidae). Asian Pac J Trop Med 4:106–111CrossRefPubMedGoogle Scholar
  20. Govindarajan M, Sivakumar R, Rajeswari M, Yogalakshmi K (2012) Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species. Parasitol Res 110:2023–2032CrossRefPubMedGoogle Scholar
  21. Govindarajan M, Sivakumar R, Rajeswary M, Yogalakshmi K (2013) Chemical composition and larvicidal activity of essential oil from Ocimum basilicum (L.) against Culex tritaeniorhynchus, Aedes albopictus and Anopheles subpictus (Diptera: Culicidae). Exp Parasitol 134:7–11CrossRefPubMedGoogle Scholar
  22. Govindarajan M, Rajeswary M, Hoti SL, Benelli G (2016) Larvicidal potential of carvacrol and terpinen-4-ol from the essential oil of origanum vulgare (lamiaceae) against Anopheles stephensi, Anopheles subpictus, Culex quinquefasciatus and culex tritaeniorhynchus (Diptera: Culicidae). Res Vet Sci. doi: 10.1016/j.rvsc.2015.11.011 Google Scholar
  23. Hemingway J, Ranson H (2000) Insecticide resistance in insect vectors of human disease. Ann Rev Entomol 45:371–391CrossRefGoogle Scholar
  24. Isman MB (2006) Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annu Rev Entomol 51:46–66CrossRefGoogle Scholar
  25. Isman MB, Grieneisen ML (2014) Botanical insecticide research: many publications, limited useful data. Trends Plant Sci 19:140–145CrossRefPubMedGoogle Scholar
  26. Lawrence C, Rutherford N, Hamilton R, Meredith D (2016) Experimental evidence indicates that native freshwater fish outperform introduced Gambusia in mosquito suppression when water temperature is below 25°C. Hydrobiologia 766:357–364CrossRefGoogle Scholar
  27. Liu XC, Liu QY, Zhou L, Liu QR, Liu ZL (2014) Chemical composition of Zanthoxylum avicennae essential oil and its larvicidal activity on Aedes albopictus Skuse. Trop J Pharm Res 13:399–404CrossRefGoogle Scholar
  28. McCaffery A, Nauen R (2006) The Insecticide Resistance Action Committee (IRAC): public responsibility and enlightened industrial self interest. Outlook Pest Manag 17:11–14Google Scholar
  29. Nerio LS, Olivero-Verbela J, Stashenko E (2010) Repellent activity of essential oils: a review. Bioresour Technol 101:372–378CrossRefPubMedGoogle Scholar
  30. Pavela R (2007) Lethal and sublethal effects of thyme oil (Thymus vulgaris L.) on the house fly (Musca domestica Lin.). J Essent Oil Bear Pl 10:346–356CrossRefGoogle Scholar
  31. Pavela R (2011) Insecticidal properties of phenols on Culex quinquefasciatus Say and Musca domestica L. Parasitol Res 190:1547–1553CrossRefGoogle Scholar
  32. Pavela R (2014a) Insecticidal properties of Pimpinella anisum essential oils against the Culex quinquefasciatus and the non-target organism Daphnia magna. J Asia Pac Entomol 17:287–293CrossRefGoogle Scholar
  33. Pavela R (2014b) Acute, synergistic and antagonistic effects of some aromatic compounds on the Spodoptera littoralis Boisd. (Lep., Noctuidae) larvae. Ind Crop Prod 60:247–258CrossRefGoogle Scholar
  34. Pavela R (2015a) Essential oils for the development of eco-friendly mosquito larvicides: A review. Ind Crops Prod 76:174–187CrossRefGoogle Scholar
  35. Pavela R (2015b) Acute toxicity and synergistic and antagonistic effects of the aromatic compounds of some essential oils against Culex quinquefasciatus Say larvae. Parasitol Res 114:3835–3853CrossRefPubMedGoogle Scholar
  36. Pavela R, Vrchotova N, Triska J (2009) Mosquitocidal activities of thyme oils (Thymus vulgaris L.) against Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 105:1365–1370CrossRefPubMedGoogle Scholar
  37. Pirani JRA (1993) New species and new combination in Zanthoxylum (Rutaceae) from Brazil. Brittonia 45:154–158CrossRefGoogle Scholar
  38. Pitasawat B, Champakaew D, Choochote W (2007) Aromatic plant-derived essential oil: an alternative larvicide for mosquito control. Fitoterapia 78:205–210CrossRefPubMedGoogle Scholar
  39. Prakash B, Singh P, Mishra PK, Dubey NK (2012) Safety assessment of Zanthoxylum alatum Roxb. essential oil, its antifungal, antiaflatoxin, antioxidant activity and efficacy as antimicrobial in preservation of Piper nigrum L. fruits. Int J Food Microbiol 153:183–191CrossRefPubMedGoogle Scholar
  40. Prieto JA, Patiño OJ, Delgado WA, Moreno JP, Cuca LE (2011) Chemical composition, insecticidal, and antifungal activities of fruit essentials oils of free Colombian Zanthoxylum species. Chil J Agric Res 71:73–82CrossRefGoogle Scholar
  41. Quesada RM, Fernández JV (2005) Actualización de listado de especies arbóreas de uso forestal y otros usos en Costa Rica. Kurú: Revista Forestal (Costa Rica) 2:1–45Google Scholar
  42. Regnault-Roger C, Vincent C, Arnason JT (2012) Essential oils in insect control: low-risk products in a high-stakes world. Ann Rev Entomol 57:405–424CrossRefGoogle Scholar
  43. Rodríguez-Guzmán R, Johansmann Fulks LC, Radwan MM, Burandt CL, Ross SA (2011) Chemical constituents, antimicrobial and antimalarial activities of Zanthoxylum monophyllum. Plant Med 77:1542–1544CrossRefGoogle Scholar
  44. Setzer WN, Noletto JA, Lawton RO, Haber WA (2005) Leaf essential oil composition of five Zanthoxylum species from Monteverde, Costa Rica. Mol Divers 9:3–13CrossRefPubMedGoogle Scholar
  45. Seyoum A, Killeen GF, Kabiru EW, Knols BGJ, Hassanali A (2003) Field efficacy of thermally expelled or live potted repellent plants against African malaria vectors in western Kenya. Tropic Med Int Health 8:1005–1011CrossRefGoogle Scholar
  46. Shahi M, Kamrani E, Salehi M, Habibi R, Hanafi-Bojd AA (2015) Native Larvivorous Fish in an endemic malarious area of Southern Iran, a biological alternative factor for chemical larvicides in malaria control program. Iran J Publ Health 44:1544–1549Google Scholar
  47. Sivagnaname N, Kalyanasundaram M (2004) Laboratory evaluation of methanolic extract of Atlantia monophylla (Family: Rutaceae) against immature stages of mosquitoes and non-target organisms. Mem Inst Oswaldo Cruz 99:115–118CrossRefPubMedGoogle Scholar
  48. Soderlund DM, Clark JM, Sheets LP, Mullin LS, Piccirillo VJ, Sargent D, Stevens JT, Weiner ML (2002) Mechanisms of pyrethroid neurotoxicity: implications for cumulative risk assessment. Toxicol 171:3–59CrossRefGoogle Scholar
  49. Stroh J, Wan MT, Isman MB, Moul DL (1998) Evaluation of the acute toxicity to juvenile pacific coho salmon and rainbow trout of some plant essential oils, a formulated product, and the carrier. Bull Environ Contam Toxicol 60:923–930CrossRefPubMedGoogle Scholar
  50. Tiwary M, Naik SN, Tewary DK, Mittal PK, Yadav S (2007) Chemical composition and larvicidal activities of the essential oil of Zanthoxylum armatum DC (Rutaceae) against three mosquito vectors. J Vector Borne Dis 44:198–204PubMedGoogle Scholar
  51. Walker K, Lynch M (2007) Contributions of Anopheles larval control to malaria suppression in tropical Africa: review of achievements and potential. Med Vet Entomol 21:2–21CrossRefPubMedGoogle Scholar
  52. WHO (World Health Organization) (2005) Guidelines for laboratory and field testing of mosquito larvicides. WHO/CDS/WHOPES/GCDPP/2005.13Google Scholar
  53. WHO (World Health Organization) (2012) Global plan for insecticide resistance management in malaria vectors (GPIRM). http://whqlibdoc.who.int/Publications/2012/9789241564472_eng.pdf. Accessed 15 Apr 2012

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Crop Research InstitutePrague 6Czech Republic
  2. 2.Unit of Vector Control, Phytochemistry and Nanotechnology, Department of ZoologyAnnamalai UniversityChidambaramIndia

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