Journal of Pest Science

, Volume 89, Issue 4, pp 993–1001 | Cite as

Fungal endophytes as promising tools for the management of bean stem maggot Ophiomyia phaseoli on beans Phaseolus vulgaris

  • Beritah Mutune
  • Sunday Ekesi
  • Saliou Niassy
  • Viviene Matiru
  • Christine Bii
  • Nguya K. Maniania
Original Paper


Common bean, Phaseolus vulgaris, is an important food and cash crop in Africa. Its production is seriously affected by the bean stem maggot (BSM), Ophiomyia spp., which attacks seedlings. We evaluated the ability of eleven fungal isolates to colonize bean plants and the effects of inoculation on BSM feeding and oviposition, pupation, and adult emergence. All fungal isolates were able to colonize different bean plant parts (root, stem, and leaves), except isolates of Metarhizium anisopliae and Beauveria bassiana isolate ICIPE 273. Colonization was generally higher on the roots than on the stem and leaves and varied significantly between the fungal isolates. BSM feeding and oviposition were significantly reduced in all the fungus-inoculated bean plants which in turn affected pupation and adult emergence as compared to the control. Metarhizium anisopliae ICIPE 20 outperformed the other isolates in interfering with BSM lifecycle. Although M. anisopliae ICIPE 78 recorded a high number of punctures similar to the control, a significant reduction in the number of pupae and adult emergence was observed, suggesting possible BSM growth inhibition. This study clearly demonstrates that fungal endophytes can be considered as promising tools for the management of BSM in East Africa.


Entomopathogenic fungi Agromyzidae Colonization Feeding Oviposition Adult emergence 



This study was funded icipe’s Innovative Seed Research Grant “Bean Stem Maggot”. The authors are very grateful to Mrs Gloria Muthoni for technical assistance. We acknowledge the statistical analysis assistance from Dr. Daisy Salifu and taxonomical assistance from Dr. Robert Copeland of icipe and Dr. Fathiya Khamis for molecular analysis.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Beritah Mutune
    • 1
    • 2
  • Sunday Ekesi
    • 1
  • Saliou Niassy
    • 1
  • Viviene Matiru
    • 2
  • Christine Bii
    • 2
  • Nguya K. Maniania
    • 1
  1. 1.International Centre of Insect Physiology and Ecology (icipe)NairobiKenya
  2. 2.Jomo Kenyatta University of Agriculture and TechnologyNairobiKenya

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