Journal of Pest Science

, Volume 88, Issue 3, pp 657–663 | Cite as

Cytochrome P450 monooxygenase activity levels in phytoplasma-infected and uninfected Amplicephalus curtulus (Hemiptera: Cicadellidae): possible implications of phytoplasma infections

  • Nolberto L. Arismendi
  • Maritza Reyes
  • Roberto Carrillo
Original Paper

Abstract

The process of pass-through and multiplication of phytoplasma in host-insect tissues could cause some pathogenic effects in insect vectors and may increase the susceptibility to some insecticides. We propose that ‘Candidatus phytoplasma ulmi'-infected Amplicephalus curtulus had reduced cytochrome P450 (P450s) activity compared with uninfected leafhoppers. P450s activity and phytoplasmas were quantified in adult A. curtulus at 25, 35 and 45 days after the access acquisition period (AAP) in the head-thorax and abdomen sections. Real-time PCR analysis showed that 67 and 78 % of insect samples were positive to phytoplasma at 35 and 45 days after the AAP, respectively. None of the samples tested positive to phytoplasma at 25 days after the AAP. P450s activity did not change at 35 days of incubation, but 45 days after the AAP, the enzymatic activity remained 112 % higher in phytoplasma-infected than in noninfected leafhoppers. P450s activity in the abdomen and head-thorax sections in phytoplasma-infected leafhoppers was 28 and 81 % more than in uninfected leafhoppers, respectively. Females had a higher concentration of phytoplasma than males, with 38 % more in the abdomen than in the head-thorax section. These results indicate that infection with ‘Ca. Phytoplasma ulmi' alters A. curtulus P450s activity because of phytoplasma invasion in the host, and it is recognized as probably being an exogenous agent for a specific time period in the life of the insect vector.

Keywords

16SrV-A Cytochrome P450 Phytoplasma-vector relationship Real-time PCR 

References

  1. Arismendi (2014) Transmission of ‘Candidatus Phytoplasma ulmi’ (Elm Yellows, 16SrV-A) by Amplicephalus curtulus (Hemiptera: Cicadellidae) and its effect on the fitness and metabolism of its vector. Doctoral thesis, Universidad Austral de Chile, Chile, p 175Google Scholar
  2. Arismendi N, González F, Zamorano A, Andrade N, Pino AM, Fiore N (2011) Molecular identification of ‘Candidatus Phytoplasma fraxini’ in murta and peony in Chile. Bull Insectol 64:S95–S96Google Scholar
  3. Arismendi N, Riegel R, Carrillo R (2014) In vivo transmission of ‘Candidatus Phytoplasma ulmi’ by Amplicephalus curtulus (Hemiptera: Cicadellidae) and its effect on ryegrass (Lolium multiflorum Cv. Tama). J Econ Entomol 107:83–91CrossRefPubMedGoogle Scholar
  4. Beanland L, Hoy CW, Miller SA, Nault LR (2000) Influence of aster yellows phytoplasma on the fitness of aster leafhopper (Homoptera: Cicadellidae). Ann Entomol Soc Am 93:271–276CrossRefGoogle Scholar
  5. Bosco D, Palermo S, Mason G, Tedeschi R, Marzachi C, Boccardo G (2002) DNA-based methods for the detection and the identification of phytoplasmas in insect vector extracts. Mol Biotechnol 22:9–18CrossRefPubMedGoogle Scholar
  6. Bressan A, Clair D, Sémétey O, Boudon-Padieu E (2005a) Effect of two strains of Flavescence dorée phytoplasma on the survival and fecundity of the experimental leafhopper vector Euscelidius variegatus Kirschbaum. J Invertebr Pathol 89:144–149CrossRefPubMedGoogle Scholar
  7. Bressan A, Girolami V, Boudon-Padieu E (2005b) Reduced fitness of the leafhopper vector Scaphoideus titanus exposed to Flavescence dorée phytoplasma. Entomol Exp Appl 115:283–290CrossRefGoogle Scholar
  8. Buchon N, Broderick NA, Poidevin M, Pradervand S, Lemaitre B (2009) Drosophila intestinal response to bacterial infection: activation of host defense and stem cell proliferation. Cell Host Micr 5:200–211CrossRefGoogle Scholar
  9. Christensen NM, Nicolaisen M, Hansen M, Schulz A (2004) Distribution of phytoplasmas in infected plants as revealed by real-time PCR and bioimaging. Mol Plant Micr Interact 17:1175–1184CrossRefGoogle Scholar
  10. Contaldo N, Bertaccini A, Paltrinieri S, Windsor HM, Windsor GW (2012) Axenic culture of plant pathogenic phytoplasmas. Phytopathol Mediterr 51:607–617Google Scholar
  11. De Sousa GA, Brun A, Amichot M, Rahmani R, Berge J (1995) A microfluorometric method for measuring ethoxycoumarin-O-deethylase activity on individual Drosophila melanogaster abdomens: interest for screening resistance in insect populations. Anal Biochem 229:86–91CrossRefPubMedGoogle Scholar
  12. Després L, David JP, Gallet C (2007) The evolutionary ecology of insect resistance to plant chemicals. Trends Ecol Evol 22:298–307CrossRefPubMedGoogle Scholar
  13. Duron O, Labbe P, Berticat C, Rousset F, Guillot S, Raymond M, Weill M (2006) High Wolbachia density correlates with cost of infection for insecticide resistant Culex pipiens mosquitoes. Evolution 60:303–314CrossRefPubMedGoogle Scholar
  14. Fiore N, Prodan S, Paltrinieri S, Gajardo A, Botti S, Pino AM, Montealegre J, Bertaccini A (2007) Molecular characterization of phytoplasmas in Chilean grapevines. Bull Insectol 60:331–332Google Scholar
  15. Frost KE, Willis DK, Groves RL (2011) Detection and variability of aster yellows phytoplasma titer in tis insect vector, Macrosteles quadrilineatus (Hemiptera: Cicadellidae). J Econ Entomol 104:1800–1815CrossRefPubMedGoogle Scholar
  16. Gonzalez F, Zamorano A, Pino AM, Paltrinieri S, Bertaccini A, Fiore N (2011) Identification of phytoplasma belonging to X-disease group in cherry in Chile. Bull Insectol 64:S235–S236Google Scholar
  17. Gui Z, Hou C, Liu T, Qin G, Li M, Jin B (2009) Effects of insect viruses and pesticides on glutathione s-transferase activity and gene expression in Bombyx mori. J Econ Entomol 102:1591–1598CrossRefPubMedGoogle Scholar
  18. Hepp R, Vargas M (2002) Detección por PCR del agente causal de la marchitez amarilla de la remolacha en cicadélidos (Homóptera: Cicadellidae) asociados al cultivo de la remolacha. Fitopatología 37:67–108Google Scholar
  19. Honĕk A (1993) Intraspecific variation in body size and fecundity in insects: a general relationship. Oikos 66:483–492CrossRefGoogle Scholar
  20. Kontsedalov S, Zchori-Fein E, Chiel E, Gottlieb Y, Inbar M, Ghanim M (2008) The presence of Rickettsia is associated with increased susceptibility of Bemisia tabaci (Homoptera: Aleyrodidae) to insecticides. Pest Manag Sci 64:789–792CrossRefPubMedGoogle Scholar
  21. Li X, Schuler MA, Berenbaum MR (2007) Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics. Annu Rev Entomol 52:231–253CrossRefPubMedGoogle Scholar
  22. Marzachì C, Bosco D (2005) Relative quantification of chrysanthemum yellows (16SrI) phytoplasma in its plant and insect host using real-time polymerase chain reaction. Mol Biotechnol 30:117–127CrossRefPubMedGoogle Scholar
  23. Reyes M, Rocha K, Alarcón L, Siegwart M, Sauphanor B (2012) Metabolic mechanisms involved in the resistance of field populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) to spinosad. Pestic Biochem Physiol 102:45–50CrossRefGoogle Scholar
  24. Schneider B, Seemuller E (1994) Presence of two sets of ribosomal genes in phytopathogenic mollicutes. Appl Environ Microbiol 60:3409–3412PubMedCentralPubMedGoogle Scholar
  25. Tiwari S, Pelz-Stelinski K, Stelinski LL (2010) Effect of ‘Candidatus Liberibacter asiaticus’ infection on susceptibility of Asian citrus psyllid, Diaphorina citri, to selected insecticides. Pest Manag Sci 67:94–99CrossRefPubMedGoogle Scholar
  26. Tiwari S, Pelz-Stelinski K, Mann R, Stelinski L (2011a) Gluthatione transferase and cytochrome P450 (general oxidase) activity levels in ‘Candidatus Liberibacter asiaticus’-infected and uninfected Asian citrus psyllid (Hemiptera: Psyllidae). Ann Entomol Soc Am 104:297–305CrossRefGoogle Scholar
  27. Tiwari S, Gondhalekar AD, Mann RS, Scharf ME, Stelinski LL (2011b) Characterization of five CYP4 genes from Asian citrus psyllid and their expression levels in ‘Candidatus Liberibacter asiaticus’-infected and uninfected psyllids. Insect Mol Biol 20:733–744CrossRefPubMedGoogle Scholar
  28. Weintraub PG, Beanland L (2006) Insect vectors of phytoplasmas. Annu Rev Entomol 51:91–111CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Nolberto L. Arismendi
    • 1
    • 3
  • Maritza Reyes
    • 2
  • Roberto Carrillo
    • 3
  1. 1.Graduate School, Faculty of Agricultural SciencesUniversidad Austral de ChileValdiviaChile
  2. 2.Institut National de la Recherche Agronomique (INRA), UR 406 Abeilles & EnvironnementAvignonFrance
  3. 3.Laboratory of Entomology, Faculty of Agricultural SciencesInstitute of Production and Plant Protection, Universidad Austral de ChileValdiviaChile

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