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Journal of Pest Science

, Volume 88, Issue 1, pp 163–170 | Cite as

Evaluation of entomopathogenic nematodes and their combined application against Curculio elephas and Polyphylla fullo larvae

  • Sevdiye Demir
  • Mehmet Karagoz
  • Selcuk HazirEmail author
  • Harry K. Kaya
Original Paper

Abstract

The larvae of Curculio elephas (Coleoptera: Curculionidae) and Polyphylla fullo (Coleoptera: Scarabaeidae) are major agricultural pests of chestnut and roots of cultivated crops, respectively. Previous research showed that they are relatively resistant to nematode infection. Accordingly, we evaluated the efficacy of Steinernema glaseri, S. weiseri or Heterorhabditis bacteriophora alone or in combination against these two insect pests. A nematode concentration of 50 or 100 infective juveniles (IJs)/larva for C. elephas or 50 or 100 IJs/cm2 for P. fullo at 25 °C was used. The highest (81 %) and the lowest (21 %) larval mortalities for C. elephas were obtained with S. weiseri+H. bacteriophora combined application and S. glaseri alone, respectively. The nature of the interactions (antagonism, additive, or synergy) for the larval mortality was evaluated. For C. elephas, S. weiseri combined with S. glaseri or H. bacteriophora was additive, whereas the combination of S. glaseri+H. bacteriophora was antagonistic. For P. fullo, the efficiency of nematodes used alone or combinations was very low, and there were no significant differences among the treatments at 50 or 100 IJs/cm2. The interaction was additive with the combinations of S. glaseri+H. bacteriophora against P. fullo larvae. No synergistic effect was observed for any combination against C. elephas and P. fullo larvae. Our results show that the EPN species tested, either alone or in combination, are not economically feasible for use against C. elephas or P. fullo, but that further research with other combinations of EPN species or EPNs with other entomopathogens is warranted.

Keywords

Curculio elephas Polyphylla fullo Biological control Entomopathogenic nematodes Steinernema Heterorhabditis 

Notes

Acknowledgments

We thank Dr. Albrecht M. Koppenhöfer, Rutgers University, New Brunswick, NJ, for comments on an earlier version of the manuscript and Adnan Menderes University for support of this study.

References

  1. Abbott WS (1925) A method for computing the effectiveness of an insecticide. J Econ Entomol 18:265–267CrossRefGoogle Scholar
  2. Akhurst RJ (1983) Neoaplectana species: specificity of association with bacteria of the genus Xenorhabdus. Int J Syst Bacteriol 33:38–45CrossRefGoogle Scholar
  3. Alatorre-Rosas R, Kaya HK (1990) Interspecific competition between entomopathogenic nematodes in the genera Heterorhabditis and Steinernema for an insect host in sand. J Invertebr Pathol 55:179–188CrossRefGoogle Scholar
  4. Anonymous. 2008. T.C. Tarim ve Koyisleri Bakanligi Tarimsal Arastirmalar Genel Mudurlugu, Zirai Mucadele Teknik Talimatlari. Cilt 4, Ankara, 316–318Google Scholar
  5. Ansari MA, Waeyenberge L, Moens M (2005) First record of Steinernema glaseri Steiner, 1929 (Rhabditida: Steinernematidae) from Belgium: a natural pathogen of Hoplia philantus (Coleoptera: Scarabeidae). Nematology 7:953–956CrossRefGoogle Scholar
  6. Avtzis DN, Cognato AI (2013) Genetic structure of Curculio elephas Gyll. (Coleoptera, Curculionidae) in Greece: an important pest of sweet chestnut. J Pest Sci 86:491–497CrossRefGoogle Scholar
  7. Barbercheck ME, Kaya HK (1990) Interactions between Beauveria bassiana and the entomogenous nematodes Steinernema feltiae and Heterorhabditis heliothidis. J Invertebr Pathol 55:225–234CrossRefGoogle Scholar
  8. Campbell JF, Lewis EE, Stock SP, Nadler S, Kaya HK (2003) Evolution of host search strategies in entomopathogenic nematodes. J Nematol 35:142–145PubMedCentralPubMedGoogle Scholar
  9. Choo HY, Kaya HK, Shea P, Noffsinger EM (1987) Ecological study of nematode parasitism in Ips beetles from California and Idaho. J Nematol 19:495–502PubMedCentralPubMedGoogle Scholar
  10. Choo HY, Koppenhöfer AM, Kaya HK (1996) Combination of two entomopathogenic nematode species for suppression of an insect pest. J Econ Entomol 89:97–103CrossRefGoogle Scholar
  11. Choo HY, Kaya HK, Huh J, Lee DW, Kim HH, Lee SM, Choo YM (2002) Entomopathogenic nematodes (Steinernema spp. and Heterorhabditis bacteriophora) and a fungus Beauveria brongniartii for biological control of the white grubs, Ectinohoplia rufipes and Exomala orientalis, in Korean golf courses. Biocontrol 47:177–192CrossRefGoogle Scholar
  12. Desouhant E (1998) Selection of fruits for oviposition by the chestnut weevil, Curculio elephas. Entomol Exp Appl 86:71–78CrossRefGoogle Scholar
  13. Dunphy GB, Rutherford TA, Webster JM (1985) Growth and virulence of Steinernema glaseri influenced by different subspecies of Xenorhabdus nematophilus. J Nematol 17:476–482PubMedCentralPubMedGoogle Scholar
  14. Ertan E, Seferoglu G (2003) The comparison of the biochemical characteristics of chestnut at fruit ripening and after traditional storage periods. Biosci Res Bull 19:139–149Google Scholar
  15. Gaugler R, Wang Y, Campbell JF (1994) Aggressive and evasive behaviors in Popillia japonica (Coleoptera: Scarabaeidae) larvae: defenses against entomopathogenic nematode attack. J Invertebr Pathol 64:193–199CrossRefGoogle Scholar
  16. Gulcu B, Hazir S (2012) An alternative storage method for entomopathogenic nematodes. Turk J Zool 36:562–565Google Scholar
  17. Han RC, Ehlers RU (2000) Pathogenicity, development, and reproduction of Heterorhabditis bacteriophora and Steinernema carpocapsae under axenic in vivo conditions. J Invertebr Pathol 75:55–58CrossRefPubMedGoogle Scholar
  18. Hazir S, Kaya HK, Stock SP, Keskin N (2003) Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) for biological control of soil pests. Turk J Biol 27:181–202Google Scholar
  19. Karagoz, M., Gulcu, B. and Hazir, S. 2007. Manas larvalarının (Coleoptera: Scarabeidae) biyolojik mücadelesinde entomopatojenik nematodların test edilmesi. I. Entomopatojenler ve Mikrobiyal Mücadele Sempozyumu, 21–24 Haziran, TrabzonGoogle Scholar
  20. Karagoz M, Gulcu B, Hazir S, Kaya HK (2009) Laboratory evaluation of Turkish entomopathogenic nematodes for suppression of the chestnut pests, Curculio elephas (Coleoptera: Curculionidae) and Cydia splendana (Lepidoptera: Tortricidae). Biocontrol Sci Techn 19:755–768CrossRefGoogle Scholar
  21. Karimi, J., Rezapanah, M., Monfared, F. and Mirsaeidi, H. 2010. Biological control potential of an entomopathogenic preparation of Heterorhabditis bacteriophora on the white grub Polyphylla adspersa. Society for Invertebrate Pathology meeting, 11–15 July, Trabzon TurkeyGoogle Scholar
  22. Kaya HK (1984) Nematode parasites of bark beetle. In: Nickle WR (ed) Plant and insect nematodes. Marcel Dekker, New York, pp 727–754Google Scholar
  23. Kaya HK, Gaugler R (1993) Entomopathogenic nematodes. Annu Rev Entomol 38:181–206CrossRefGoogle Scholar
  24. Kaya HK, Stock SP (1997) Techniques in insect nematology. In: Lacey LA (ed) Manual of techniques in ınsect pathology. Academic, London, pp 281–324CrossRefGoogle Scholar
  25. Kepenekci I, Gokce A, Gaugler R (2004) Virulence of three species of entomopathogenic nematodes to the chestnut weevil, Curculio elephas (Coleoptera: Curculionidae). Nematropica 34:199–204Google Scholar
  26. Kondo E (1989) Studies on the infectivity and propagation of entomogenous nematodes, Steinernema spp. (Rhabditida: Steinernematidae) in the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae). Bull Fac Agric 67:1–87Google Scholar
  27. Koppenhöfer AM, Grewal PS (2005) Compatibility and interactions with agrochemicals and other biological control agents. In: Grewal PS, Ehlers RU, Shapiro-Ilan DI (eds) Nematodes as biocontrol agents. CABI, Wallingford, pp 363–381CrossRefGoogle Scholar
  28. Koppenhöfer AM, Kaya HK (1997) Additive and synergistic interactions between entomopathogenic nematodes and Bacillus thuringiensis for scarab grub control. Biol Control 8:131–137CrossRefGoogle Scholar
  29. Koppenhöfer AM, Kaya HK, Shanmugan S, Wood GL (1995) Interspecific competition between steinernematid nematodes within an insect host. J Invertebr Pathol 66:99–103CrossRefGoogle Scholar
  30. Koppenhöfer AM, Choo HY, Kaya HK, Lee DW, Gelernter WD (1999) Increased field and greenhouse efficacy against scarab grubs with a combination of an entomopathogenic nematode and Bacillus thuringiensis. Biol Control 14:37–44CrossRefGoogle Scholar
  31. Koppenhöfer AM, Grewal PS, Kaya HK (2000) Synergism of imidacloprid and entomopathogenic nematodes against white grubs: the mechanism. Entomol Exp Appl 94:283–293CrossRefGoogle Scholar
  32. Sankar M, Prasad JS, Padmakumari AP, Katti G, Divya K (2009) Combined application of two entomopathogenic nematodes, Heterorhabditis indica and Steinernema asiaticum to control the rice leaf folder, Cnaphalocrosis medinalis (Goen.). J Biopestic 2:135–140Google Scholar
  33. Shapiro-Ilan DI, Jackson M, Reilly CC, Hotchkiss MW (2004) Effects of combining an entomopathogenic fungi or bacterium with entomopathogenic nematodes on mortality of Curculio caryae (Coleoptera: Curculionidae). Biol Control 30:119–126CrossRefGoogle Scholar
  34. Shapiro-Ilan DI, Cottrell TE, Wood BW (2011) Effects of combining microbial and chemical insecticides on mortality of the pecan weevil (Coleoptera: Curculionidae). J Econ Entomol 104:14–20CrossRefPubMedGoogle Scholar
  35. Speranza S (1999) Chestnut pests in central Italy. Acta Hort 494:417–423Google Scholar
  36. SPSS. 2004. SPSS v.13.0 for windows. SPSS, ChicagoGoogle Scholar
  37. Thurston GS, Kaya HK, Burlando TM, Harrison RE (1993) Milky disease bacterium as a stressor to increase susceptibility of scarabaeid larvae to an entomopathogenic nematode. J Invertebr Pathol 61:167–172CrossRefGoogle Scholar
  38. Thurston GS, Kaya HK, Gaugler R (1994) Characterizing the enhanced susceptibility of milky disease-infected scarabaeid grubs to entomopathogenic nematodes. Biol Control 4:67–73CrossRefGoogle Scholar
  39. Unlu I, Ehlers R-U, Susurluk A (2007) Additional data and first record of the entomopathogenic nematode Steinernema weiseri from Turkey. Nematology 9:739–741CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Sevdiye Demir
    • 1
  • Mehmet Karagoz
    • 1
  • Selcuk Hazir
    • 2
    Email author
  • Harry K. Kaya
    • 3
  1. 1.Department of Plant Protection, Faculty of AgricultureAdnan Menderes UniversityAydinTurkey
  2. 2.Department of Biology, Faculty of Arts and SciencesAdnan Menderes UniversityAydinTurkey
  3. 3.Nematology DepartmentUniversity of CaliforniaDavisUSA

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