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Journal of Pest Science

, Volume 88, Issue 1, pp 209–218 | Cite as

Herbicidal activity of eugenol towards some grassy and broad-leaved weeds

  • Nitina Ahuja
  • Daizy R. Batish
  • Harminder Pal Singh
  • Ravinder K. Kohli
Original Paper

Abstract

The present study investigated the phytotoxic potential of eugenol, a major component from the essential oil of clove [Syzygium aromaticum (L.) Merrill and Perry], towards four grassy [Echinochloa crus-galli (L.) Beauv., Phalaris minor Retz., Sorghum halepense (L.) Pers. and Leptochloa chinensis (L.) Nees] and four broad-leaved [Ageratum conyziodes L., Commelina benghalensis L., Cassia occidentalis L. and Bidens pilosa L.] weeds. The effect of eugenol (50–1,000 μM) on the growth and development of seedlings after 7 days of treatment was studied in terms of percent germination, root and shoot length, total chlorophyll content and cellular respiration. Eugenol at 1,000 μM caused 55–70 and 42–90 % decrease in percent germination in grassy and broad-leaved weeds, respectively. Likewise, root length declined by 55–90 and 57–71 %, whereas shoot length was decreased by 50–83 and 36–73 % in grassy and broad-leaved weeds, respectively, in response to 1,000 μM eugenol. The observed reduction in the plant growth was accompanied by a decline in the total chlorophyll content (37–53 %) and cellular respiration (36–57 %) in the test plants. However, the inhibitory effect was stronger towards grassy weeds than towards the broad-leaved ones. Thus, future research can be focused on developing eugenol, a natural plant product, as an environmentally safe herbicide in replacement to the harmful chemical herbicides.

Keywords

Eugenol Monoterpene Phytotoxicity Dose–response bioassay Bioherbicide 

Notes

Acknowledgments

N. Ahuja is thankful to the University Grants Commission, India, for the financial support in the form of fellowship.

References

  1. Abrahim D, Braguini WL, Kelmer-Bracht AM, Ishii-Iwamoto EL (2000) Effects of four monoterpenes on germination, primary growth, and mitochondrial respiration of maize. J Chem Ecol 26:611–623CrossRefGoogle Scholar
  2. Amri I, Lamia H, Mohsen H, Baseem J (2012) Herbicidal potential of essential oils from three Mediterranean trees on different weeds. Curr Bioact Compd 8:3–12CrossRefGoogle Scholar
  3. Arnon DI (1949) Copper enzymes in isolated chloroplasts: polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15CrossRefPubMedCentralPubMedGoogle Scholar
  4. Bainard LD, Isman MB, Upadhyaya MK (2006) Phytotoxicity of clove oil and its primary constituent eugenol and the role of leaf epicuticular wax in the susceptibility to these essential oils. Weed Sci 54:833–837CrossRefGoogle Scholar
  5. Batish DR, Singh HP, Setia N, Kohli RK, Yadav SS (2007) Alternative control of little seed canary grass using eucalypt oil. Agron Sustain Dev 27:171–177CrossRefGoogle Scholar
  6. Batish DR, Singh HP, Kohli RK, Kaur S (2008) Eucalyptus essential oil as natural pesticide. For Ecol Manag 256:2166–2174CrossRefGoogle Scholar
  7. Boyd NS, Brennan EB (2006) Burning nettle, common purslane, and rye response to a clove oil herbicide. Weed Technol 20:646–650CrossRefGoogle Scholar
  8. Dayan FE, Cantrell CL, Duke SO (2009) Natural products in crop protection. Bioorg Med Chem 17:4024–4034Google Scholar
  9. De Martino L, Mancini E, De Almeda LFR, De Feo V (2010) The antigerminative activity of twenty seven monoterpenes. Molecules 15:6630–6637CrossRefPubMedGoogle Scholar
  10. del Moral R, Muller CH (1970) The allelopathic effects of Eucalyptus camaldulensis. Am Midl Nat 83:254–282CrossRefGoogle Scholar
  11. Dudareva N, Negre F, Nagegowda AD, Orlova I (2006) Plant volatiles: recent advances and future perspectives. Crit Rev Plant Sci 25:417–440CrossRefGoogle Scholar
  12. Duke SO, Dayan FE, Romagni JG, Rimando AM (2000) Natural products as sources of herbicides: current status and future trends. Weed Res 40:99–111CrossRefGoogle Scholar
  13. Evans GJ, Bellinder RR, Goffinet MC (2009) Herbicidal effects of vinegar and a clove oil product on redroot pigweed (Amaranthus retroflexus) and velvetleaf (Abutilon theophrasti). Weed Technol 23(2):292–299CrossRefGoogle Scholar
  14. Ghnaya AB, Hanana M, Amri I, Balti H, Gargouri S, Jamoussi B, Hamrouni L (2013) Chemical composition of Eucalyptus erythrocorys essential oils and evaluation of their herbicidal and antifungal activities. J Pest Sci 86:571–577CrossRefGoogle Scholar
  15. Hiscox JD, Israelstam GF (1979) A method for extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334CrossRefGoogle Scholar
  16. Hsiung YC, Chen YA, Chen SY, Chi WC, Lee RH, Chiang TY, Huang HJ (2013) Volatile myrcene inhibits growth and activates defense responses in rice roots. Acta Physiol Plant 35:2475–2482CrossRefGoogle Scholar
  17. Ishii-Iwamoto EL, Pergo EM, Reis B, Moscheta IS, Bonato CM (2012) Effects of monoterpenes on physiological processes during seed germination and seedling growth. Curr Bioact Compd 8:50–64CrossRefGoogle Scholar
  18. Isman MB (2000) Plant essential oils for pest and disease management. Crop Prot 19:603–608CrossRefGoogle Scholar
  19. Kamatou GP, Vermaak I, Viljoen AM (2012) Eugenol: from the remote Maluku islands to the international market place: a review of a remarkable and versatile molecule. Molecules 17:6953–6981CrossRefPubMedGoogle Scholar
  20. Kaur S, Singh HP, Mittal S, Batish DR, Kohli RK (2010) Phytotoxic effects of volatile oil from Artemisia scoparia against weeds and its possible use as a bioherbicide. Ind Crops Prod 32:54–61CrossRefGoogle Scholar
  21. Kaur S, Singh HP, Batish DR, Kohli RK (2011) Chemical characterization and allelopathic potential of volatile oil of Eucalyptus tereticornis against Amaranthus viridis. J Plant Int 6:297–302Google Scholar
  22. Kordali S, Cakir A, Sutay S (2007) Inhibitory effects of monoterpenes on seed germination and seedling growth. Z Naturforsch 62:207–214Google Scholar
  23. Liu HJ, Chai Q, Huang GB, Zhou HV, Zhu J (2013) Effects of eugenol on soil microbes in wheat faba-bean intercropping and mono-cropping systems. Pratacult Sci 30:346–351Google Scholar
  24. Maness P-C, Smolinski S, Blake DM, Huang Z, Wolfrum EJ, Jacoby WA (1999) Bactericidal activity of photocatalytic TiO2 reaction: toward an understanding of its killing mechanism. Appl Environ Microb 65:4094–4098Google Scholar
  25. Meyer SLF, Lakshman DK, Zasada IA, Vinyard BT, Chitwood DJ (2008) Dose-response effects of clove oil from Syzygium aromaticum on the rootknot nematode Meloidogyne incognita. Pest Manag Sci 64:223–229CrossRefPubMedGoogle Scholar
  26. Muller WH, Muller CH (1964) Volatile growth inhibitors produced by Salvia species. Bull Torrey Bot Club 91:327–330CrossRefGoogle Scholar
  27. Mutlu S, Atici O, Esim N (2010) Bioherbicidal effects of the essential oils of Nepeta meyeri Benth. on weed spp. Allelopath J 26:291–300Google Scholar
  28. Mutlu S, Atici O, Esim N, Mete E (2011) Essential oils of catmint (Nepeta meyeri Benth.) induce oxidative stress in early seedlings of various weeds species. Acta Physiol Plant 33(3):943–951CrossRefGoogle Scholar
  29. Nishida N, Tamotsu S, Nagata N, Saito C, Sakai A (2005) Allelopathic effects of volatile monoterpenoids produced by Salvia leucophylla: inhibition of cell proliferation and DNA synthesis in the root apical meristem of Brassica campestris seedlings. J Chem Ecol 31:1187–1203CrossRefPubMedGoogle Scholar
  30. Rabenhorst J (1996) Production of methoxyphenol-type natural aroma chemicals by biotransformation of eugenol with a new Pseudomonas sp. Appl Microbiol Biotechnol 46:470–474CrossRefGoogle Scholar
  31. Rani D, Kohli RK (1991) Fresh matter is not an appropriate relation unit for chlorophyll content: experience from experiments on effects of herbicides and allelopathic substances. Photosynthetica 25:655–658Google Scholar
  32. Romagni JG, Allen SN, Dayan FE (2000) Allelopathic effects of volatile cineoles on two weedy plant species. J Chem Ecol 26:303–313CrossRefGoogle Scholar
  33. Singh HP, Batish DR, Kaur S, Ramezani H, Kohli RK (2002a) Comparative phytotoxicity of four monoterpenes against Cassia occidentalis. Ann Appl Biol 141:111–116CrossRefGoogle Scholar
  34. Singh HP, Batish DR, Kohli RK (2002b) Allelopathic effect of two volatile monoterpenes against billgoat weed (Ageratum conyzoides L.). Crop Prot 21:347–350CrossRefGoogle Scholar
  35. Singh HP, Batish DR, Kohli RK (2003) Allelopathic interactions and allelochemicals: new possibilities for sustainable weed management. Crit Rev Plant Sci 22:239–311CrossRefGoogle Scholar
  36. Singh HP, Batish DR, Kaur S, Kohli RK, Arora K (2006) Phytotoxicity of the volatile monoterpene citronellal against some weeds. Z Naturforsch 61:334–340Google Scholar
  37. Stokłosa A, Matraszek R, Isman MB, Upadhyaya MK (2012) Phytotoxic activity of clove oil, its constituents, and its modification by light intensity in broccoli and common lambsquarters (Chenopodium album). Weed Sci 60:607–611CrossRefGoogle Scholar
  38. Stroh J, Wan MT, Isman MB, Moul DJ (1998) Evaluation of the acute toxicity to juvenile pacific coho salmon and rainbow trout of some plant essential oils, a formulated product, and the carrier. Bull Environ Contam Toxicol 60:923–930CrossRefPubMedGoogle Scholar
  39. Tworkoski T (2002) Herbicide effects of essential oils. Weed Sci 50:425–431CrossRefGoogle Scholar
  40. Vasilakoglou I, Dhima K, Paschalidis K, Ritzoulis C (2013) Herbicidal potential on Lolium rigidum of nineteen major essential oil components and their synergy. J Essent Oil Res 25:1–10CrossRefGoogle Scholar
  41. Vaughn SF, Spencer GF (1993) Volatile monoterpenes as potential parent structures for new herbicides. Weed Sci 41:114–119Google Scholar
  42. Weidenhamer JD, Macias FA, Fischer NH, Williamson GB (1993) Just how insoluble are monoterpenes? J Chem Ecol 19:1799–1807CrossRefPubMedGoogle Scholar
  43. Yoshimura H, Sawaj Y, Tomatsu S, Sakai A (2011) 1,8-cineole inhibits both proliferation and elongation of BY-2 culture tobacco cells. J Chem Ecol 37:320–328CrossRefPubMedCentralPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Nitina Ahuja
    • 1
  • Daizy R. Batish
    • 1
  • Harminder Pal Singh
    • 2
  • Ravinder K. Kohli
    • 1
  1. 1.Department of BotanyPanjab UniversityChandigarhIndia
  2. 2.Department of Environment StudiesPanjab UniversityChandigarhIndia

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