Journal of Pest Science

, Volume 83, Issue 4, pp 461–470 | Cite as

Relative efficacy of strobilurin formulations in the control of downy mildew of sunflower

  • J. Sudisha
  • S. R. NiranjanaEmail author
  • S. L. Sukanya
  • R. Girijamba
  • N. Lakshmi Devi
  • H. Shekar Shetty


The efficacy of three commercial formulations of strobilurins, viz., trifloxystrobin, kresoxim-methyl, and azoxystrobin was evaluated against sunflower downy mildew disease caused by Plasmopara halstedii under laboratory, greenhouse, and field conditions. Complete inhibition of sporangial sporulation, zoospore release, and motility was observed with 2 μg ml−1 in trifloxystrobin, and 5 μg ml−1 for azoxystrobin and kresoxim-methyl. Seed treatment with different concentrations of strobilurins enhanced seed germination and seedling vigor of sunflower to varying degrees compared to control. Highest seed germination was recorded at 10 μg ml−1 in kresoxim-methyl, and maximum seedling vigor was noticed with trifloxystrobin at 30 μg ml−1. The effect of strobilurins was tested as seed treatment, foliar application, and seed treatment followed by foliar application. Under greenhouse conditions none of the concentrations used, either as seed treatment and foliar application, were phytotoxic. For the three strobilurins, the seed treatment along with foliar application enhanced the protection of the plants as compared to only the treatment of seeds. Foliar spray treatments alone provided an intermediate control of the disease. Trifloxystrobin showed a better effect than kresoxim-methyl and azoxystrobin. Disease curative activity of trifloxystrobin was higher compared to kresoxim-methyl and azoxystrobin. Tested fungicides when applied on adaxial leaf surface showed partial translaminar activity, and disease inhibition was marginal. Loss of trifloxystrobin and azoxystrobin activity over time was low, indicating stable rainfastness residual activity. A trend in protection against downy mildew disease similar to greenhouse results was evident in the field trials. This is the first report of strobilurins exhibiting high activity against P. halstedii and is a promising fungicide for controlling sunflower downy mildew disease by seed treatment and foliar spray.


Helianthus annuus L. Plasmopara halstedii β methoxyacrylic acid group formulations Seed and foliar treatments Disease management 



The authors thank Dr. T. S. Thind, Professor, Department of Plant Pathology, Punjab Agricultural University, Ludhiana, Punjab, India for supplying the experimental fungicides and Indian Council of Agricultural Research (ICAR) through All India Co-ordinated Pearl Millet Improvement Project (AICPMIP), Government of India for laboratory and field facilities provided at University of Mysore.


  1. Abdul Baki AA, Anderson JD (1973) Vigor determination in soybean seed by multiple criteria. Crop Sci 13:630–633CrossRefGoogle Scholar
  2. Agrawal SC, Gupta RK, Prasad KVV (1991) A case of downy mildew of sunflower in Madhya Pradesh. J Oilseeds Res 8:126Google Scholar
  3. Albourie JM, Tourvieille J, de Tourvieille Labrouhe D (1998) Resistance to metalaxyl in isolates of the sunflower pathogen Plasmopara halstedii. Eur J Plant Pathol 104:235–242CrossRefGoogle Scholar
  4. Anke T, Oberwinkler F, Steglich W, Schramm G (1977) The strobilurins new antifungal antibiotics from the basidiomycete Strobiluris tenacellus. J Antibiotics 30:806–810CrossRefGoogle Scholar
  5. Anonymous (1993) International rules for seed testing. Seed Sci Technol 13:309–333Google Scholar
  6. Attard A, Gourgues M, Galiana E, Panabieres F, Ponchet M, Keller H (2007) Strategies of attack and defense in plant oomycete interactions, accentuated for Phytophthora parasitica dastur (syn. P. nicotianae Breda de Haan). J Plant Physiol. doi: 10.1016/j.jplph.2007.06.011
  7. Bartlett DW, Clough JM, Godwin JR, Hall AA, Hamer M, Parr-Dobrzanski B (2002) The strobilurin fungicides. Pest Manag Sci 58:649–662CrossRefPubMedGoogle Scholar
  8. Beck C, Oerke EC, Dehne HW (2002) Impact of strobilurins on physiology and yield formation of wheat. Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 67(2):181–187PubMedGoogle Scholar
  9. Cohen Y, Sackston WE (1973) Factors affecting infection of sunflowers by Plasmopara halstedii. Can J Bot 51:15–22CrossRefGoogle Scholar
  10. Davidse LC, van den Berg-Velthuis GCM, Mantel BC, Jespers ABK (1991) Phenylamides and Phytophthora. In: Lucas JA, Shattock RC, Shaw DS, Cooke LR (eds) Phytophthora. British Mycological Society, Cambridge, pp 349–360Google Scholar
  11. Farber RB, Chin KM, Leadbitter N (2002) Sensitivity of Venturia inaequalis to trifloxystrobin. Pest Manag Sci 58(3):261–267CrossRefPubMedGoogle Scholar
  12. Gisi U, Chin KM, Knapova G, Kung Farber R, Mohr U, Parisi S, Sierotzki H, Steinfeld U (2000) Recent developments in elucidating modes of resistance to phenylamide, DMI and strobirulin fungicides. Crop Protection 19:863–872CrossRefGoogle Scholar
  13. Gulya TJ, Draper M, Harbour J, Holen C, Knodel J, Lamey A, Mason P (1999) Metalaxyl resistance in sunflower downy mildew in North America. Proc. 21st Sunf. Res. Workshop, Jan 14 & 15, 1999, Fargo ND, USA, pp 2–7Google Scholar
  14. Hermann D, Fischer W, Knauf-Baider G, Steinemann A, Margot P, Gisi U, Laird D (1998) Behavior of the new strobilurin fungicide trifloxystrobin on and in plants. Phytopathology 88:S37Google Scholar
  15. Herms S, Seehaus K, Koehle H, Conrath U (2003) A strobilurin fungicide enhances the resistance of tobacco against tobacco mosaic virus and Pseudomonas syringae pv tabaci. Plant Physiol 130:120–127CrossRefGoogle Scholar
  16. Karadimos DA, Karaoglanidis GS, Klonari TK (2005) Biological activity and physical modes of action of the Qo inhibitor fungicides trifloxystrobin and pyraclostrobin against Cercospora beticola. Crop Protection 24:23–29CrossRefGoogle Scholar
  17. Lamour KH, Hausbeck MK (2000) Mefenoxam insensitivity and the sexual stage of Phytophthora capsici in Michigan cucurbit fields. Phytopathology 90:396–400CrossRefPubMedGoogle Scholar
  18. Margot P, Huggenberger F, Amrein J, Weiss B (1998) A new broad-spectrum strobilurin fungicide. Brighton Crop Prot Conf Pest Disease 2:375–382Google Scholar
  19. Matheron ME, Porchas M (2000) Impact of azoxystrobin, dimethomorph, fluazinam and fosetyl-Al and metalaxyl on growth, sporulation and zoospore cyst germination of three Phytophthora sp. Plant Dis 84:454–458CrossRefGoogle Scholar
  20. Mayee CD, Patil MA (1986) Downy mildew of sunflower. Indian Pytopathol 39:314Google Scholar
  21. Melero-vara JM, Garcia-Baudin C, Lopez-Herrera CJ, Jimenez-Diaz RM (1982) Control of sunflower downy mildew with metalaxyl. Plant Dis 66:132–135CrossRefGoogle Scholar
  22. Mitani S, Araki S, Takii Y, Ohisma T, Matsuo N, Miyoshi H (2001) The biochemical mode of action of the novel selective fungicide cyazofamid: specific inhibition of mitochondrial complex III in Pythium spinosum. Pestic Biochem Physiol 71:107–115CrossRefGoogle Scholar
  23. Molinero-Ruiz ML, Domínguez J, Melero-Vara JM (2002) Races of isolates of Plasmopara halstedii from Spain and studies on their virulence. Plant Dis 86:736–740CrossRefGoogle Scholar
  24. Molinero-Ruiz ML, Melero-Vara JM, Gulya TJ, Dominguez J (2003) First report of resistance to metalaxyl in downy mildew of sunflower caused by Plasmopara halstedii in Spain. Plant Dis 87:749CrossRefGoogle Scholar
  25. Molinero-Ruiz ML, Dominguez J, Gulya TJ, Melero-Vara JM (2005) Reaction of field populations of sunflower downy mildew (Plasmopara halstedii) to metalaxyl and mefenoxam. Helia 28:65–74CrossRefGoogle Scholar
  26. Molinero-Ruiz ML, Cordon-Torres MM, Melero-Vara JM, Dominguez J (2008) Resistance to metalaxyl and to metalaxyl-M in populations of Plasmopara halstedii causing sunflower downy mildew. Can J Plant Pathol 30:97–105CrossRefGoogle Scholar
  27. Oros G, Viranyi F (1984) Resistance of Plasmopara halstedii to metalaxyl in the greenhouse. Temperate Downy Mildew Newsletter 3:22–23Google Scholar
  28. Parra G, Ristaino JB (1998) Insensitivity to Ridomil Gold (mefenoxam) found among field isolates of Phytophthora capsici causing Phytophthora blight on bell pepper in North Carolina and New Jersey. Plant Dis 82:711CrossRefGoogle Scholar
  29. Parra G, Ristaino JB (2001) Resistance to mefenoxam and metalaxyl among field isolates of Phytophthora capsici causing Phytophthora blight of bell pepper. Plant Dis 85:1069–1075CrossRefGoogle Scholar
  30. Patil MA, Phad H, Ramtirthkar MS, Phad HB (1991) Chemical control of sunflower downy mildew caused by Plasmopara halstedii. Indian Phytopathol 44:325–327Google Scholar
  31. Reuveni M (2001) Activity of trifloxystrobin against powdery and downy mildew diseases of grapevines. Can J Plant Pathol 23:52–59CrossRefGoogle Scholar
  32. Sauter H, Steglich W, Anke T (1999) Strobilurins: evolution of new class of active substances. Angew Chem Int Ed 38:1328–1349CrossRefGoogle Scholar
  33. Shetty K (1998) Managing metalaxyl-resistance in Plasmopara halstedii from a chemical company perspective. Symposium III, Sunflower Downy Mildew, Jan 13 & 14, 1998, Fargo ND, USA, pp 74–78Google Scholar
  34. Stenzel K, Pontzen R, Seitz T, Tiemann R, Witzenberger A (1998) SZX 722: a novel systemic oomycete fungicide. Proc Brighton Crop Prot Conf—Pests and Diseases, BCPC, Farnham, Surrey, UK, pp 361–374Google Scholar
  35. Sudisha J, Amruthesh KN, Deepak SA, Shetty NP, Sarosh BR, Shetty HS (2005) Comparative efficacy of strobilurin fungicides against downy mildew disease of pearl millet. Pesticide Biochem Physiol 81:188–197CrossRefGoogle Scholar
  36. Sukul P, Spiteller M (2000) Metalaxyl: persistence, degradation, metabolism and analytical methods. Rev envir Contam toxicol 164:1–26Google Scholar
  37. Viranyi F, Gulya TJ, Masirevic S (1992) Races of Plasmopara halstedii in Central Europe and their metalaxyl sensitivity. Proc 13th Int Sunf Conf, Vol I, Sept 7–11, 1992, Pisa, Italy, pp 865–868Google Scholar
  38. Wiggins TE, Jager BJ (1993) Mode of action of the new methoxyacrylate antifungal agent ICIA5504. Biochem Soc Trans 22:68SCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • J. Sudisha
    • 1
  • S. R. Niranjana
    • 1
    Email author
  • S. L. Sukanya
    • 1
  • R. Girijamba
    • 1
  • N. Lakshmi Devi
    • 2
  • H. Shekar Shetty
    • 1
  1. 1.Downy Mildew Research laboratory, Department of Studies in BiotechnologyUniversity of Mysore, ManasagangotriMysoreIndia
  2. 2.Department of Studies in MicrobiologyUniversity of Mysore, ManasagangotriMysoreIndia

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