Journal of Pest Science

, Volume 83, Issue 1, pp 27–32 | Cite as

Effect of botanical insecticide Nimbecidine® on food consumption and egg hatchability of the terrestrial snail Monacha obstructa

  • Maha A. Shoaib
  • Mahmoud F. Mahmoud
  • Nagla Loutfy
  • Mohamed A. Tawfic
  • Marek BartaEmail author
Original Paper


In the laboratory, a commercial neem-based insecticide—Nimbecidine®—was evaluated as a potential pest management tool for the terrestrial snail, Monacha obstructa (Pfeiffer, 1842) (Hygromiidae). Effects of different concentrations of the botanical insecticide on food consumption and egg hatchability of the terrestrial snail were studied. Generally, food consumption of immature and adult snails decreased as the concentrations of Nimbecidine® increased. At the highest concentration (10 ml/l), the snails avoided contacting with food completely. The food intake of immature individuals was significantly (p < 0.05) more affected by the Nimbecidine® treatment (at 1.25 ml/l) than that of adults. LC50 of Nimbecidine® for the treated eggs was 2.18 ml/l, and eggs failed to hatch at concentration of 10 ml/l. Nimbecidine® showed sufficient biological activity against the food consumption and eggs viability of M. obstructa, thus the preparation has a potential to protect field crops from this pest snails.


Neem oil Antifeedant effect Ovicidal effect Hygromiidae Monacha obstructa 



The authors thank T. Stanes & Company Ltd, Coimbatore, India for providing us with a free sample of Nimbecidine® for this research work.


  1. Bhatnagar D, Zeringue HJ, McCormick SP (1990) Neem leaf extracts inhibit aflatoxin biosynthesis in Aspergillus flavus and A. parasiticus. In: Locke JC, Lawson RH (eds) Proceedings of a workshop on neem’s potential in pest management programs. USDA-ARS, Beltsville, pp 118–127Google Scholar
  2. Forster P (1991) EinfluB Von Pflanzenschutzmitteln auf-larven and Adulte Von Platymus dorsalis (Pont) (Coleoptera: Carabidae) Und auf Adulte Von Tachyporus hyphorum (L.) (Coleoptera: Staphylinidae) in Labor-Halbfreiland Und Freilandversuchen. Doctoral thesis, University of Giessen, GermanyGoogle Scholar
  3. Gabr WM, Youssef AS, Khidr FK (2006) Molluscicidal effect of certain compounds against two land snail species, Monacha obstructa and Eobania vermiculata under laboratory and field conditions. Egypt J Agric Res 84:43–50Google Scholar
  4. Garthwaite DG, Thomas MR (1996) The usage of molluscicides in agriculture and horticulture in Great Britain over the last 30 years. In: Henderson IF, Fanham UK (eds) Slugs and snail’s pests in agriculture. British Crop Protection Council, Symposium Proceedings No 66, pp 39–46Google Scholar
  5. Hollingsworth RG, Armstrong JW (2003) Effectiveness of products containing metaldehyde, copper or extracts of yucca or neem for control of Zonitoides arboreus (Say), a snail pest of orchid roots in Hawaii. Int J Pest Manag 49:115–122CrossRefGoogle Scholar
  6. Iglesias J, Castillejo J, Ester A (2002a) Laboratory evaluation of potential molluscicides for the control of eggs of the pest slug Deroceras reticulatum (Müller) (Pulmonata: Limacidae). Int J Pest Manag 48:19–23CrossRefGoogle Scholar
  7. Iglesias J, Castillejo J, Ester A, Castro R, Lombardia MJ (2002b) Susceptibility of the eggs of the field slug Deroceras reticulatum to contact with pesticides and substances of biological origin on artificial soil. Ann Appl Biol 140:53–59CrossRefGoogle Scholar
  8. Ismail SAA, El-Massry SAA, Khattab MM, Hassan AS (2003) Daily activity and damage caused by Eobania vermiculata Muller (Gastropoda: Helicidae) in citrus orchards. Egypt J Appl Sci 18:333–340Google Scholar
  9. Isman MB (1999) Neem and related natural products. In: Hall FR, Menn JJ (eds) Biopesticides: use and delivery. Human, TotowaGoogle Scholar
  10. Jacobson M (1990) Review of neem research in the United States. In: Locke JC, Lawson RH (eds) Proceedings of a workshop on neem’s potential in pest management programs. USDA-ARS, Beltsville, pp 4–14Google Scholar
  11. Jandial VK, Kamlesh M (2008) Evaluation of different insecticides against green peach aphid, Myzus persicae (Sulzer) on potato crop. J Entomol Res 32:49–51Google Scholar
  12. Langan AM, Taylor A, Wheater CP (2004) Effects of metaldehyde and methiocarb on feeding preferences and survival of a slug predator (Pterostichus melanarius (F.): Carabidae, Pterostichini). J Appl Entomol 128:51–55CrossRefGoogle Scholar
  13. Lee SM, Olsen JI, Schweizer MP, Klocke JA (1988) 7-Deacetyl-17β-hydroxyazadiradione, a new limonoid insect growth inhibitor from Azadirachta indica. Phytochemistry 27:2773–2775CrossRefGoogle Scholar
  14. Locke JC (1990) Activity of neem seed oil against fungal plant pathogens. In: Locke JC, Lawson RH (eds) Proceedings of a workshop on neem’s potential in pest management programs. USDA-ARS, Beltsville, pp 132–136Google Scholar
  15. Lowery DT, Isman MB (1995) Toxicity of neem to natural enemies of aphids. Phytoparasitica 23:297–306CrossRefGoogle Scholar
  16. Mohapatra RN, Patnaik NC, Mishra BK, Patnaik HP (1991) Comparative toxicity of some insecticides and neem oil against the brown plant hopper, Nilaparvata lugens (Stal) and its predators. Orissa J Agric Res 4:239–240Google Scholar
  17. Mordue AJ (2004) Present concepts of the mode of action of azadirachtin from neem. In: Koul O, Wahab S (eds) Neem: today and in the new millennium. Kluwer Academic Publishers, Dordrecht, pp 229–242CrossRefGoogle Scholar
  18. Mostafa B, Abdel-Megeed MI (1996) Molluscicidal activity of neem on Biomphalaria alexandrina. Ann Agric Sci (Cairo) Special Issue: 215–232Google Scholar
  19. Nakhla JM, Tadros AW, El-Hwashy N (2002) Monitoring the changes in the population activity of Monacha obstructa in apple orchards and Egyptian clover fields in Giza Governorate. Egypt J Agric Res 80:181–188Google Scholar
  20. National Research Council (1992) Neem: a tree for solving global problems. National Academy Press, Washington, DCGoogle Scholar
  21. Naumann K, Isman MB (1996) Toxicity of neem (Azdirachta indica A. Juss.) seed extracts to larval honeybees and estimation of dangers from field application. Am Bee J 136:518–520Google Scholar
  22. Pal S, Koley KM, Pathak AK (2002) Molluscicidal activity of Azadirachta indica leaves and Lantana camara leaves powders against Indoplanorbis exustus. Indian J Anim Sci 72:561–562Google Scholar
  23. Pallabi D, Saidul I, Manoranjan D, Medhi AK (2007) Ecology and biology of aquatic snails and their control: IV. Phytomolluscicidal effect of nine plant extracts. J Vet Parasitol 21:5–12Google Scholar
  24. Patil RK (2008) Evaluation of biorational pesticides for the management of stem borer, Chilo partellus Swinhoe in sweet sorghum. Karnataka J Agric Sci 21:293–294Google Scholar
  25. Purvis G, Bannon JW (1992) Non-target effects of repeated methiocarb slug pellet application on carabid beetle (Coleoptera: Carabidae) activity in winter-sown cereals. Ann Appl Biol 121:401–422CrossRefGoogle Scholar
  26. Raguraman S, Singh RP (1999) Biological effects of neem (Azadirachta indica) seed oil on eggs parasitoid, Trichogramma chilonis. J Econ Entomol 92:1274–1280Google Scholar
  27. Rao IG, Singh DK (2000) Effect of single and binary combinations of plant-derived molluscicides on reproduction and survival of the snail Achatina fulica. Arch Environ Contam Toxicol 39:486–493CrossRefPubMedGoogle Scholar
  28. Roxburgh W (1874) Description of Indian plants. Today and tomorrow, New Delhi, p 763Google Scholar
  29. Sangita S, Singh VK, Singh DK (2006) The effect of single, binary, and tertiary combination of few plant derived molluscicides alone or in combination with synergist on different enzymes in the nervous tissues of the freshwater snail Lymnaea (Radix) acuminata (Lamark). Pest Biochem Physiol 85:167–173CrossRefGoogle Scholar
  30. SAS Institute Inc. (1999) SAS/STAT® user’s guide. Version 8. Cary, NC. ISBN 1-58025-494-2Google Scholar
  31. Schmutterer H (1990) Properties and potential of natural pesticides from the Neem tree, Azadirachta indica. Annu Rev Entomol 35:271–297CrossRefPubMedGoogle Scholar
  32. Shoieb MA (2008) Occurrence and distribution of terrestrial mollusks in Suez canal governorates and North of Sinai. Egypt J Agric Res 86:25–36Google Scholar
  33. Singh K, Singh DK (2000) Toxicity to the snail Lymnaea acuminata of plant-derived molluscicides in combination with synergists. Pest Manag Sci 56:889–898CrossRefGoogle Scholar
  34. Singh A, Singh DK (2001) Molluscicidal activity of the custard apple (Annona squamosa L.) alone and in combination with other plant derived molluscicides. J Herbs Spices Med Plants 8:23–29CrossRefGoogle Scholar
  35. Singh RS, Singh PS (2007) Effect of bio-rational approaches on pigeonpea pod and grain damage by pod borer [Helicoverpa armigera (Hub.)]. J Food Legumes 20:103–106Google Scholar
  36. Singh K, Singh A, Singh DK (1996) Molluscicidal activity of neem (Azadirachta indica A. Juss). J Ethnopharmacol 52:35–40CrossRefPubMedGoogle Scholar
  37. Singh K, Singh A, Singh DK (1998) The use of piperonyl butoxide and MGK-264 to improve the efficacy of some plant-derived molluscicides. Pestic Sci 58:145–149CrossRefGoogle Scholar
  38. South A (1992) Terrestrial slugs. Biology, ecology and control. Chapman & Hall, London, p 428 Google Scholar
  39. Srinivas K, Madhumathi T (2005) Effect of insecticide applications on the predator population in rice ecosystem of Andhra Pradesh. Pest Manag Econ Zool 13:71–75Google Scholar
  40. Srivastava M, Paul AVN, Rengaswamy S, Kumar J, Parma RBS (1997) Effect of neem (Azadirachta indica A. Juss.) seed kernel extracts on the larval parasitoid, Bracon brevicornis Wesm. (Hym., Braconidae). J Appl Entomol 121:51–57CrossRefGoogle Scholar
  41. Toor RF (2006) The effects of pesticides on Carabidae (Insecta: Coleoptera), predators of slugs (Mollusca: Gastropoda): literature review. N Z Plant Protect 59:208–216Google Scholar
  42. Verkerk RHJ, Wright DJ (1993) Biological activity of neem seed kernel extracts and synthetic azadirachtin against larvae of Plutella xylostella L. Pestic Sci 37:83–91CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Maha A. Shoaib
    • 1
  • Mahmoud F. Mahmoud
    • 1
  • Nagla Loutfy
    • 1
  • Mohamed A. Tawfic
    • 1
  • Marek Barta
    • 2
    Email author
  1. 1.Plant Protection Department, Faculty of AgricultureSues Canal UniversityIsmailiaEgypt
  2. 2.Department of Applied DendrologyArboretum Mlyňany SASSlepčanySlovakia

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