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Journal of Pest Science

, 81:199 | Cite as

Genetic variation and mtCOI phylogeny for Bemisia tabaci (Hemiptera, Aleyrodidae) indicate that the ‘B’ biotype predominates in Iran

  • H. Rajaei ShoorchehEmail author
  • B. Kazemi
  • S. Manzari
  • J. K. Brown
  • A. Sarafrazi
Original Paper

Abstract

Despite a large number of investigations on the molecular genetics and population structure of the whitefly Bemisia tabaci (Gennadius) complex, no such study had been conducted in Iran. The genetic variation of B. tabaci was examined using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for 18 field collections from cucumber, eggplant, and tomato in four provinces of Iran. PCR amplification and restriction digestion with two enzymes detected 388 RFLP fragments, of which 16 fragments showed polymorphisms. Cluster analysis of these data placed all B. tabaci individuals within a single group, and there was no evidence for between- or within-population genetic variation. Phylogenetic (Clustal W) analysis of 42 B. tabaci mtCOI sequences (n = 21 field collections) from Iran, and a comparison with well-studied haplotype or biotype reference sequences available in public sequence databases, revealed that the Iranian B. tabaci populations were most closely related to the B biotype at 0–1.2% nucleotide identity. The B biotype is a well-known member of a sister clade from the Middle East–North African region of the world, owing to its nearly worldwide distribution and invasive characteristics. This report indicates that a single major haplotype of B biotype is prevalent in Iran and that its closest relative is the B biotype. Also, given the extent of known variation in the Middle East and African continent, data indicate somewhat surprisingly that the B. tabaci collections sampled in Iran had limited genetic variation and population substructure. Knowledge that the B biotype of B. tabaci predominates in Iran is important for designing effective pest management strategies given that biotypes of B. tabaci are known to differ greatly with respect to insecticide resistance, host range, virus–vector interactions, and other key biological characteristics.

Keywords

Bemisia tabaci Genetic variation Iran mtCOI PCR-RFLP Phylogeny 

Notes

Acknowledgments

We thank the staff of Insect Taxonomy Research Department, Iranian Research Institute of Plant Protection; Tehran, Iran for kind help, and colleagues of Cellular and Molecular Biology Research Center of Shahid Beheshti University, M.C., Tehran, Iran for useful comments.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • H. Rajaei Shoorcheh
    • 1
    Email author
  • B. Kazemi
    • 2
  • S. Manzari
    • 3
  • J. K. Brown
    • 4
  • A. Sarafrazi
    • 3
  1. 1.Biology DepartmentShahid Beheshti UniversityTehranIran
  2. 2.Cellular and Molecular Biology Research CenterShahid Beheshti UniversityTehranIran
  3. 3.Insect Taxonomy Research DepartmentIranian Research Institute of Plant ProtectionTehranIran
  4. 4.Department of Plant SciencesUniversity of ArizonaTucsonUSA

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