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Thin-Layer Chromatography–Flame Ionization Detection

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Thin layer chromatography–flame ionization detection (TLC–FID) is a versatile analytical technique that can be used for fast analysis of organic compounds. It has been implemented in past decades for the analysis of lipids and petrochemical products, but rarely in other fields. Despite the improvement in the latest Iatroscan model and the introduction of an automatic programmable sample spotter, this system is still struggling to gain acceptance in universities and major research laboratories. The reason behind this might be a lack of awareness on the potential application of this system to other fields of analytical chemistry. This review presents TLC–FID as a mature and reliable, state of the art technique that combines the separation power of TLC with FID as a universal detector, which can be applied to the analysis of a wide variety of organic compounds. Basic operational procedures and previous literature, including its potential for ultrafast analysis of commercial samples, are discussed in order to create awareness on the potentials of this piece of equipment to other fields.

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  1. 1.

    Padley FB (1969) The use of a flame ionization detector to detect components of separated by thin layer chromatography. J Chromatogr 39:37–46

  2. 2.

    Okumura T, Kadono T, Isoo A (1975) Sintered thin-layer chromatography with flame ionization detector scanning. J Chromatogr 108:329–336

  3. 3.

    Crane RT, Goheen SC, Larkin EC, Rao GA (1983) Complexity in lipid quantitation using thin layer chromatography for separation and flame ionization detection. Lipids 18(1):74–80

  4. 4.

    Farnworth ER, Thomson BK, Kramer JKG (1982) Quantitative determination of neutral lipids on chromarods. J Chromatogr 240:463–474

  5. 5.

    Parrish CC, Ackman RG (1983) Chromarod separation for the analysis of marine lipid classes by iatroscan thin-layer chromatography flame ionization detector. J Chromatogr 262:103–112

  6. 6.

    Kramer JKG, Thomson BK, Farnworth R (1986) Variation in the relative response factor for triglycerides on Iatroscan chromarods with fatty acid composition and sequence analysis. J Chromatogr 355:221–228

  7. 7.

    Ackman RG (1981) Flame ionization detection applied to thin-layer chromatography on coated quartz. Methods Enzymol 72:205–252

  8. 8.

    Newman JM (1985) Analysis by Iatroscan TLC-FID system. Lipis 20:501–502

  9. 9.

    Shantha NC (1992) Thin-layer chromatography-flame ionization detection iatroscan system. J Chromatogr 624:21–35

  10. 10.

    Gasparovic B, Kazazic SP, Cvitesic A, Penezic A, Frka S (2015) Improved separation and analysis of glycolipids by iatroscan thin-layer chromatography-flame ionization technique. J Chromatogr A 1409:259–267

  11. 11.

    IP 469, Determination of saturated, aromatic and polar compounds in petroleum products by thin layer chromatography and flame ionization detector, https://publishing.energyinst.org/topics/fuel-quality-and-control/ip-test-methods/ip-469-determination-of-saturated,-aromatic-and-polar-compounds-in-petroleum-products-by-thin-layer-chromatography-and-flame-ionization-detection, Accessed 22 June 2019.

  12. 12.

    Yang BJ, Zheng L, Han XT, Zheng MG (2013) Development of TLC-FID technique for rapid screening of the chemical composition of microalgae diesel and biodiesel blends. Fuel 111:344–349

  13. 13.

    Indrasena WM, Barrow CJ, Kralovec JA (2006) Effect of hydrogen/air flow rates and scan rate on FID response of phospholipids and their qualitative and quantitative analysis by iatroscan (mark-6s) TLC-FID. J Liq Chromatogr Relat Technol 29:211–2127

  14. 14.

    Read H (1985) Improved sample application methods for the iatroscan. Lipids 20:510–515

  15. 15.

    Harvey HR, Rigler MW, Patton JS (1985) The use of the iatroscan to quantify total lipids in a variety of sample type, and lipid classes in human gallbladder. Lipids 20:542–545

  16. 16.

    Parrish CC, Ackman RG (1985) Calibration of the iatroscan-chromarod system of marine lipids classes. Lipids 20:521–530

  17. 17.

    Itoh T, Tanaka M, Kaneko H (1985) Quantitation of lipids and their constituents by chromarods TLC-FID system. Lipids 20:552–554

  18. 18.

    Hazel J (1985) Determination of the phospholipids composition of trout gill by iatroscan TLC-FID. Lipids 20:516–520

  19. 19.

    Penezic A, Gasparovic BZ, Frka S (2010) Distribution of marine classes in salty Rogoznica lake (Croatia). Estuar Coast Shelf Sci 86:625–636

  20. 20.

    Sebedio JL, Farquharson TE, Ackman RG (1985) Quantitative analysis of esters of fatty acids geometric isomers, and triglycerides differing in unsaturation, by Iatroscan TLC-FID technique using silver nitrate impregnated rod. Lipids 20:555–560

  21. 21.

    Patterson PL (1985) Specific detector for nitrogen and halogens compounds in TLC coated with quartz rods. Lipids 20:503–509

  22. 22.

    Parrish CC, Zhou X, Herche L (1988) Flame ionization and flame thermionic detection of carbon and nitrogen in aquatic lipids and humic-type classes with iatroscan mark IV. J Chromatogr 435:350–356

  23. 23.

    Indrasena WM, Ackman RG, Gill TA (1999) Separation of paralytic shellfish poisoning toxins on chromarods-SIII by thin layer chromatography. J Chromatogr A 855:657–668

  24. 24.

    Sinanoglou VJ, Strati IF, Bratakos SM, Proestos C, Zoumpoulakis P, Meimaroglou SM (2013) On the combined application of TLC-FID and GC-FID to identify total, neutral, and polar lipids and their fatty acids extracted from foods, ISRN chromatography, 1–8.

  25. 25.

    ASTM D2007 -16, Standard Test method for characteristic group in rubber extender and processing oils and other petroleum-derived oils by clay-gel adsorption chromatography method, https://www.astm.org/Standards/D2007.htm, Accessed 24 June 2019.

  26. 26.

    ASTM D4124, Standard test method for separation of asphalt into four fractions, https://www.astm.org/Standards/D4124.htm, Accessed 26 June 2019.

  27. 27.

    Selucky ML (1985) Quantitative class separation of coal lipids using thin layer chromatography with flame ionization detection. Lipids 20:546–551

  28. 28.

    Bharati S, Rostum GA, Loberg R (1994) Calibration and standardization of iatroscan (TLC-FID) using standards derived from crude oils. Org Geochem 22:835–862

  29. 29.

    Zhou X, Wangersky PJ (1987) Characterization of marine organic matter by thin-layer chromatography with flame ionization detection. Mar Chem 20:211–218

  30. 30.

    Karlsen DA, Larter SR (1991) Analysis of petroleum fractions by TLC-FID: application to petroleum reservoir description. Org Geochem 17:603–617

  31. 31.

    Volkman JK, Holdworth DG, Holdsworth DG, Niell GP (1992) Identification of natural, anthropogenic and petroleum hydrocarbons in aquatic sediments. Sci Total Environ 112:203–219

  32. 32.

    Wang S, Guo G, Yan Z, Lu G, Wang Q, Li F (2010) The development of a method for the qualitative and quantitative determination of petroleum components using thin-layer chromatography with flame ionization detector. J Chromatogr A 1217:368–374

  33. 33.

    Khan SA, Sarfraz S, Price D (2012) TLC-FID calibration of accurate weigh determination of SARA fractions in heavy crude oil. Pet Sci Technol 30:2401–2406

  34. 34.

    Cavanagh JE, Juhasz ALN, Franzmann PD, Mcmeekin TA (1995) Analysis of microbial hydrocarbon degradation using TLC FID. J Microbiol Methods 22:119–130

  35. 35.

    Jiang C, Larter SR, Noke KJ, Snowdon LR (2008) TLC-FID (iatroscan) analysis of heavy oil and tar sand samples. Org Geochem 39:1210–1214

  36. 36.

    Cebolla VL, Vela J, Membrado L, Ferrando AC (1998) Suitability of TLC-FID with regard to quantitative characterization of different fossil fuel products. 1. FID performances and response of pure compounds related to fossil fuel products. J Chromatogr Sci 36:479–486

  37. 37.

    Vela J, Membrado L, Cebolla VL, Ferrando AC (1998) Suitability of TLC-FID with regard to quantitative characterization of different fossil fuel products. II. Calibration methods concerning quantitative hydrocarbon-group type analysis. J Chromatogr Sci 36:487–494

  38. 38.

    Cagniant D, Nosyrev I, Cebolla V, Vella J, Membrano L, Gruber R (2001) Structural modifications of petroleum asphaltenes by reductive alkylation investigated by TLC-FID. Fuel 80:107–115

  39. 39.

    Radovic JR, Dominguez C, Laffont K, Diez S, Readman JW, Albaiges J, Bayona JM (2012) Compositional properties characterizing commonly transported oils and controlling their fate in the marine environment. J Environ Monit 14:3220–3229

  40. 40.

    Nowak P, Kosinska J, Glinka M, Kaminski M (2017) The thin-layer micro chromatography (µTLC) and TLC-FID technique as a new methodology in the study of lubricating oil. J AOAC Int 100:922–934

  41. 41.

    Ikebuchi J, Yuasa I, Kotoku S (1988) A rapid and sensitive method for the determination of paraquat in plasma and urine by thin-layer chromatography with flame ionization detector. J Anal Toxicol 12:80–83

  42. 42.

    Yamaoka K, Nakajima K, Moriyama H, Saito Y, Sato T (1986) Determination of sodium dodecyl sulphate in hydrophilic ointments by thin-layer chromatography with flame ionization detector. J Pharm Sci 75:606–607

  43. 43.

    Ono M, Shimamine M, Takahashi K (1978) Studies on psychotropic drugs. Determination of meprobamate by means of TLC-FID analyzer. Eisei Shikenjo Hokoku 96:67–70

  44. 44.

    Domingues C, Jover E, Garde F, Bayona JM, Erra P (2003) Characterization of supercritical fluid extracts from raw wool by TLC-FID and GC-MD. JAOCS 80:717–724

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The authors wish to thank Luc De Moor (former Expertise Centrum Manager Antwerp, Rotterdam & Amsterdam) for his contribution and the introduction of TLC–FID to the Bureau Laboratory, Antwerp, Belgium.

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Correspondence to Ann Van Schepdael.

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Anyakudo, F., Adams, E. & Van Schepdael, A. Thin-Layer Chromatography–Flame Ionization Detection. Chromatographia 83, 149–157 (2020). https://doi.org/10.1007/s10337-019-03849-z

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  • Iatroscan
  • Lipids
  • Petrochemicals
  • Chromarods