A Fast and Accurate Way to Determine Short Chain Fatty Acids in Human Serum by GC–MS and Their Distribution in Children with Digestive Diseases
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Short-Chain Fatty Acids (SCFAs) represent the largest group of metabolic nutrients produced by gut microbiota, and can be considered potential biomarkers for digestive tract diseases. A fast, cost-effective, and reproducible analytical method for the analysis of SCFAs is highly required. This work developed a one-step extraction of 14 SCFAs using a mixture of ethanol and n-hexane in human serum samples by GC–MS without derivatization. The recoveries of the 14 SCFAs ranged from 62.4% to 129.2% in human serum samples. The LODs and LOQs of the 14 SCFAs were 0.12–0.48 mg/L and 0.40–1.61 mg/L, respectively. The application of this method was carried out by comparing SCFAs in serum samples from children with health and digestive tract diseases, and the results demonstrated that SCFAs in the serum of children with digestive disorders were significantly higher than those of healthy children, implying that digestive tract diseases affect gut microbiota and their metabolisms.
KeywordsGas chromatography–mass spectrometry Short-chain fatty acids (SCFAs) Human serum Digestive tract diseases
This work is financially supported by the National Natural Science Foundation of China (No. 81803214). We express our thanks to Prof. Bing Shao and Prof. Jing Zhang from Beijing CDC providing the serum samples of healthy children.
This study was funded by the National Natural Science Foundation of China (Grant number: No. 81803214).
Compliance with Ethical Standards
Conflict of interest
All authors have been informed of the full content and approved the publication of this manuscript, and all authors declare that they have no conflict of interest.
All procedures performed in the study involving human participants were in accordance with the ethical standards of Beijing Children’s Hospital and Beijing CDC research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards (case number: 2016-10).
Informed consent was obtained from all individual participants included in the study.