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Rapid Detection of Short-Chain Fatty Acids in Biological Samples

  • Chaozheng ZhangEmail author
  • Longxing Fan
  • Hua ZhaoEmail author
Short Communication
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Abstract

An increasing number of researchers are focusing on short-chain fatty acids (SCFAs), which are closely related to various aspects of the human body. This study presented a pretreatment method for mouse feces that combined acidification and extraction. Then, a direct injection process into gas chromatography was developed. The limits of detection using this method are 0.5–0.8 mg L−1, and the corresponding sample contents are 0.015–0.024 mg g−1. The limits of quantification are 0.8–1.0 mg L−1, and the corresponding sample contents are 0.024–0.030 mg g−1. The linear relationship of the calibration curve is good and has a correlation coefficient of > 0.999. The recovery rate of the spiked standard is 80–102%. This method had been successfully applied to analysing and determining SCFAs in biological samples.

Graphic Abstract

Keywords

Short-chain fatty acids Acidification Extraction Gas chromatography Biological samples 

Notes

Acknowledgements

This work has been supported by the Foundation (No. ZXKF20180304) of Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control.

Funding

This work has been supported by the Foundation (No. ZXKF20180304) of Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control.

Compliance with Ethical Standards

Conflict of interest

All authors declare that there are no conflict of interests.

Ethical guidelines

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

10337_2019_3824_MOESM1_ESM.pdf (179 kb)
Supplementary material 1 (PDF 179 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Ministry of Education Industrial Fermentation Microbiology, Tianjin Key Laboratory of Industrial Microbiology, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of BiotechnologyTianjin University of Science and TechnologyTianjinPeople’s Republic of China

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