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System Maps for the Retention of Neutral Compounds on an Electrostatic-Shielded Reversed-Phase Column

  • Sanka N. Atapattu
  • Kevin R. D. Johnson
  • Colin F. PooleEmail author
Original
  • 15 Downloads

Abstract

The system constants of the solvation parameter model are used to prepare system maps for the retention of small neutral compounds on an octadecylsiloxane-bonded positive shield porous silica stationary phase (Luna Omega PS C18) for aqueous mobile phases containing 10–70% (v/v) methanol or acetonitrile. Electrostatic interactions (cation exchange) for weak bases were observed using acetonitrile–water but not methanol–water mobile phases at a similar level to a sterically shielded octadecylsiloxane-bonded silica column (Kinetex XB-C18). The system constants of the solvation parameter model and retention factor correlation plots for varied compounds indicated that the Luna Omega PS C18 stationary phase was (near) selectivity equivalent to the diisobutyloctadecylsiloxane-bonded superficially porous silica column Kinetex XB-C18. No specific interactions related to the embedded charged/chargeable functional group were observed compared with other high-purity type-B silica octadecylsiloxane-bonded stationary phases for typical reversed-phase separation conditions with unbuffered mobile phases.

Keywords

Reversed-phase liquid chromatography Retention Selectivity Solvation parameter model System maps Octadecylsiloxane-bonded silica stationary phase Positive shield Electrostatic shield Steric shield 

Notes

Compliance with Ethical Standards

Conflict of interest

The authors declare that external funding was not received for this study and the study protocols were not influenced by commercial considerations. The authors have no conflict of interest.

Supplementary material

10337_2019_3714_MOESM1_ESM.docx (127 kb)
Supplementary material 1 (DOCX 126 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.CanAm Bioresearch Inc.WinnipegCanada
  2. 2.Department of ChemistryWayne State UniversityDetroitUSA

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