, Volume 82, Issue 2, pp 605–613 | Cite as

A Systematic Method for Evaluating Quality of Jinqi Jiangtang Tablet: Simultaneous Determination of 21 Compounds by HPLC–MS

  • Zhangyao Xu
  • Yining Zhao
  • Zhipeng Qiu
  • Yuanyuan Li
  • Tianming Wang
  • Rong ShiEmail author
  • Yueming MaEmail author


Jinqi Jiangtang tablet (JQJTT), a traditional Chinese herbal medicine for treating diabetes, consists of Coptis chinensis, Astragalus membranaceus, and Lonicera japonica. In this study, we developed a method using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC–MS) to analyze 21 compounds in JQJTT, belonging to five different types. Separation was performed on a C18 column (150 × 4.6 mm, 5 µm), and the mobile phase for the gradient elution consisted of 2 mM ammonium acetate and 0.1% acetic acid as an aqueous phase and acetonitrile as an organic phase. The method showed good linearity (r > 0.9954). The relative standard deviation (RSD) values for the intra- and inter-day precision were less than 4.98% and 4.93%, respectively. The recovery at three concentration levels ranged from 90.15 to 109.90%, with RSD below 4.69%. Sample solutions were proven to be stable for more than 24 h at 4 °C (RSD < 4.87%). The novel method was successfully applied to systematic quality evaluation of JQJTT based on the quantification of the 21 compounds.


Jinqi Jiangtang tablets HPLC–MS Quality evaluation Traditional Chinese medicine 



The authors are grateful for the financial support from the National Natural Science Foundation of China (81303296), Xinglin scholar (2013), Graduate student innovation project of Shanghai University of Traditional Chinese Medicine (A1-182040225).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no competing interests.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of PharmacologyShanghai University of Traditional Chinese MedicineShanghaiChina

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