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Chromatographia

, Volume 80, Issue 1, pp 31–38 | Cite as

Resolving Co-Elution Problems of Components in Complex Mixtures by Multiple Heart-Cutting 2D-LC

  • Matthias PurschEmail author
  • Paul Lewer
  • Stephan Buckenmaier
Original

Abstract

Multiple heart-cutting (MHC) 2D-LC was applied to resolve challenging peak co-elutions in a pesticidal natural product-derived extract, which exhibited a complex impurity profile. While the 1D separation using sub-2 μm particle column technology provided good separation efficiency, additional selectivity was required to separate overlapping peaks in several chromatographic regions. MHC 2D-LC using C18 columns in both dimensions, but different pH in the mobile phase, provided full separation of the components of interest. This article describes how the MHC 2D-LC technology has been applied for optimized analysis of target components as well as being a rapid tool for peak purity evaluation. It also discusses strategic benefits of the technology regarding method development for separation of very complex samples.

Graphical abstract

Keywords

2D-LC Heart-cutting Natural products UHPLC Method development 

Notes

Acknowledgements

This work was in part funded by the Technology Development and Renewal (TRD) program in the Dow Analytical Technology Center.

Compliance with Ethical Standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. 1.
    Newman DJ, Cragg GM (2016) Natural products as sources of new drugs from 1981 to 2014. J Nat Prods 79:629–661CrossRefGoogle Scholar
  2. 2.
    Gerwick BC, Sparks TC (2014) Natural products for pest control: an analysis of their role, value and future. Pest Manag Sci 70:1169–1185CrossRefGoogle Scholar
  3. 3.
    Sauter H (2012) Strobilurins and other complex III inhibitors. In: Kraemer W (ed) Modern crop protection compounds, 2nd edn. Wiley-VCH Verlag GmbH & Co., KGaA, Weinheim, Germany, pp 584–627Google Scholar
  4. 4.
    Meinke PT, Smith MM and Shoop WL (2002) Nodulisporic acid: Its chemistry and biology, Curr Topics in Med Chem (Hilversum, Netherlands) 2:655–674Google Scholar
  5. 5.
    Dripps JE., Boucher RE, Chloridis A, Cleveland CB, Deamicis CV, Gomez LE, Paroonagian DL, Pavan, LA, Sparks TC, Watson GB (2011) The spinosyn insecticides, in RSC Green Chemistry Series 11:163–212Google Scholar
  6. 6.
    Dugo P, Cacciola F, Kumm T, Dugo G, Mondello L (2008) Comprehensive multidimensional liquid chromatography: theory and applications. J Chromatogr A 1184:353–368CrossRefGoogle Scholar
  7. 7.
    Sarrut M, Cretier G, Heinisch S (2014) Theoretical and practical interest in UHPLC technology for 2D-LC. Trends Anal Chem 63:104–112CrossRefGoogle Scholar
  8. 8.
    Stoll DR (2015) Recent advances in 2D-LC for bioanalysis. Bioanalysis 7:3125–3142CrossRefGoogle Scholar
  9. 9.
    Lee C, Zang J, Cuff J, McGachy N, Natishan TK, Welch CJ, Helmy R, Bernardoni F (2013) Application of heart-cutting 2D-LC for the determination of peak purity for a chiral pharmaceutical compound by HPLC. Chromatographia 76:5–11CrossRefGoogle Scholar
  10. 10.
    Groskreutz SR, Swenson MM, Secor LB, Stoll DR (2012) Selective comprehensive multi-dimensional separation for resolution enhancement in high performance liquid chromatography. Part I: Principles and instrumentation. J Chromatogr A 1228:31–40CrossRefGoogle Scholar
  11. 11.
    Groskreutz SR, Swenson MM, Secor LB, Stoll DR (2012) Selective comprehensive multidimensional separation for resolution enhancement in high performance liquid chromatography. Part II: Applications. J Chromatogr A 1228:41–50CrossRefGoogle Scholar
  12. 12.
    Larson ED, Groskreutz SR, Harmes DC, Gibbs-Hall IC, Trudo SP, Allen RC, Rutan SC, Stoll DR (2013) Development of selective comprehensive two-dimensional liquid chromatography with parallel first-dimension sampling and second-dimension separation-application to the quantitative analysis of furanocoumarins in apiaceous vegetables. Anal Bioanal Chem 405:4639–4653CrossRefGoogle Scholar
  13. 13.
    Stoll DR, Harmes DC, Danforth J, Wagner E, Guillarme D, Fekete S, Beck A (2015) Direct identification of rituximab main isoforms and subunit analysis by online selective comprehensive two-dimensional liquid chromatography-mass spectrometry. Anal Chem 87:8307–8315CrossRefGoogle Scholar
  14. 14.
    Venkatramani CJ, Girotti J, Wigman L, Chetwyn N (2014) Assessing stability-indicating methods for coelution by two-dimensional liquid chromatography with mass spectrometric detection. J Sep Sci 37:3214–3225CrossRefGoogle Scholar
  15. 15.
    Zhang K, Li Y, Tsang M, Chetwyn NP (2013) Analysis of pharmaceutical impurities using multi-heartcutting 2D LC coupled with UV-charged aerosol MS detection. J Sep Sci 36:2986–2992Google Scholar
  16. 16.
    Pursch M, Buckenmaier S (2015) Loop-based multiple heart-cutting two-dimensional liquid chromatography for target analysis in complex matrices. Anal. Chem. 87:5310–5317CrossRefGoogle Scholar
  17. 17.
    Ouyang Y, Zeng Y, Rong Y, Song Y, Shi L, Chen B, Yang X, Xu N, Linhardt RJ, Zhang Z (2015) Profiling analysis of low molecular weight heparins by multiple heart-cutting two dimensional chromatography with quadruple time-of-flight mass spectrometry. Anal Chem 87:8957–8963CrossRefGoogle Scholar
  18. 18.
    Qiao X, Wang Q, Song W, Qian Y, Xiao Y, An R, Guo D, Ye M (2016) A chemical profiling solution for Chinese medicine formulas using comprehensive and loop-based multiple heart-cutting two-dimensional liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. J Chromatogr A 1438:198–204CrossRefGoogle Scholar
  19. 19.
    Yao C, Yang W, Wu W, Da J, Hou J, Zhang J, Zhang Y, Jin Y, Yang M, Jiang B, Liu X, Guo D (2015) Simultaneous quantitation of five Panax notoginseng saponins by multi heart-cutting two-dimensional liquid chromatography: Method development and application to the quality control of eight notoginseng containing Chinese patent medicines. J Chromatogr A 1402:71–81CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Matthias Pursch
    • 1
    Email author
  • Paul Lewer
    • 2
  • Stephan Buckenmaier
    • 3
  1. 1.Dow Deutschland Anlagen GmbHAnalytical SciencesStadeGermany
  2. 2.Dow AgroSciences LLCBioengineering and Bioprocessing R&DIndianapolisUSA
  3. 3.Agilent TechnologiesResearch and DevelopmentWaldbronnGermany

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