, Volume 80, Issue 1, pp 31–38 | Cite as

Resolving Co-Elution Problems of Components in Complex Mixtures by Multiple Heart-Cutting 2D-LC

  • Matthias PurschEmail author
  • Paul Lewer
  • Stephan Buckenmaier


Multiple heart-cutting (MHC) 2D-LC was applied to resolve challenging peak co-elutions in a pesticidal natural product-derived extract, which exhibited a complex impurity profile. While the 1D separation using sub-2 μm particle column technology provided good separation efficiency, additional selectivity was required to separate overlapping peaks in several chromatographic regions. MHC 2D-LC using C18 columns in both dimensions, but different pH in the mobile phase, provided full separation of the components of interest. This article describes how the MHC 2D-LC technology has been applied for optimized analysis of target components as well as being a rapid tool for peak purity evaluation. It also discusses strategic benefits of the technology regarding method development for separation of very complex samples.

Graphical abstract


2D-LC Heart-cutting Natural products UHPLC Method development 



This work was in part funded by the Technology Development and Renewal (TRD) program in the Dow Analytical Technology Center.

Compliance with Ethical Standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Matthias Pursch
    • 1
    Email author
  • Paul Lewer
    • 2
  • Stephan Buckenmaier
    • 3
  1. 1.Dow Deutschland Anlagen GmbHAnalytical SciencesStadeGermany
  2. 2.Dow AgroSciences LLCBioengineering and Bioprocessing R&DIndianapolisUSA
  3. 3.Agilent TechnologiesResearch and DevelopmentWaldbronnGermany

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