Chromatographia

, Volume 79, Issue 3–4, pp 125–136 | Cite as

Screening and Research of Anti-Cancer Matrine Components Based on Hollow Fiber Cell Fishing with High-Performance Liquid Chromatography

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Abstract

Hollow fiber cell fishing with high-performance liquid chromatography (HFCF-HPLC) based on human breast cancer MCF-7 cells, human renal tubular ACHN cells, human ovarian cancer SKOV-3 cells, and hepatoma HepG-2 cells, and their cell membranes, cell organelles, or receptors was developed and employed to research the active matrine alkaloids group, such as oxymatrine, matrine, sophoridine, and oxysophocarpine, from radix sophorae flavescentis (RSF) and its injection in vitro. Hollow fiber liquid-phase microextraction with HPLC (HF-LPME-HPLC) was conducted to extract and detect the active components in vivo. Some of the structures of the active components screened were identified in vitro. The binding sites and targets of the potential active matrine compounds were preliminarily researched. The effective anti-cancer components were determined, and the efficacy material basis of both medicines on matrine alkaloids was elucidated. Before the application of HFCF-HPLC, the survival rate of cells seeded on the fiber, death rate after screening components, non-specific binding between active centers on the fiber and target components, positive and negative controls, and repeatability of the tests were investigated. Results demonstrated that HFCF-HPLC, coupled with HF-LPME-HPLC, is a simple, quick, and reliable method to screen active components, determine truly effective components, and elucidate preliminarily the characteristics of multiple components and multiple targets for traditional Chinese medicine.

Keywords

Hollow fiber liquid-phase microextraction Hollow fiber cell fishing Cancer cell Receptor Matrine alkaloids 
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Notes

Acknowledgments

This study was supported by the National Nature Science Foundation of China (No. 81302734) and the Nature Science Foundation of Shanxi Province, China (No. 2011011035-2, 2012011007-4).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.School of Pharmacy, College of PharmacyShanxi Medical UniversityTaiyuanChina

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