Advertisement

Chromatographia

, Volume 78, Issue 3–4, pp 291–295 | Cite as

Fast GC Analysis of Fatty Acid Methyl Esters Using a Highly Polar Ionic Liquid Column and its Application for the Determination of Trans Fatty Acid Contents in Edible Oils

  • Shinsuke InagakiEmail author
  • Masahiko Numata
Short Communication

Abstract

A simple and rapid methyl esterification technique for edible oils and a high-throughput procedure for the separation and quantification of fatty acid methyl esters including cis/trans isomers by fast GC using a highly polar ionic liquid column have been established. The developed method was successfully applied to the determination of trans fatty acid contents in commercially available edible oils, such as margarine, fat spread, shortening, olive oil, rapeseed oil, salad oil, and sesame oil. Combining the developed methyl esterification and fast GC techniques reduced the time required for the analysis of one edible oil specimen to less than 30 min; thus, the analysis time was shortened substantially to less than one-third that required in the existing official methods such as those of the American Oil Chemists’ Society and Japanese Oil Chemists’ Society. The contents determined by the developed method agreed with those determined by the existing official methods, thus confirming the reliability of the fast GC method.

Keywords

Fast GC Ionic liquid Edible oil Trans fatty acid Methyl esterification 

Notes

Acknowledgments

This work was supported by Grants-in-Aid for Young Scientists (B24780138) from the Japan Society for the Promotion of Science (JSPS).

References

  1. 1.
    Mossoba MM, Kramer JKG (2009) Official methods for the determination of trans fat, 2nd edn. AOCS Press, UrbanaGoogle Scholar
  2. 2.
    Mozaffarian D, Katan MB, Ascherio A, Stampfer MJ, Willett WC (2006) N Engl J Med 354:1601–1613CrossRefGoogle Scholar
  3. 3.
    Ascherio A, Hennekens CH, Buring JE, Master C, Stampfer MJ, Willett WC (1994) Circulation 89:94–101CrossRefGoogle Scholar
  4. 4.
    American Oil Chemists’ Society (2009) Official Methods and Recommended Practices of the AOCS. AOCS, Official Method Ce 1h-05Google Scholar
  5. 5.
    AOAC International (2001) Official Methods of Analysis. AOAC International, Method 996.06Google Scholar
  6. 6.
    Shirasawa S, Shiota M, Arakawa H, Shigemitsu Y, Yokomizo K, Shionoya N, Okamoto T, Miyazaki Y, Takahashi S, Himata K (2007) J Oleo Sci 56:405–415CrossRefGoogle Scholar
  7. 7.
    Japan Oil Chemists’ Society (2013) Standard Methods for the Analysis of Fats, Oils and Related Materials. 2.4.4.3-2013Google Scholar
  8. 8.
    Wolff RL, Bayard CC (1995) J Am Oil Chem Soc 72:1197–1201CrossRefGoogle Scholar
  9. 9.
    Ratnayake WMN, Plouffe LJ, Pasquier E, Gaston C (2002) J AOAC Int 85:1112–1118Google Scholar
  10. 10.
    Rodríguez-Alcalá LM, Calvo MV, Fontecha J (2013) J Chromatogr Sci 51:70–81CrossRefGoogle Scholar
  11. 11.
    Cramers CA, Janssen H-G, van Deursen MM, Leclercq PA (1999) J Chromatogr A 856:315–329CrossRefGoogle Scholar
  12. 12.
    Korytár P, Janssen H-G, Matisová E, Brinkman UAT (2002) Trends Anal Chem 21:558–572CrossRefGoogle Scholar
  13. 13.
    Delmonte P, Kia A-RF, Kramer JKG, Mossoba MM, Sidisky L, Rader JI (2011) J Chromatogr A 1218:545–554CrossRefGoogle Scholar
  14. 14.
    American Oil Chemists’ Society (2009) Official Methods and Recommended Practices of the AOCS. Official Method Ce 2–66Google Scholar
  15. 15.
    Japan Oil Chemists’ Society (2013) Standard Methods for the Analysis of Fats, Oils and Related Materials. 2.4.1.2-2013Google Scholar
  16. 16.
    MacGee J, Allen KG (1974) J Chromatogr 100:35–42CrossRefGoogle Scholar
  17. 17.
    Kossa WC, MacGee J, Ramachandran S, Webber AJ (1979) J Chromatogr Sci 17:177–187CrossRefGoogle Scholar
  18. 18.
    Challinor JM (1996) J Anal Appl Pyrolysis 37:185–197CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Organic Standards Section, Organic Analytical Chemistry DivisionNational Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan

Personalised recommendations