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Chromatographia

, Volume 77, Issue 13–14, pp 893–899 | Cite as

Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase

  • Tengfei Chen
  • Jiangyong Gu
  • Hao Wang
  • Gu Yuan
  • Lirong Chen
  • Xiaojie Xu
  • Wei Xiao
Original

Abstract

In this work, a core–shell molecularly imprinted polymer (MIP) was synthesized through sol–gel coating procedure by using silica beads, emodin, 3-aminopropyltriethoxysilane, tetraethoxysilane and tetrahydrofuran as supporting matrix, template, functional monomer, cross-linker and solvent, respectively. The selective recognition property for emodin and its analogues (physcion and aloe emodin) of the resultant MIP was evaluated in a chromatographic column for high-performance liquid chromatography (HPLC). The retention time and imprinted factor of MIP column for emodin, physcion and aloe emodin were 5.11, 0.63, 0.69 min and 4.69, 0.75, 1.38, respectively. It showed that the MIP beads had a good binding ability for emodin. Finally, one-step separation of emodin from alcohol extract of Rheum palmatum L. at semi-preparative scale was achieved and 380 mg of emodin was collected in 5 days. The product was characterized by mass spectrometry and HPLC and its purity was 95 %.

Keywords

Liquid chromatography Molecularly imprinted polymer Semi-preparative separation Emodin Rheum palmatum L. 

Notes

Acknowledgments

This work was financially supported by National Key Special Project of Science and Technology for Innovation Drugs (Grant Nos. 2008ZX09401-006, 2009ZX09502-015 and 2012ZX09501001-004) and the country support National science and technology project (2008BAI51BOO).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Tengfei Chen
    • 1
    • 2
    • 3
  • Jiangyong Gu
    • 2
  • Hao Wang
    • 2
  • Gu Yuan
    • 2
  • Lirong Chen
    • 2
  • Xiaojie Xu
    • 2
  • Wei Xiao
    • 1
    • 3
  1. 1.School of Chinese Materia MedicaBeijing University of Chinese MedicineBeijingChina
  2. 2.Beijing National Laboratory for Molecular Sciences, State Key Lab of Rare Earth Material Chemistry and ApplicationsCollege of Chemistry and Molecular Engineering, Peking UniversityBeijingChina
  3. 3.State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical ProcessJiangsu Kanion Pharmaceutical Co., LtdLianyungangChina

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