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Chromatographia

, Volume 77, Issue 11–12, pp 821–828 | Cite as

Cyclodextrin-Modified Gold Nanoparticle Capillary Electrochromatography with Online Sample Stacking for Simultaneous and Sensitive Determination of Aminobenzoic Acid Isomers

  • Wenhua Sun
  • Yalei Dong
  • Hong Cui
  • Hong Zhao
  • Yujian He
  • Yongsheng Ding
  • Xiangjun LiEmail author
  • Zhuobin Yuan
Original

Abstract

A newly-developed method of complete separation and sensitive determination of o-, m-, and p-aminobenzoic acid isomers was achieved by combining open-tubular columns for capillary electrochromatography (OT-CEC) and online sample stacking. In this study, spherical gold nanoparticles were modified by a covalent attachment of mono-6-thio-β-cyclodextrin, and OT-CEC was formed by immobilizing cyclodextrin-modified gold nanoparticles (CD-AuNP) on prederivatized 3-mercaptopropyl-trimethoxysilane fused-silica capillaries. Based on the theory of moving chemical reaction boundary, effects of several important factors such as the pH and concentration of running buffer and the conditions of stacking analytes were optimized. The optimized separations were carried out in 58 mmol/L HAc buffer at pH 3.0 using a capillary coated with CD-AuNP, while the optimized concentration was carried out in 50 mmol/L disodium hydrogen phosphate (pH 9.5). The linear ranges for m-, p-, and o-aminobenzoic acid were from 5.0 × 10−4–0.1, 5.0 × 10−4–0.1 and 1.0 × 10−4–0.1 mmol/L, respectively. And the detection limits (S/N = 3) were as low as 8.22 × 10−5, 8.21 × 10−5, and 3.76 × 10−5 mmol/L for m-, p-, and o-aminobenzoic acid, respectively. The run-to-run, day-to-day, and column-to-column reproducibilities of migration time were satisfactory with relative standard deviation values of less than 4.5 % in all cases. This method was successfully used in determining procaine hydrochloride injection sample with recoveries in the range of 96.1–106.6 % and relative standard deviations less than 5.0 %.

Keywords

Capillary electrochromatography Gold nanoparticles Online concentration 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 21145006) and the Foundation for the Returned Overseas Chinese Scholars.

Conflict of interest

The authors have declared no conflict of interest.

Supplementary material

10337_2014_2686_MOESM1_ESM.pdf (1008 kb)
Supplementary material 1 (PDF 1,007 kb)

References

  1. 1.
    Boughtflower RJ, Underwood T, Paterson CJ (1995) Chromatographia 40:329–335CrossRefGoogle Scholar
  2. 2.
    Gübitz G, Schmid MG (2008) J Chromatogr A 1204:140–156CrossRefGoogle Scholar
  3. 3.
    Guihen E, Glennon JD (2004) J Chromatogr A 1044:67–81CrossRefGoogle Scholar
  4. 4.
    Wang YC, Zeng ZR, Xie CH, Guan N, Fu EQ, Cheng JK (2001) Chromatographia 54:475–479CrossRefGoogle Scholar
  5. 5.
    Nilsson C, Birnbaum S, Nilsson S (2007) J Chromatogr A 1168:212–224CrossRefGoogle Scholar
  6. 6.
    Yu CJ, Su CL, Tseng WL (2006) Anal Chem 78:8004–8010CrossRefGoogle Scholar
  7. 7.
    Li M, Liu X, Jiang F, Guo L, Yang L (2011) J Chromatogr A 1218:3725–3729CrossRefGoogle Scholar
  8. 8.
    Hongjun E, Yang Y, Su P, Zhang W (2009) J Anal Chem 64:393–397CrossRefGoogle Scholar
  9. 9.
    Dai R, Tang L, Li H, Deng Y, Fu R, Parveen Z (2007) J Appl Polym Sci 106:2041–2046CrossRefGoogle Scholar
  10. 10.
    Wang Y, Zeng Z, Guan N, Cheng J (2001) Electrophoresis 22:2167–2172CrossRefGoogle Scholar
  11. 11.
    Frost NW, Jing M, Bowser MT (2010) Anal Chem 82:4682–4698CrossRefGoogle Scholar
  12. 12.
    Zhu Z, Zhou X, Yan N, Zhou L, Chen X (2010) J Chromatogr A 217:1856–1861CrossRefGoogle Scholar
  13. 13.
    Cao CX, He YZ, Li M, Qian YT, Yang L, Qu QS, Zhou SL, Chen WK (2002) J Chromatogr A 952:39–46CrossRefGoogle Scholar
  14. 14.
    Cao CX, He YZ, Li M, Qian YT, Gao MF, Ge LH, Zhou SL, Yang L, Qu QS (2002) Anal Chem 74:4167–4174CrossRefGoogle Scholar
  15. 15.
    Burgi DS (1993) Anal Chem 65:3726–3729CrossRefGoogle Scholar
  16. 16.
    Chien RL, Burgi DS (1992) Anal Chem 64:489A–496ACrossRefGoogle Scholar
  17. 17.
    Quirino JP, Iwai Y, Otsuka K, Terabe S (2000) Electrophoresis 21:2899–2903CrossRefGoogle Scholar
  18. 18.
    Quirino JP, Terabe S (1999) Anal Chem 71:1638–1644CrossRefGoogle Scholar
  19. 19.
    Quirino JP, Terabe S (2000) Anal Chem 72:1023–1030CrossRefGoogle Scholar
  20. 20.
    Kim B, Chung DS (2002) Electrophoresis 23:49–55CrossRefGoogle Scholar
  21. 21.
    Weiss DJ, Saunders K, Lunte CE (2001) Electrophoresis 22:59–65CrossRefGoogle Scholar
  22. 22.
    He JF, Yang WY, Yao FJ, Zhao H, Li XJ, Yuan ZB (2011) J Chromatogr A 1218:3816–3821CrossRefGoogle Scholar
  23. 23.
    Frost NW, Jing M, Bowser MT (2010) Anal Chem 82:4682-4698Google Scholar
  24. 24.
    Zhu W, Zhang W, Fan LY, Shao J, Li S, Chen JL, Cao CX (2009) Talanta 78:1194–1200CrossRefGoogle Scholar
  25. 25.
    Jiang S, ter Horst JH, Jansens PJ (2007) Cryst Growth Des 8:37–43CrossRefGoogle Scholar
  26. 26.
    Svärd M, Nordström FL, Jasnobulka T, Rasmuson ÅC (2009) Cryst Growth Des 10:195–204CrossRefGoogle Scholar
  27. 27.
    He Y, Wu C, Kong W (2005) J Phys Chem 109:748–753CrossRefGoogle Scholar
  28. 28.
    Terekhova IV (2009) Mendeleev Commun 19:110–112CrossRefGoogle Scholar
  29. 29.
    Tatsuya Kitade KK (1998) Yutaka Wada. Anal Chim Acta 367:33–39CrossRefGoogle Scholar
  30. 30.
    Panadero S, Gómez-Hens A, Pérez-Bendito D (1998) Talanta 45:829–834CrossRefGoogle Scholar
  31. 31.
    Chen Y, Han FM, Yuan ZB (1996) Chin J Anal Lab 15:55–57Google Scholar
  32. 32.
    Turkevich J (1985) Gold Bull 18:86–91CrossRefGoogle Scholar
  33. 33.
    Jiang Y, Zhao H, Zhu NN, Lin YQ, Yu P, Mao LQ (2008) Angew. Chem Int Ed 47:8601–8604CrossRefGoogle Scholar
  34. 34.
    Liang X, Wei H, Cui Z, Deng J, Zhang Z, You X, Zhang XE (2011) Analyst 136:179–183CrossRefGoogle Scholar
  35. 35.
    Řezanka P, Navrátilová K, Žvátora P, Sýkora D, Matějka P, Mikšík I, Kašička V, Král V (2011) J Nanopart Res 13:5947–5957CrossRefGoogle Scholar
  36. 36.
    Shuang SM, Yang Y, Pan JH (2002) Anal Chim Acta 458:305–310CrossRefGoogle Scholar
  37. 37.
    Bartle KD, Myers P (2001) J Chromatogr A 916:3–23CrossRefGoogle Scholar
  38. 38.
    Yang Y, Shuang SM, Chao JB (2004) Acta Chim Sin 62:176–182Google Scholar
  39. 39.
    Cao CX, Zhang W, Qin WH, Li S, Zhu W, Liu W (2005) Anal Chem 77:955–963CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Wenhua Sun
    • 1
  • Yalei Dong
    • 1
  • Hong Cui
    • 1
  • Hong Zhao
    • 1
  • Yujian He
    • 1
  • Yongsheng Ding
    • 2
  • Xiangjun Li
    • 1
    Email author
  • Zhuobin Yuan
    • 1
  1. 1.College of Chemistry and Chemical EngineeringUniversity of Chinese Academy of SciencesBeijingChina
  2. 2.College of Life SciencesUniversity of Chinese Academy of SciencesBeijingChina

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