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Chromatographia

, Volume 76, Issue 23–24, pp 1721–1727 | Cite as

HPLC Method for the Simultaneous Determination of the UV-Filters Butyl Methoxy Dibenzoylmethane and Octocrylene in the Presence of Their Photodegradants

  • Jutta Kockler
  • Cherie A. Motti
  • Sherryl Robertson
  • Michael Oelgemöller
  • Beverley D. GlassEmail author
Original

Abstract

Butyl methoxy dibenzoylmethane (BMDM) and octocrylene (OC), common UV-filters in sunscreen products are often used in combination. Together they provide broad spectrum photoprotection from exposure to both UVA- and UVB-light. These UV-filters may, however, undergo photodegradation and generate photodegradants, resulting in a potential loss of photoprotection. It is thus a concern that the photostability testing as described by the ICH Guideline Q1B is not a requirement for sunscreen products in Australia, Europe or the USA. UV-filter photodegradants have in addition been shown to be toxic, highlighting the importance of their separation from the parent UV-filters. An HPLC method was developed and validated to quantitatively determine a combination of these UV-filters in the presence of their photodegradants. Reverse-phase chromatography was employed, using a C18 column and an isocratic mobile phase consisting of methanol/water/acetic acid (89/10/1 v/v). Validation according to the ICH guidelines for linearity, accuracy, precision, sensitivity, specificity and robustness was confirmed. The developed and validated method was then successfully applied to the determination of BMDM and OC in an aqueous cream base, typically used in sunscreens, after photostability testing, according to the ICH Guideline Q1B. In addition, the diketo-enol ratio of BMDM in methanol-d 4 was determined by NMR and the two major photodegradants were identified by FTMS and LC–MS.

Keywords

HPLC UV-filter and photodegradants Butyl methoxy dibenzoylmethane Octocrylene Method validation Aqueous cream 

Notes

Acknowledgments

JK would like to thank the School of Pharmacy and Molecular Science at James Cook University (JCU) for a scholarship and the Graduate Research School at JCU for a Graduate Research Scheme Grant in 2012.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Jutta Kockler
    • 1
  • Cherie A. Motti
    • 2
  • Sherryl Robertson
    • 1
  • Michael Oelgemöller
    • 1
  • Beverley D. Glass
    • 1
    Email author
  1. 1.School of Pharmacy and Molecular SciencesJames Cook UniversityTownsvilleAustralia
  2. 2.Australian Institute of Marine ScienceTownsvilleAustralia

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