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Chromatographia

, Volume 76, Issue 11–12, pp 629–633 | Cite as

An Efficient Separation Method of Polysaccharides: Preparation of an Antitumor Polysaccharide APS-2 from Auricularia polytricha by Radial Flow Chromatography

  • Jingbo Wang
  • Xiuping Li
  • Guanglei Song
Original

Abstract

Auricularia polytricha is an edible mushroom, quite popular in China. Modern pharmacology research indicate that A. polytricha polysaccharides possess antitumor and immunomodulatory activities. In this paper, an antitumor Auricularia polytricha polysaccharide (APS)-2 was purified by radial flow chromatography (RFC) in a column of 500 mL bed-volume (40 cm i.d × 15 cm) packed with DEAE52-cellulose anion ion-exchange resin. The optimal separation conditions were: sample flow-velocity 15–20 mL min−1, sample volume 160–200 mL, and elution with distilled water and 0.2 M NaCl at a velocity of 35–45 mL min−1. The yield and content of APS-2 obtained under these conditions were 182 mg g−1 total polysaccharides and 98.35 %, respectively. The study provides guidelines for the separation and purification of polysaccharides using a radial flow chromatography column.

Keywords

Radial flow chromatography Antitumor Polysaccharide Auricularia polytricha 

Notes

Acknowledgments

This work was supported by a grant (31101272) from National Natural Science Foundation of China and a grant (2012C22098) from the Science Technology Department of Zhejiang Province.

References

  1. 1.
    Sun YX, Liu JC (2008) Int J Biol Macromol 43:279CrossRefGoogle Scholar
  2. 2.
    Murata Y, Shimamura T, Tagami T, Takatsuki F, Hamuro J (2002) Int Immunopharmacol 2:673CrossRefGoogle Scholar
  3. 3.
    Markova N, Kussovski V, Drandarska I, Nikolaeva S, Georgieva N, Radoucheva T (2003) Int Immunopharmacol 3:1557CrossRefGoogle Scholar
  4. 4.
    Luo X, Yu MY, Jiang N, Xu XY, Zeng J, Zheng LY (2009) Mycosystema 28:435Google Scholar
  5. 5.
    Mau JL, Chao GR, Wu KT (2001) J Agric Food Chem 49:5461CrossRefGoogle Scholar
  6. 6.
    Yang BK, Ha JY, Jeong SC, Jeon YJ, Ra KS, Das S (2002) Biotechnol Lett 24:1319CrossRefGoogle Scholar
  7. 7.
    Qin CG, Huang KX, Xu HB (2002) Carbohyd Polym 49:367CrossRefGoogle Scholar
  8. 8.
    Tamura H, Tanaka S, Oda T, Uemura Y, Aketagawa J, Hashimoto Y (1996) Carbohyd Res 295:103Google Scholar
  9. 9.
    Dai YJ, Wang JW, Jia SR, Yue SJ, Jia MY, Xu P (2009) Biotechnol Bioprocess Eng 14:377CrossRefGoogle Scholar
  10. 10.
    Jiang HY, Sun PL, He JZ, Shao P (2012) Food Bioprod Process 90:1CrossRefGoogle Scholar
  11. 11.
    Demirci A, Leu F, Bailey FJ (2012) Am J Biochem Biotechnol 8:255CrossRefGoogle Scholar
  12. 12.
    McCartney JE (1991) Biotechniques 11:648Google Scholar
  13. 13.
    Sun T, Chen G, Liu Y, Bu F, Wen M (2000) Biomed Chromatogr 14:478CrossRefGoogle Scholar
  14. 14.
    Song G, Du Q (2010) J Chromatogr A 1217:5930CrossRefGoogle Scholar
  15. 15.
    Kim GY, Park HS, Nam BH, Lee SJ, Lee JD (2003) Bioresour Technol 89:81CrossRefGoogle Scholar
  16. 16.
    Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Anal Chem 28:350CrossRefGoogle Scholar
  17. 17.
    Sun T, Chen G, Liu Y, Bu F, Wen M (2000) J Chromatogr B 742:109CrossRefGoogle Scholar
  18. 18.
    Scopes RK (1996) Biotechnol Appl Biochem 23(Pt3):197–204Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Institute of Food and Biological EngineeringZhejiang Gongshang UniversityHangzhouChina

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