Chromatographia

, Volume 76, Issue 9–10, pp 483–489 | Cite as

HPLC Method for Chiral Separation and Quantification of Antidepressant Citalopram and Its Precursor Citadiol

  • Radim Geryk
  • Jiří Vozka
  • Květa Kalíková
  • Eva Tesařová
Original
First Online:
Received:
Revised:
Accepted:

Abstract

HPLC method enabling chiral separation and determination of citalopram (CIT), a widely used antidepressant, and its synthetic precursor citadiol in one analysis was developed and validated. Moreover, supercritical fluid chromatography was also tested and was proved to be less effective for this separation purpose. The optimized HPLC system was composed of Chiralcel OD-H column and n-hexane/propane-2-ol/triethylamine 96/4/0.1 (v/v/v) as mobile phase, column temperature 25 °C, flow rate 1.0 mL min−1, UV detection at 250 nm. The effects of amount of propane-2-ol, triethylamine addition, and temperature on enantioselectivity and resolution of the enantiomers were evaluated. The method was found to be suitable for determination of the enantiomeric purity of CIT in bulk drugs. Enantiomers of CIT were determined in two commercially available pharmaceuticals.

Keywords

HPLC SFC Chiral separations Citalopram Citadiol Chiralcel OD-H column 
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Notes

Acknowledgments

The Grant Agency of the Charles University in Prague, Project No. 356411, KONTAKT AM 2010 Project LH11018, Project UNCE 204018/304018/2012 and the long-term project MSM0021620857 of the Ministry of Education, Youth and Sports of the Czech Republic are gratefully acknowledged for the financial support. The authors want to express their gratitude to Prof. G. E. Scriba for his generous gift of the enantiomers tested. Prof. Ch. Roussel and Dr. N. Vanthuyne from the Chirosciences team of Aix-Marseille University are acknowledged for their kind help during the SFC experiments. The bilateral cooperation between Charles University in Prague and Aix-Marseille University was established in the frame of LPP-ERASMUS PROGRAMME.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Radim Geryk
    • 1
  • Jiří Vozka
    • 1
  • Květa Kalíková
    • 1
  • Eva Tesařová
    • 1
  1. 1.Department of Physical and Macromolecular Chemistry, Faculty of ScienceCharles University in PraguePrague 2Czech Republic

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