, Volume 76, Issue 19–20, pp 1239–1247 | Cite as

Planar Chromatographic Systems in Pattern Recognition and Fingerprint Analysis

  • Dušanka Milojković-OpsenicaEmail author
  • Petar Ristivojević
  • Filip Andrić
  • Jelena Trifković


An overview of opportunities of contemporary planar chromatography in pattern recognition and fingerprint analysis is presented. The most used chemometric methods are highlighted and their main advantages and drawbacks are underlined. In addition a cross section of the application of planar chromatographic fingerprinting in food, pharmaceutical, environmental, and forensic analysis is given.


Review Planar chromatography Fingerprint analysis Pattern recognition methods 



This work has been supported by the Ministry of Education and Science of the Republic of Serbia, Grant No. 172017.


  1. 1.
    Ni Y, Zhang L, Churchill J, Kokot S (2007) Application of high performance liquid chromatography for the profiling of complex chemical mixtures with the aid of chemometrics. Talanta 72:1533–1539CrossRefGoogle Scholar
  2. 2.
    Zarzycki PK, Zarzycka MB, Clifton VL, Adamski J, Głód BK (2011) Low-parachor solvents extraction and thermostated micro-thin-layer chromatography separation for fast screening and classification of spirulina from pharmaceutical formulations and food samples. J Chromatogr A 1218:5694–5704CrossRefGoogle Scholar
  3. 3.
    Sherma J (2003) Basic TLC techniques, materials, and apparatus. In: Sherma J, Fried B (eds) Handbook of thin-layer chromatography, 3rd edn. Marcel Dekker, New YorkCrossRefGoogle Scholar
  4. 4.
    Andrić FLj, Trifković JÐ, Tešić ŽLj, Milojković-Opsenica DM (2008) An approximate linear solvation energy relationships model based on snyder’s selectivity parameters. Chromatographic behavior of some1-aralkyl-4-arylpiperazines. Chromatographia 68(5/6):453–458CrossRefGoogle Scholar
  5. 5.
    Atrrog AAB, Natić M, Tosti T, Milojković-Opsenica D, Đorđević I, Tešević V, Jadranin M, Milosavljević S, Lazić M, Radulović S, Tešić Ž (2009) Lipophilicity of some guaianolides isolated from two endemic subspecies of Amphoricarpos neumayeri (Asteraceae) from Montenegro. Biomed Chromatogr 23:250–256CrossRefGoogle Scholar
  6. 6.
    Radoičić A, Majstorović H, Sabo T, Tešić Ž, Milojković-Opsenica D (2009) Hydrophilic-interaction planar chromatography of some water-soluble Co(III) complexes on different adsorbents. J Planar Chromatogr 22(4):249–253CrossRefGoogle Scholar
  7. 7.
    Andrić FLj, Trifković JĐ, Radoičić AD, Šegan SB, Tešić ŽLj, Milojković-Opsenica DM (2010) Determination of the soil-water partition coefficient (log KOC) of some mono- and poly-substituted phenols by reversed-phase thin-layer chromatography. Chemosphere 81:299–305CrossRefGoogle Scholar
  8. 8.
    Trifković JĐ, Andrić FLj, Ristivojević P, Andrić D, Tešić ŽLj, Milojković-Opsenica DM (2010) Structure–retention relationship study of arylpiperazines by linear multivariate modeling. J Sep Sci 33:2619–2628CrossRefGoogle Scholar
  9. 9.
    Tosti T, Natić M, Smoliński A, Milić D, Milojković-Opsenica D, Tešić Ž (2011) Study of retention of 31 polyoxygenated steroids by normal- and reversed-phase thin-layer chromatography. Acta Chromatogr 23(3):429–445CrossRefGoogle Scholar
  10. 10.
    Šegan S, Trifković J, Verbić T, Opsenica D, Zlatović M, Burnett J, Šolaja B, Milojković-Opsenica D (2013) Correlation between structure, retention, property, and activity of biologically relevant 1,7-bis(aminoalkyl)diazachrysene derivatives. J Pharm Biomed Anal 72:231–239CrossRefGoogle Scholar
  11. 11.
    Krasikov VD (2003) Contemporary planar chromatography. J Anal Chem 58:706–719CrossRefGoogle Scholar
  12. 12.
    Siouffi A-M (2005) From paper to planar: 60 years of thin layer chromatography. Sep Purif Rev 34:155–180CrossRefGoogle Scholar
  13. 13.
    Stahl E (1956) Thin-layer chromatography. Pharmazie 11:633–637Google Scholar
  14. 14.
    Kirchner JG (1967) Thin layer chromatography. In: Perry ES, Weissberger A (eds) Techniques of organic chemistry, 1st edn. Wiley, New YorkGoogle Scholar
  15. 15.
    Shewiyo DH, Kaale E, Risha PG, Dejaegher B, Smeyers-Verbeke J, Vander Heyden Y (2012) HPTLC methods to assay active ingredients in pharmaceutical formulations: a review of the method development and validation steps. J Pharm Biomed Anal 66:11–23CrossRefGoogle Scholar
  16. 16.
    Poole CF (2003) Thin-layer chromatography: challenges and opportunities. J Chromatogr A 1000:963–984CrossRefGoogle Scholar
  17. 17.
    Vailaya A (2005) Fundamentals of reversed phase chromatography: thermodynamic and exothermodynamic treatment. J Liquid Chromatogr Relat Technol 28:965–1054CrossRefGoogle Scholar
  18. 18.
    Morlock G, Schwack W (2008) Planar chromatography—back to the future? LC GC Eur 21:366–371Google Scholar
  19. 19.
    Gupta S, Shanker K, Srivastava SK (2012) HPTLC method for the simultaneous determination of four indole alkaloids in Rauwolfiatetraphylla: a study of organic/green solvent and continuous/pulse sonication. J Pharm Biomed Anal 66:33–39CrossRefGoogle Scholar
  20. 20.
    Poole SK, Poole CF (2011) High performance stationary phases for planar chromatography. J Chromatogr A 1218:2648–2660CrossRefGoogle Scholar
  21. 21.
    Frolova AM, Konovalova OY, Loginova LP, Bulgakova AV, Boichenko AP (2011) Thin-layer chromatographic plates with monolithic layer of silica: production, physical–chemical characteristics, separation capabilities. J Sep Sci 34:2352–2361Google Scholar
  22. 22.
    Bezuidenhout LW, Brett MJ (2008) Ultrathin layer chromatography on nanostructured thin films. J Chromatogr A 1183:179–185CrossRefGoogle Scholar
  23. 23.
    Clark JE, Olesik SV (2009) Technique for ultrathin layer chromatography using an electrospun, nanofibrous stationary phase. Anal Chem 81:4121–4129CrossRefGoogle Scholar
  24. 24.
    Jim SR, Oko AJ, Taschuk MT, Brett MJ (2011) Morphological modification of nanostructured ultrathin-layer chromatography stationary phases. J Chromatogr A 1218:7203–7210CrossRefGoogle Scholar
  25. 25.
    Kowalska T, Aboul-Enein HY, Vander Heyden Y, Vovk I, Waksmundzka-Hajnos M (2012) Pharmaceutical and herbal fingerprinting by means of chromatographic techniques. Chromatogr Res Int. doi: 10.1155/2012/809541 Google Scholar
  26. 26.
    Yan YZ, Xie PS, Lam WK, Chui E, Yu QX (2010) Study on triterpenoic acids distribution in Ganoderma mushrooms by automatic multiple development high performance thin layer chromatographic fingerprint analysis. J AOAC Int 93:1384–1389Google Scholar
  27. 27.
    Lianga YZ, Xieb P, Chanc K (2004) Quality control of herbal medicines. J Chromatogr B 812:53–70Google Scholar
  28. 28.
    Reich E, Schibli A (2008) Validation of high-performance thin-layer chromatographic methods for the identification of botanicals in a cGMP environment. J AOAC Int 91:13–20Google Scholar
  29. 29.
    Widmer V, Reich E, De Batt A (2008) Validated HPTLC method for identification of Hoodia gordonii. J Planar Chromatogr Mod TLC 21:21–26CrossRefGoogle Scholar
  30. 30.
    Li Z, Merfort I (2010) High-performance thin layer chromatography for quality control of multicomponent herbal drugs: example of Cangzhu Xianglian San. J AOAC Int 93:1390–1398Google Scholar
  31. 31.
    Cui S, Fu B, Lee FSC, Wang X (2005) Application of microemulsion thin layer chromatography for the fingerprinting of licorice (Glycyrrhiza spp.). J Chromatogr B 828:33–40CrossRefGoogle Scholar
  32. 32.
    Zarzycki PK, Slaczka MM, Zarzycka MB, Włodarczyk E, Baran MJ (2011) Application of micro-thin-layer chromatography as a simple fractionation tool for fast screening of raw extracts derived from complex biological, pharmaceutical and environmental samples. Anal Chim Acta 688:168–174CrossRefGoogle Scholar
  33. 33.
    Yamunadevi M, Wesely EG, Johnson M (2011) Chromatographic fingerprint analysis of steroids in Aerva lanata L. by HPTLC technique. Asian Pac J Trop Biomed 1:428–433CrossRefGoogle Scholar
  34. 34.
    Yamunadevi M, Wesely EG, Johnson M (2012) Chromatographic fingerprint studies on saponins of Aerva lanata (L.) Juss. Ex Schultes by using HPTLC. Int J Curr Pharm Res 4:52–57Google Scholar
  35. 35.
    Yamunadevi M, Wesely EG, Johnson M (2012) Chromatographic fingerprint analysis on flavonoids constituents of the medicinally important plant Aerva lanata L. by HPTLC technique. Asian Pac J Trop Biomed 1:S8–S12Google Scholar
  36. 36.
    Hariprasad P, Ramakrishnan N (2012) Chromatographic finger print analysis of Rumex vesicarius L. by HPTLC technique. Asian Pac J Trop Biomed, S57–S63Google Scholar
  37. 37.
    Hariprasad P, Ramakrishnan N (2011) Chromatographic finger print analysis of Naringi crenulata by HPTLC technique. Asian Pac J Trop Biomed S195–S198Google Scholar
  38. 38.
    Parul S, Anubhuti P, Rahul S, Chandrakant K, Sanju N (2011) Identification and standardization of some herbal drugs, their extracts and marketed formulations by HPTLC fingerprinting. J Pharm Biomed Sci 6:1–3Google Scholar
  39. 39.
    Sunil K, Sayeed A, Paras S (2011) Pharmacognostic evaluation and HPTLC fingerprinting of Nicotiana tabacum leaf collected from different geographical regions of India. Pharm Lett 3:291–300Google Scholar
  40. 40.
    Kumar VK, Kumar PS, Rajan M, Kumar AV, Boppana R, Reddy PS, Alzeber HFH (2011) Qualitative phytochemical analysis of Bauhinia tomentosa Linn flower by HPTLC. J Pharm Res 4:2868–2880Google Scholar
  41. 41.
    Prakash KC, Garg SK, Yadav P, Dey A, Dey S (2011) Studies on chromatographic finger print analysis and antibacterial activity of Adhatoda vasica leaves extracts. Pharmacologyonline 3:1322–1329Google Scholar
  42. 42.
    Kesavanarayanan KS, Kalaivani P, Sathiya S, Ranju V, Sunil AG, Saravanababu C, Kavimani S, Prathiba D (2011) Standardization of an herbal formulation (DIA-2) containing Allium sativum and Lagerstroemia speciosa extracts using HPTLC–UV densitometry. J Pharm Res 4:3910–3914Google Scholar
  43. 43.
    Sajewicz M, Staszek D, Natic M, Waksmundzka-Hajnos M, Kowalska T (2011) TLC–MS versus TLC–LC–MS fingerprints of herbal extracts. Part III. Application of the reversed-phase liquid chromatography systems with C18 stationary phase. J Chromatogr Sci 49:560–567CrossRefGoogle Scholar
  44. 44.
    Sajewicz M, Wojtal L, Natić M, Staszek D, Waksmundzka-Hajnos M, Kowalska T (2011) TLC–MS versus TLC–LC–MS fingerprints of herbal extracts. Part I. Essential oils. J Liquid Chromatogr Relat Technol 34:848–863CrossRefGoogle Scholar
  45. 45.
    Sajewicz M, Staszek D, Natić M, Wojtal L, Waksmundzka-Hajnos M, Kowalska T (2011) TLC–MS versus TLC–LC–MS fingerprints of herbal extracts. Part II. Phenolic acids and flavonoids. J Liquid Chromatogr Relat Technol 34:864–887CrossRefGoogle Scholar
  46. 46.
    Reich E, Blatter A (2003) Herbal drugs, herbal drug preparations, and herbal medicinal products. In: Sherma J, Fried B (eds) Handbook of thin-layer chromatography, 3rd edn. Marcel Dekker, New YorkGoogle Scholar
  47. 47.
    Waksmundzka-Hajnos M, Sherma J, Kowalska T (2008) Thin layer chromatography in phytochemistry. CRC Press, Taylor & Francis GroupGoogle Scholar
  48. 48.
    Reich E, Schibli A (2007) High-performance thin-layer chromatography for the analysis of medicinal plants. Thieme Medical Publishers, Inc., New YorkGoogle Scholar
  49. 49.
    Nicoletti M (2011) HPTLC fingerprint: a modern approach for the analytical determination of Botanicals. Braz J Pharmacogn 21:818–823Google Scholar
  50. 50.
    Babu SK, Kumar KV, Subbaraju GV (2005) Estimation of trans-resveratrol in herbal extracts and dosage forms by high-performance thin-layer chromatography. Chem Pharm Bull 53:691–693CrossRefGoogle Scholar
  51. 51.
    Gunther M, Schmidt PC (2005) Comparison between HPLC and HPTLC–densitometry for the determination of harpagoside from Harpagophytum procumbens CO2-extracts. J Pharm Biomed Anal 37:817–821CrossRefGoogle Scholar
  52. 52.
    Cieśla Ł, Staszek D, Hajnos M, Kowalska T, Waksmundzka-Hajnos M (2011) Development of chromatographic and free radical scavenging activity fingerprints by thin-layer chromatography for selected Salvia species. Phytochem Anal 22:59–65CrossRefGoogle Scholar
  53. 53.
    Cieśla Ł (2012) Biological fingerprinting of herbal samples by means of liquid chromatography. Chromatogr Res Int. doi: 10.1155/2012/532418 Google Scholar
  54. 54.
    Salmon CNA, Bailey-Shaw YA, Hibbert S, Green C, Smith AM, Williams LAD (2012) Characterisation of cultivars of Jamaican ginger (Zingiber officinale Roscoe) by HPTLC and HPLC. Food Chem 131:1517–1522CrossRefGoogle Scholar
  55. 55.
    Hirsh AVI (2007) Digitally enhanced thin-layer chromatography: an inexpensive, new technique for qualitative and quantitative analysis. J Chem Educ 84:842–847CrossRefGoogle Scholar
  56. 56.
    Komsta Ł (2012) Chemometrics in fingerprinting by means of thin layer chromatography. Chromatogr Res Int. doi: 10.1155/2012/893246 Google Scholar
  57. 57.
    Djozan D, Baheri T, Karimian G, Shahidi M (2008) Forensic discrimination of blue ballpoint pen inks based on thin layer chromatography and image analysis. Forensic Sci Int 179:199–205CrossRefGoogle Scholar
  58. 58.
    Olech M, Komsta Ł, Nowak R, Ciesla Ł, Waksmundzka-Hajnos M (2012) Investigation of antiradical activity of plant material by thin-layer chromatography with image processing. Food Chem 132:549–553CrossRefGoogle Scholar
  59. 59.
    Daszykowski M, Stanimirova I, Bodzon-Kulakowska A, Silberring J, Lubec G, Walczak B (2007) Start-to-end processing of two-dimensional gel electrophoretic images. J Chromatogr A 1158:306–317CrossRefGoogle Scholar
  60. 60.
    Savitzky A, Golay MJE (1964) Smoothing and differentiation of data by simplified least squares procedures. Anal Chem 36:1627–1639CrossRefGoogle Scholar
  61. 61.
    Komsta Ł (2009) A comparative study on several algorithms for denoising of thin layer densitograms. Anal Chim Acta 641:52–58CrossRefGoogle Scholar
  62. 62.
    Komsta Ł, Ciesla Ł, Bogucka-Kocka A, Józefczyk A, Kryszen J, Waksmundzka-Hajnos M (2011) The start-to-end chemometric image processing of 2D thin-layer videoscans. J Chromatogr A 1218:2820–2825CrossRefGoogle Scholar
  63. 63.
    Ogegbo OL, Eyob S, Parmar S, Wanga Z-T, Bligha ASW (2012) Metabolomics of four TCM herbal products: application of HPTLC analysis. Anal Methods 4:2522–2527CrossRefGoogle Scholar
  64. 64.
    Tomasi G, van den Bergand F, Andersson C (2004) Correlation optimized warping and dynamic time warping as preprocessing methods for chromatographic data. J Chemom 18:231–241CrossRefGoogle Scholar
  65. 65.
    Walczak B, Wub W (2005) Fuzzy warping of chromatograms. Chemom Intell Lab Syst 77:173–180CrossRefGoogle Scholar
  66. 66.
    Lavine BK (2006) Pattern recognition. Crit Rev Anal Chem 36:153–161CrossRefGoogle Scholar
  67. 67.
    Brereton R (2003) Chemometrics data analysis for the laboratory and chemical plant. Wiley, ChichesterGoogle Scholar
  68. 68.
    Varmuza K, Filzmoser P (2008) Introduction to multivariate statistical analysis in chemometrics. Taylor & Francis group LLC, Boca RatonGoogle Scholar
  69. 69.
    Otto M (1999) Chemometrics, statistics and computer application in analytical chemistry. Wiley-VCH, WeinheimGoogle Scholar
  70. 70.
    Sârbu C, Mot AC (2011) Ecosystem discrimination and fingerprinting of Romanian propolis by hierarchical fuzzy clustering and image analysis of TLC patterns. Talanta 85:1112–1117CrossRefGoogle Scholar
  71. 71.
    Neumann C, Margot P (2009) New perspectives in the use of ink evidence in forensic science Part II. Development and testing of mathematical algorithms for the automatic comparison of ink samples analysed by HPTLC. Forensic Sci Int 185:38–50CrossRefGoogle Scholar
  72. 72.
    Tian R, Xie P, Liu H (2009) Evaluation of traditional Chinese herbal medicine: Chaihu (Bupleuri Radix) by both high-performance liquid chromatographic and high-performance thin-layer chromatographic fingerprint and chemometric analysis. J Chromatogr A 1216:2150–2155CrossRefGoogle Scholar
  73. 73.
    Bro R (1997) PARAFAC tutorial and applications. Chemom Intell Lab Sys 38:149–171CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Dušanka Milojković-Opsenica
    • 1
    Email author
  • Petar Ristivojević
    • 2
  • Filip Andrić
    • 1
  • Jelena Trifković
    • 1
  1. 1.Faculty of ChemistryUniversity of BelgradeBelgradeSerbia
  2. 2.Innovation Center, Faculty of Chemistry LtdBelgradeSerbia

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