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Chromatographia

, Volume 76, Issue 7–8, pp 345–353 | Cite as

Study of Interaction between Metallothionein and CdTe Quantum Dots

  • Sylvie Skalickova
  • Ondrej Zitka
  • Lukas Nejdl
  • Sona Krizkova
  • Jiri Sochor
  • Libor Janu
  • Marketa Ryvolova
  • David Hynek
  • Jarmila Zidkova
  • Vaclav Zidek
  • Vojtech Adam
  • Rene KizekEmail author
Original

Abstract

Quantum dots (QDs) belong to a new class of fluorescent agent for biochemical, medicinal or other purposes. However, QDs based on cadmium or other metals can be risky for an organism. As one of the mechanism how to detoxify cadmium-based QDs expression of metallothioneins (MT) can be considered. Due to high affinity of metallothionein to cadmium(II) ions, we attempted to develop an approach for studying of possible interaction with QDs. We prepared QDs with CdTe core and studied the interaction with MT, which we isolated from livers of Cd-administered rabbits. To study the interaction, we used the mixture of both components MT (3.6 μM): CdTe QDs (0, 0.34, 0.68, 1.02, 1.36, 1.7, 2.04 and 2.47 μM). The mixtures were studied by spectrophotometry within the range from 200 to 750 nm with detected maxima at 260 and 505 nm. Same mixtures were also analysed by differential pulse voltammetry Brdicka reaction, which supported data from spectrophotometry. Subsequently, we used fast protein liquid chromatography for purification of protein–quantum dot conjugates. We obtained the different chromatograms for (1) Apo MT, (2) CdTe QDs and (3) MT–QD complex. We also collected the fractions and subsequently analysed them on the content of Cd and MT, which confirmed the formation of CdTe QDs–MT complex.

Keywords

Fast protein liquid chromatography Brdicka reaction Differential pulse voltammetry Fluorimetry Spectrophotometry Separation of quantum dots ApoMT 

Notes

Acknowledgments

Financial support from the following projects IGA TP 6/2012, NANIMEL GACR 102/08/1546, CEITEC CZ.1.05/1.1.00/02.0068 and IAA600110902 is highly acknowledged. The authors thank Pavel Kopel for the technical support.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Sylvie Skalickova
    • 1
  • Ondrej Zitka
    • 1
    • 2
  • Lukas Nejdl
    • 1
  • Sona Krizkova
    • 1
    • 2
  • Jiri Sochor
    • 1
    • 2
  • Libor Janu
    • 1
  • Marketa Ryvolova
    • 1
    • 2
  • David Hynek
    • 1
    • 2
  • Jarmila Zidkova
    • 3
  • Vaclav Zidek
    • 3
  • Vojtech Adam
    • 1
    • 2
  • Rene Kizek
    • 1
    • 2
    Email author
  1. 1.Department of Chemistry and Biochemistry, Faculty of AgronomyMendel University in BrnoBrnoCzech Republic
  2. 2.Central European Institute of Technology, Brno University of TechnologyBrnoCzech Republic
  3. 3.Department of Biochemistry and MicrobiologyInstitute of Chemical TechnologyPragueCzech Republic

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