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Chromatographia

, Volume 76, Issue 19–20, pp 1333–1337 | Cite as

Miniaturized HPTLC of Vitamin B12 Compounds in Foods

  • Tomohiro Bito
  • Yukinori Yabuta
  • Fumio WatanabeEmail author
Short Communication

Abstract

High-performance thin-layer chromatography (HPTLC) is a separation technique commonly used to identify and quantify compounds in chemical mixtures. Preliminary experiments indicated that Lichrospher silica gel 60 F254s HPTLC sheets were the most suitable for analyzing vitamin B12 compounds. This study revealed the advantages of miniaturized Lichrospher HPTLC for analyzing authentic vitamin B12 compounds as short migration distances (5 cm) and short development times (<45 min) in combination with high separation efficiency and sensitivity (>25 pmol at 254 nm). The practicability of using miniaturized HPTLC was demonstrated by the separation and identification of vitamin B12 compounds purified from foods using an immunoaffinity column.

Keywords

High-performance thin-layer chromatography Miniaturized planar chromatography Lichrospher silica gel 60 F254s Food analysis Vitamin B12 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Tomohiro Bito
    • 1
  • Yukinori Yabuta
    • 1
  • Fumio Watanabe
    • 1
    Email author
  1. 1.Division of Applied Bioresources Chemistry, The United Graduate School of Agricultural SciencesTottori UniversityTottoriJapan

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