Chromatographia

, Volume 75, Issue 15–16, pp 857–866 | Cite as

LC–MS–MS Method for the Simultaneous Determination of Donepezil Enantiomers in Plasma

  • Adriana Iordachescu
  • Luigi Silvestro
  • Ariana Tudoroniu
  • Simona Rizea Savu
  • Valentin Ciuca
Original

Abstract

This paper presents a new analytical method with adequate sensitivity, precision, accuracy, and specificity to quantitatively determine (−)-donepezil and (+)-donepezil in plasma, at concentrations that can be expected in real samples of pharmacokinetic studies. This method combines a HPLC separation on cellulose tris (3,5-dimethylphenyl carbamate) coated on 5-μm silica-gel column known as CHIRALCEL OD–RH eluted with mobile phase consisting of acetonitrile and ammonium bicarbonate and MS–MS detection. Under the HPLC conditions used (−)-donepezil and (+)-donepezil, as well as the enantiomers of the internal standard d7-donepezil, eluted at 5 and 6.25 min, respectively. The curve fittings were optimal for the entire calibration range (0.05–25.0 ng mL−1) with correlation coefficients (r) = 0.999 for (−)-donepezil and (r) = 0.999 for (+)-donepezil (linear regression model with 1/x2 weighing). The assay method showed a good specificity for donepezil enantiomers, and it could be successfully applied to pharmacokinetic studies.

Keywords

LC–MS–MS HPLC Donepezil Enantiomers Pharmacokinetic Chiral stationary phase 

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Adriana Iordachescu
    • 1
    • 2
  • Luigi Silvestro
    • 3
  • Ariana Tudoroniu
    • 1
  • Simona Rizea Savu
    • 3
  • Valentin Ciuca
    • 1
  1. 1.Pharma Serv Int’l SRLBucharestRomania
  2. 2.Department of Analytical Chemistry, Faculty of ChemistryUniversity of BucharestBucharestRomania
  3. 3.3S-Pharmacological Consultation and Research GmbHHarpstedtGermany

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