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Chromatographia

, Volume 75, Issue 15–16, pp 847–855 | Cite as

Separation of para- and meta-Imazamethabenz-methyl Enantiomers by Direct Chiral HPLC Using a Protein Chiral Selector

  • Javier Zurita-Pérez
  • María Jesús Santos-DelgadoEmail author
  • Esther Crespo-Corral
  • Luis María Polo-Díez
  • Alfonso Aguilar-Gallardo
Original

Abstract

A high performance liquid chromatography direct chiral method for the determination of p- and m-imazamethabenz-methyl (IMBM) enantiomers is described. The four isomers were separated using a protein chiral AGP™ column. Elution order was established by circular dichroism. Factorial design was employed for mobile phase optimization using a small number of experiments. The best experimental conditions were ACN/NH4Ac-HAc 60 mM (3/97) at a flow rate of 0.9 mL min−1 in isocratic mode, detection at 247 nm wavelength and 24 °C temperature. Resolution up to 1.3 was obtained for the separation of the 4 IMBM enantiomers. The proposed method is useful to determine the p/m IMBM ratios and their respective enantiomers in samples containing the aforementioned herbicide. The p/m IMBM ratio was higher in the standard than in the sample. LOD values were between 0.15 and 0.43 mg L−1 and recoveries were higher than 93 %.

Keywords

Column liquid chromatography IMBM enantiomers Protein chiral selector Formulation analysis p/m Imazamethabenz-methyl ratio 

Notes

Acknowledgments

The authors thank Dirección General de Universidades e Investigación, Ministerio de Educación y Ciencia de Madrid for supporting Project CTQ2007-66971/BQU.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Javier Zurita-Pérez
    • 1
  • María Jesús Santos-Delgado
    • 1
    Email author
  • Esther Crespo-Corral
    • 1
  • Luis María Polo-Díez
    • 1
  • Alfonso Aguilar-Gallardo
    • 2
  1. 1.Department of Analytical Chemistry, Faculty of ChemistryComplutense University of MadridMadridSpain
  2. 2.Department of Analytical Chemistry, Faculty of SciencesUniversity of MalagaMalagaSpain

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