Separation of para- and meta-Imazamethabenz-methyl Enantiomers by Direct Chiral HPLC Using a Protein Chiral Selector
- 234 Downloads
A high performance liquid chromatography direct chiral method for the determination of p- and m-imazamethabenz-methyl (IMBM) enantiomers is described. The four isomers were separated using a protein chiral AGP™ column. Elution order was established by circular dichroism. Factorial design was employed for mobile phase optimization using a small number of experiments. The best experimental conditions were ACN/NH4Ac-HAc 60 mM (3/97) at a flow rate of 0.9 mL min−1 in isocratic mode, detection at 247 nm wavelength and 24 °C temperature. Resolution up to 1.3 was obtained for the separation of the 4 IMBM enantiomers. The proposed method is useful to determine the p/m IMBM ratios and their respective enantiomers in samples containing the aforementioned herbicide. The p/m IMBM ratio was higher in the standard than in the sample. LOD values were between 0.15 and 0.43 mg L−1 and recoveries were higher than 93 %.
KeywordsColumn liquid chromatography IMBM enantiomers Protein chiral selector Formulation analysis p/m Imazamethabenz-methyl ratio
The authors thank Dirección General de Universidades e Investigación, Ministerio de Educación y Ciencia de Madrid for supporting Project CTQ2007-66971/BQU.
- 23.Carlson R (2005) Design and optimization in organic synthesis. Elsevier, The NetherlandGoogle Scholar
- 25.Jin L, Gao W, Li L, Ye J, Lin C, Liu W (2011) Enantioseparation and enantioselective analysis of chiral herbicides. In: Herbicides, Theory and Applications. InTech. http://www.intechopen.com/source/pdfs/13144