Chromatographia

, Volume 75, Issue 17–18, pp 1009–1016 | Cite as

Analysis of Reducing Carbohydrates and Fructosyl Saccharides in Maple Syrup and Maple Sugar by CE

Original

Abstract

Reducing carbohydrates in maple syrup and maple sugar were separated by capillary electrophoresis using derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) and the characteristics of these samples were studied. Reducing carbohydrate standards including nine monosaccharides and five disaccharides as PMP derivatives could be easily resolved by using 200 mM borate buffer (pH 10.5) as a background electrolyte. Glucose was the most abundant reducing sugar in both maple samples, and mannose was abundant relative to the other sugars. The other monosaccharides (xylose, arabinose, ribose, galactose and N-acetylglucosamine) were also detected. When maple syrup and maple sugar were treated with invertase, which removed fructose residues from the reducing ends of fructosyl saccharides, melibiose was detected, suggesting that raffinose exists in both samples. The differences of carbohydrate contents between maple syrup and maple sugar were also discussed.

Keywords

Capillary electrophoresis Carbohydrates Maple syrup Maple sugar 1-Phenyl-3-methyl-5-pyrazolone 

Abbreviations

BGE

Background electrolyte

PMP

1-Phenyl-3-methyl-5-pyrazolone

Lac

Lactose

Mal

Maltose

Gen

Gentiobiose

Mel

Melibiose

Cel

Cellobiose

Gal

Galactose

Man

Mannose

Glc

Glucose

Rib

Ribose

Lyx

Lyxose

Xyl

Xylose

Ara

Arabinose

GalNAc

N-Acetylgalactosamine

GlcNAc

N-Acetylglucosamine

LC

Liquid chromatography

GC

Gas chromatography

ESI

Electrospray ionization

MS

Mass spectrometry

UV

Ultraviolet

r

Correlation coefficient

RSD

Relative standard deviation

S/N

Signal to noise ratio

LOD

Limit of detection

CE

Capillary electrophoresis

CZE

Capillary zone electrophoresis

MEKC

Micellar electrokinetic chromatography

IEKC

Ion exchange electrokinetic chromatography

CEC

Capillary electrochromatography

ACE

Affinity capillary electrophoresis

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Faculty of PharmacyKinki UniversityHigashi-OsakaJapan
  2. 2.Toyama Institute of HealthToyamaJapan

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