, 74:549 | Cite as

Simultaneous Estimation of Ceftazidime and Ceftizoxime in Pharmaceutical Formulations by HPLC Method

  • Senem Sanli
  • Nurullah Sanli
  • Mehmet Gumustas
  • Sibel A. Ozkan
  • Nurgul Karadas
  • Hassan Y. Aboul-EneinEmail author


The aim of the present study was to develop a fast, sensitive and reliable method for rapid screening of cephalosporin injectable dosage forms namely ceftazidime and ceftizoxime to the detection of counterfeit and substandard drugs that might be illegally commercialized. Ceftazidime, ceftizoxime and cefixime (IS) were separated in a X-Terra RP-18 column (250 × 4.60 mm ID × 5 μ) and DAD detector set at 290 and 260 nm. The mobile phase consisted of a mixture of methanol:water 20:80 (v/v) at a flow rate of 1.0 mL min−1. Additionally, in order to find the optimum pH value of separation the pK a values of studied compounds were determined by using two different methodologies. Aqueous pK a values of studied compounds have been determined by UV-spectrophotometry and liquid chromatography were used for the determination and direct characterization of the dissociation constants by using the dependence of the capacity factor on the pH of the mobile phase in 20% (v/v) methanol–water binary mixture in which separation was performed. The pH of the mobile phase was adjusted with 25 mM H3PO4 to 3.2. The method was shown to be linear, sensible, accurate, and reproducible over the range of analysis and it can be used to pharmaceutical formulations containing a single active ingredient within a short analysis time.


Column liquid chromatography UV-spectrophotometry Ceftazidime Ceftizoxime pKa values 



We gratefully acknowledge to Dr. Jose Luis Beltran from University of Barcelona for kindly providing the NLREG 4.0 and STAR programs.


  1. 1.
    BC Centre for Disease Control Knowledgebase (2008) Annual summary of antibiotics utilization. Accessed 1 Feb 2011
  2. 2.
    Thompson RL (1987) Mayo Clin Proc 62:821–834Google Scholar
  3. 3.
    Sanders CC, Sanders WE (1986) J Infect Dis 154:792–800CrossRefGoogle Scholar
  4. 4.
    Bethesda MD (1989) American hospital formulary service: drug information 89. American Society of Hospital Pharmacists, pp 119–123Google Scholar
  5. 5.
    Balant L, Dayer P, Auckenthaler R (1985) Clin Pharmacokinet 10:101–143CrossRefGoogle Scholar
  6. 6.
    Gluck SJ, Cleveland JA Jr (1994) J Chromatogr A 680:43–48CrossRefGoogle Scholar
  7. 7.
    Mrestani Y, Reinhard N, Angela M, Michael W (1998) J Chromatogr A 803:273–278CrossRefGoogle Scholar
  8. 8.
    Poole CF, Poole SK (1991) Chromatography today. Elsevier, AmsterdamGoogle Scholar
  9. 9.
    Schoenmakers PJ, Tijsen R (1993) J Chromatogr A 656:577–590CrossRefGoogle Scholar
  10. 10.
    Albert A, Sergeant EP (1984) Ionization constants of acids and bases. Chapman and Hall, LondonGoogle Scholar
  11. 11.
    Demiralay EC, Alsancak G, Ozkan SA (2009) J Sep Sci 32:2928–2936CrossRefGoogle Scholar
  12. 12.
    Gumustas M, Sanli S, Sanli N, Ozkan SA (2010) Talanta 82:1528–1537CrossRefGoogle Scholar
  13. 13.
    Mitchell RC, Salter CJ, Tam KY (1999) J Pharm Biomed Anal 20:289–295CrossRefGoogle Scholar
  14. 14.
    Hardcastle JE, Jano I (1998) J Chromatogr B 717:39–56CrossRefGoogle Scholar
  15. 15.
    Şanlı S, Şanlı N, Alsancak G (2009) J Braz Chem Soc 20:939–946Google Scholar
  16. 16.
    Şanlı S, Altun Y, Şanlı N, Alsancak G, Beltran J (2009) J Chem Eng Data 54:3014–3021CrossRefGoogle Scholar
  17. 17.
    Nahata MC, Morosco RS (1992) J Liq Chromatogr 15:1507–1511CrossRefGoogle Scholar
  18. 18.
    Pehourcq F, Jarry C (1998) J Chromatogr A 812:159–178CrossRefGoogle Scholar
  19. 19.
    Barnes AR (1995) J Liq Chromatogr 18:3117–3128CrossRefGoogle Scholar
  20. 20.
    Abdel-Hamid ME (1998) Il Farmaco 53:132–138CrossRefGoogle Scholar
  21. 21.
    Moore CM, Sato K, Katsumata Y (1991) J Chromatogr A 539:215–220CrossRefGoogle Scholar
  22. 22.
    Williams D (2002) pK a values for some drugs and miscellaneous organic acids and bases. In: Williams DA, Lemke TL (ed) Foye’s principles of medicinal chemistry, edn 5, Lippincott Williams and Wilkins, Philadelphia, pp 1071Google Scholar
  23. 23.
    El-Shaboury Salwa RA, Saleh Gamal A, Mohamed Fardous A, Rageh Azza H (2007) J Pharm Biomed Anal 45:1–19CrossRefGoogle Scholar
  24. 24.
    Evagelou V, Tsantili-Kakoulidou A, Koupparis M (2003) J Pharm Biomed Anal 31:1119–1128CrossRefGoogle Scholar
  25. 25.
    Aleksic M, Savic V, Popovic G, Buric N, Kapetanovic V (2005) J Pharm Biomed Anal 39:752–756CrossRefGoogle Scholar
  26. 26.
    Rondinini S, Mussini PR, Mussini T (1987) Pure Appl Chem 59:1549–1560CrossRefGoogle Scholar
  27. 27.
    Mussini PR, Mussini T, Rondinini S (1997) Pure Appl Chem 69:1007–1014CrossRefGoogle Scholar
  28. 28.
    Marcus Y (1989) J Chem Soc Faraday Trans 85:381–388CrossRefGoogle Scholar
  29. 29.
    Gran G (1952) Analyst 7:661–671CrossRefGoogle Scholar
  30. 30.
    Beltran JL, Codony R, Prat MD (1993) Anal Chim Acta 276:441–454CrossRefGoogle Scholar
  31. 31.
    Sherrod PH (2010) NLREG, v.4.0.
  32. 32.
    Pistos CM, Tsantili-Kakoulidou A, Koupparis M (2003) J Chrom Rel Tech 26:937–952CrossRefGoogle Scholar
  33. 33.
    Kasai M, Hatano S, Kitagawa M, Yoshimi A, Nishimura K, Mori N, Sakai A, Sugihara T (1999) Chem Pharm Bull 47:1081–1088Google Scholar
  34. 34.
    Riley CM, Rosanske TW (1996) Development and validation of analytical methods. Elsevier, New YorkGoogle Scholar
  35. 35.
    Swartz ME, Krull IS (1997) Analytical development and validation. Marcel Dekker, New YorkGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Senem Sanli
    • 1
  • Nurullah Sanli
    • 1
  • Mehmet Gumustas
    • 1
    • 2
  • Sibel A. Ozkan
    • 2
  • Nurgul Karadas
    • 1
  • Hassan Y. Aboul-Enein
    • 3
    Email author
  1. 1.Department of Chemistry, Faculty of Science and ArtsHitit UniversityÇorumTurkey
  2. 2.Department of Analytical Chemistry, Faculty of PharmacyAnkara UniversityAnkaraTurkey
  3. 3.Pharmaceutical and Medicinal Chemistry DepartmentNational Research CentreCairoEgypt

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