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Chromatographia

, Volume 73, Issue 9–10, pp 871–877 | Cite as

Development of a Parallel-Tandem Column Interface in a Two-Dimensional Liquid Chromatography System

  • Duxin Li
  • Lingyi Zhang
  • Zhicong Wang
  • Azeem Intisar
  • Weibing ZhangEmail author
Original

Abstract

An ion exchange-reversed-phase two-dimensional liquid chromatography (2D-LC) system was developed based on a newly designed interface. The interface was constructed by alternately connecting tandem columns to one of the parallel separation columns to increase the separation efficiency. Separation on the tandem column does not affect the switching frequency; the valve switching could be maintained at high frequency. The constructed interface could be used in both ambient and elevated temperature modes. Under high-temperature mode, only the tandem column was heated, which simplified the operation, decreased the backpressure, improved the solvent compatibility, and speeded up the separation. Tryptic digestion of four proteins was used to evaluate the performance of the developed system. The peak capacity of the 2D-LC system reached 650 under ambient conditions and 890 under high temperature conditions. When compared with the traditional parallel column interface constructed in this paper, the new interface produced a 60% higher peak capacity.

Keywords

Two-dimensional liquid chromatography system Ion exchange-reversed-phase chromatography Parallel-tandem column interface Peptides 

Notes

Acknowledgments

We are thankful to the NSFC (no. 20675083), 973 Program (no. 2007CB914100), and Key Projects in the National Science and Technology Pillar Program during the 11th Five-Year Plan Period (no. 2006BAF07B03) for financial support.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Duxin Li
    • 1
  • Lingyi Zhang
    • 1
  • Zhicong Wang
    • 1
  • Azeem Intisar
    • 1
  • Weibing Zhang
    • 1
    Email author
  1. 1.Shanghai Key Laboratory of Functional Materials ChemistryEast China University of Science and TechnologyShanghaiPeople’s Republic of China

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