Chromatographia

, Volume 73, Supplement 1, pp 131–135 | Cite as

HPLC Method for Monitoring of Degradation Products in Picoplatin Stability Study

  • Jitka Housková
  • Michaela Hrdá
  • Kamila Syslová
  • Petr Novotný
  • Petr Kačer
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Abstract

An analytical method combining high-performance liquid chromatography (HPLC) with UV detection was developed for an easy and rapid assay determination of the anticancer drug picoplatin (=cis-[Pt(NH3)(2-methylpyridine)Cl2]) and its degradation products. An ion exchanger was used as the stationary phase with an aqueous solution of NaH2PO4 with pH adjusted to 3.0 with H3PO4 as the mobile phase. The calibration curve was linear within the concentration range of 0.10–0.50 g L−1 (R2 ≥ 0.998). The limit of detection was 0.05 g L−1 and limit of quantification was 0.09 g L−1. The developed method was characterized with a high precision (≤6.0%, determined as RSD), an acceptable accuracy (the values of recovery were from intervals 98–103%). The developed method was used for assessing the stability of picoplatin during a stability study.

Keywords

Column liquid chromatography Stress stability study Anticancer drug Picoplatin 

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Jitka Housková
    • 1
  • Michaela Hrdá
    • 1
  • Kamila Syslová
    • 1
  • Petr Novotný
    • 2
  • Petr Kačer
    • 1
  1. 1.Department of Organic Technology, Faculty of Chemical TechnologyInstitute of Chemical TechnologyPragueCzech Republic
  2. 2.Essence LinePragueCzech Republic

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