, 73:857 | Cite as

Effect of Functionalized Stationary Phases on the Mechanism of Retention of Fungicides in RP-LC Elution

  • Sylwia Noga
  • Monika Michel
  • Bogusław BuszewskiEmail author


The objectives of this study were to investigate the molecular mechanisms of retention of selected fungicides and to classify stationary phases used for RP-HPLC. Chromatographic measurements were made using eight physicochemically diverse HPLC columns which were evaluated for analysis of nine biologically active compounds (strobilurins and triazoles). QSRRs were derived for the logarithms of the retention factors normalized to a hypothetical zero percent organic modifier in the mobile phase, log k W. Structural descriptors of the pesticides were used to describe their chromatographic retention behaviour under the given LC conditions. The next step was the correlation between retention data, log k W, and the structural descriptors of the analytes from molecular modelling.


Column liquid chromatography Chemically bonded stationary phases Retention mechanism QSRR Fungicides 



This work is supported by the European Social Fund, the Polish National Budget, and the Kujawsko-pomorskie Vovidship Budget—“Stypendia dla doktorantów 2008/2009-ZPORR”. Financial support from Foundation for Polish Science Professor’s Subsidy “Mistrz” is gratefully acknowledged.


  1. 1.
    Kaliszan R, Baczek T, Cimochowska A, Juszczyk P, Wisniewska K, Grzonka Z (2005) Proteomics 5:409–415CrossRefGoogle Scholar
  2. 2.
    Michel M, Baczek T, Studzinska S, Badzioch K, Jonsson T, Kaliszan R, Buszewski B (2007) J Chromatogr A 1175:49–54CrossRefGoogle Scholar
  3. 3.
    Kaliszan R (1992) Anal Chem 64:619A–631ACrossRefGoogle Scholar
  4. 4.
    Kaliszan R (2007) Chem Rev 107:3212–3246CrossRefGoogle Scholar
  5. 5.
    Romanelli GP, Autino JC, Castro EA (2002) Turk J Chem 26:335–343Google Scholar
  6. 6.
    Szepesy L (2002) J Chromatogr A 960:69–83CrossRefGoogle Scholar
  7. 7.
    Baczek T, Kaliszan R (2002) J Chromatogr A 962:41–55CrossRefGoogle Scholar
  8. 8.
    Jiskra J, Claessens HA, Cramers CA, Kaliszan R (2002) J Chromatogr A 977:193–206CrossRefGoogle Scholar
  9. 9.
    Baczek T, Kaliszan R, Novotna K, Jandera P (2005) J Chromatogr A 1075:109–115CrossRefGoogle Scholar
  10. 10.
    Ledesma EB, Wornat MJ (2000) Anal Chem 72:5437–5443CrossRefGoogle Scholar
  11. 11.
    Jakab A, Schubert G, Prodan M, Forgacs E (2002) J Chromatogr B 770:227–236CrossRefGoogle Scholar
  12. 12.
    Aschi M, D’Archivio AA, Maggi MA, Mazzeo P, Ruggieri F (2007) Anal Chim Acta 582:235–242CrossRefGoogle Scholar
  13. 13.
    Heberger K (2007) J Chromatogr A 1158:273–305CrossRefGoogle Scholar
  14. 14.
    Kaliszan R (1987) Quantitative structure-chromatographic retention relationships. Wiley, New YorkGoogle Scholar
  15. 15.
    Al-Haj MA, Kaliszan R, Nasal A (1999) Anal Chem 71:2976–2985CrossRefGoogle Scholar
  16. 16.
    Kaliszan R, van Straten MA, Markuszewski M, Cramers CA, Claessens HA (1999) J Chromatogr A 855:455–486CrossRefGoogle Scholar
  17. 17.
    Soczewinski E, Wachtmeister CA (1962) J Chromatogr A 7:311–320CrossRefGoogle Scholar
  18. 18.
    Valko K, Snyder LR, Glajch JL (1993) J Chromatogr A 656:501–520CrossRefGoogle Scholar
  19. 19.
    Snyder LR, Dolan JW (1998) Adv Chromatogr 38:115–185Google Scholar
  20. 20.
    Al-Haj MA, Kaliszan R, Buszewski B (2001) J Chromatogr Sci 39:29–38Google Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Sylwia Noga
    • 1
  • Monika Michel
    • 2
  • Bogusław Buszewski
    • 1
    Email author
  1. 1.Chair of Environmental Chemistry and Bioanalytics, Faculty of ChemistryNicolaus Copernicus UniversityToruńPoland
  2. 2.Plant Protection Institute, Pesticide Residue LaboratoryPoznanPoland

Personalised recommendations