, Volume 73, Supplement 1, pp 151–156 | Cite as

Synthesis and Evaluation of a Molecularly Imprinted Polymer for Pre-concentration of Patulin from Apple Juice

  • Afshin Rajabi KhorramiEmail author
  • Masoumeh Taherkhani
Full short Communication


Patulin, (4-hydroxy-4H-furo[3,2-c] pyran-2(6H)-one) is a toxic secondary metabolite produced by a wide range of fungal species growing on some fruits, including apples. A novel molecularly imprinted polymer (MIP) for patulin has been synthesized using oxindole as a dummy template. The synthesis of MIPs based on dummy templates is a solution to overcome “template bleeding” shortcoming in trace analysis. The polymer was prepared in a non-covalent approach with methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linker via free radical polymerization. It was revealed that the MIP particles in extraction columns displayed high affinity towards patulin and, therefore, subsequently employed for molecularly imprinted-solid phase extraction (MIP-SPE) of patulin from contaminated apple juice samples. The analysis of spiked samples showed good recoveries (>80%). Reproducibility, repeatability, and limit of detection of the proposed method were also investigated.


Column liquid chromatography Molecularly imprinted polymer Solid phase extraction Oxindole Patulin Apple juice 



This study was financially supported by the Islamic Azad University, Karaj Branch.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Islamic Azad University, Karaj BranchDepartment of ChemistryKarajIran
  2. 2.Islamic Azad University, Shahr-e-Ray BranchDepartment of ChemistryShahr-e-RayIran

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