, Volume 73, Issue 3–4, pp 367–371 | Cite as

Determination of Pyridinium Ionic Liquid Cations by Reversed Phase Ion-Pair Chromatography Using Gradient Elution

  • Lingmin Meng
  • Hong YuEmail author
  • Yuzhen Liu
Full Short Communication


Four pyridinium ionic liquid cations (N-ethyl-pyridinium, N-butyl-pyridinium, N-butyl-4-methyl-pyridinium, N-hexyl-pyridinium) were separated and determined by reversed phase ion-pair chromatography with ultraviolet-visible detection. The effects of ion-pair reagent, acetonitrile concentration and column temperature on the retention and separation of the cations were evaluated. Then the four pyridinium cations could be separated at baseline within 13 min. The detection limits (S/N = 3) were 0.30–0.70 mg L−1, and relative standard deviations (n = 5) for peak areas were 0.18–0.58%. The method was applied to analyze surface water with recoveries of 99.5–104.0%, which is accurate, reliable and practical.


Ion-pair chromatography Ionic liquids Gradient elution Ultraviolet-visible detection Pyridinium cations 



This work was supported by the Natural Science Foundation of Heilongjiang Province (grant no. B200909) and the Ministry of Education of Heilongjiang Province (grant no. 11541088).


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.College of Chemistry and Chemical EngineeringHarbin Normal UniversityHarbinPeople’s Republic of China

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