Identification of novel microsatellite loci in the sand martin, Riparia riparia, and cross-amplification of loci from other bird species
- 108 Downloads
We isolated and characterised six novel microsatellite loci for paternity analysis in the sand martin Riparia riparia, by screening an enriched genomic library. In addition, we tested 16 already published microsatellite markers, five of which were also polymorphic in the sand martin. Only one of these 11 loci exhibited evidence of null alleles, and all were polymorphic (mean H o = 0.68, range of number of alleles per locus = 4–24), making them suitable for individual heterozygosity quantification and paternity assessment in this species (exclusion probability of 11 unlinked loci = 0.999997).
KeywordsSand Martin Riparia riparia Paternity Microsatellite Cross-amplification
We thank Yoshan Moodley, Anna Grasse and Tomas Hrbek for technical advice during loci isolation and characterisation and Zsolt Nagy for assisting with field work. This research was funded by the Austrian Academy of Sciences and by OTKA grants (T042879, K69068) awarded to T.S. Blood samples were collected under permits issued by the Hortobágy National Park (22-66/2004).
- Griffith SC, Stewart IRK, Dawson DA, Owens IPF, Burke T (1999) Contrasting levels of extra-pair paternity in mainland and island populations of the house sparrow (Passer domesticus): is there an ‘island effect’? Biol J Linn Soc 68:303–316Google Scholar
- Hansson B, Bensch S, Hasselquist D, Lillandt B, Wennerberg L, von Schantz T (2000) Increase of genetic variation over time in a recently founded population of great reed warblers (Acrocephalus arundinaceus) revealed by microsatellites and DNA fingerprinting. Mol Ecol 9:1529–1538CrossRefPubMedGoogle Scholar
- Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
- Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
- Wagner RH (1998) Hidden leks: sexual selection and the clustering of avian territories. In: Parker PG, Burley N (eds) Avian reproductive tactics: female and male perspectives. Ornithological Monograph, vol 49. American Ornithologists’ Union, Washington, DC, pp 123–145Google Scholar