Characterization of polymorphic microsatellite loci from Round Island Petrels (Pterodroma arminjoniana) and their utility in other seabird species
Six microsatellite loci were isolated from the petrels of Round Island, near Mauritius in the Indian Ocean (Pterodroma arminjoniana). Three loci were monomorphic in P. arminjoniana but were found to be polymorphic in other Procellariiforms. Cross-utility of all six loci was tested in 17 Procellariiform and 1 penguin species. In addition, 53 microsatellite loci developed for other species of birds were tested for cross-species amplification in P. arminjoniana. Six of these loci were found to be polymorphic.
KeywordsPterodroma arminjoniana Round Island Petrel Trindade Petrel Procellariiform Microsatellite
Many thanks to Trent Garner at the Institute of Zoology for advice on laboratory protocols and Mark Blaxter and the NERC Molecular Genetics Facility at Edinburgh for assistance with sequencing. Thanks also to Joel Bried, Richard Phillips, Mareile Techow and Tom Hart for providing DNA samples. This work was supported by grants from the Natural Environment Research Council and the University of London Central Research Fund. The work described in this manuscript complies with current UK law.
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