Journal of Ornithology

, Volume 150, Issue 3, pp 703–707 | Cite as

Polymorphic microsatellites identified by cross-species amplifications in the European Coot Fulica atra

  • Adeline LoyauEmail author
  • Dirk S. Schmeller
Short Note


We tested the cross-amplification of 26 microsatellites developed for passerines and an additional three developed for Gallinula species in eight European Coots from two populations. Sixteen microsatellite markers successfully amplified, of which nine were polymorphic with 2–6 alleles (mean 3.7 alleles) and an expected heterozygosity (H e) ranging from 0.375 to 0.805 (mean H e = 0.589). On average, we found 2.22 alleles/locus and a mean H e of 0.440 in one nest, and 2.56 alleles/locus and a mean H e of 0.494 in the other one. These nine polymorphic markers could be of potential use in studies of genetic variability, population structure and reproductive strategy of European Coots.


Coot Fulica atra Microsatellite Cross-amplification 



Financial support by the Helmholtz Centre for Environmental Research-UFZ. M. Richard and the Laboratoire d’Ecologie kindly provided the 26 passerine primers and J.C. Buchan the sequences of the Gallinula primers. We also thank K. Henle, K. Schirmer, N. Stetefeld, W. Durka and S. Lampa.


  1. Buchan JC (2000) Behavioural and genetic aspects of mate-sharing in the Tasmanian native hen (Gallinula mortierii) and dusky moorhen (Gallinula tenebrosa). PhD thesis, the University of Queensland, Brisbane, AustraliaGoogle Scholar
  2. Lyon BE, Hochachka WM, Eadie JM (2002) Paternity-parasitism trade-offs: a model and test of host-parasite cooperation in an avian conspecific brood parasite. Evolution 56:1253–1266PubMedGoogle Scholar
  3. Otter K, Ratcliffe L, Michaud D, Boag PT (1998) Do female black-capped chickadees prefer high-ranking males as extra-pair partners? Behav Ecol Sociobiol 43:25–36CrossRefGoogle Scholar
  4. Primmer CR, Møller AP, Ellengren H (1996) A wide-range survey of cross-species microsatellite amplification in birds. Mol Ecol 5:365–378PubMedCrossRefGoogle Scholar
  5. Primmer CR, Painter JN, Koskinen MT, Palo JV, Merilä J (2005) Factors affecting avian cross-species microsatellite amplification. J Avian Biol 36:348–360CrossRefGoogle Scholar
  6. Van Hooft WF, Hanotte O, Wenink PW, Groen AF, Sugimoto Y, Prins HHT, Teale A (1999) Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer). Anim Genet 30:214–220PubMedCrossRefGoogle Scholar

Copyright information

© Dt. Ornithologen-Gesellschaft e.V. 2009

Authors and Affiliations

  1. 1.Department of Conservation BiologyHelmholtz Centre for Environmental Research-UFZLeipzigGermany
  2. 2.Station d’Ecologie Expérimentale du CNRS à MoulisSaint GironsFrance
  3. 3.Laboratoire d’EcologieUniversité Pierre et Marie Curie-Paris 6ParisFrance

Personalised recommendations