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Journal of Ornithology

, Volume 149, Issue 4, pp 495–506 | Cite as

Evidence from DNA nucleotide sequences and ISSR profiles indicates paraphyly in subspecies of the Southern Grey Shrike (Lanius meridionalis)

  • Javier Gonzalez
  • Michael Wink
  • Eduardo Garcia-del-Rey
  • Guillermo Delgado Castro
Original Article

Abstract

We obtained DNA sequence data from mitochondrial (cytochrome b) and nuclear genes (myoglobin and ornithine decarboxylase) to reconstruct the phylogenetic relationships among eight species of shrikes (Lanius). Phylogenetic analyses based on maximum parsimony, maximum likelihood and Bayesian inference all converged into a congruent topology and defined several well-supported clades. Our multi-gene approach based on nucleotide sequences from fast-evolving and conserved genes strongly supported the paraphyly of Southern Grey Shrikes (Lanius meridionalis). The Canary Islands subspecies (L. m. koenigi) differed significantly from its European counterpart (L. m. meridionalis). Furthermore, the genetic distinctiveness of L. m. koenigi was confirmed by ISSR genomic fingerprinting. By contrast, we did not find evidence to distinguish the Canarian Southern Grey Shrike from L. m. algeriensis on the African mainland (Tunisia), and therefore these two taxa may be considered as synonymous. Together, they correspond to a separate species. The origin of the taxa investigated in this study might have originated about 6 Mya at the Miocene/Pliocene boundary when a remarkable worldwide faunal turnover and global vegetation change occurred. The Lanius genus represents a complex and taxonomically challenging group that requires additional research.

Keywords

Cytochrome b Myoglobin Ornithine decarboxylase ISSR Phylogeny 

Notes

Acknowledgments

We are grateful to Gobierno de Canarias and the Cabildo Insular of Tenerife, Gran Canaria, Fuerteventura and Lanzarote for authorising the collection of biological samples which was partly funded by the Sociedad Ornitológica Canaria (SOC). We thank D. Concepción for collecting samples on Lanzarote. We thank P. Tryjanowski (Adam Mickiewicz University, Poland) and U. Olsson (Gothenburg University, Sweden) who provided samples of Lanius excubitor and L. meridionalis algeriensis, respectively. Thanks also to D. Ristow, G. Nikolaus, D. Guicking, T. van Wyk, H.-H. Witt, P. Heidrich and G.-M. Heinze for providing samples. We thank Prof. Dr. H. Bock (Managing Director of IWR) and S. Friedel for access to parallel computing facilities at the Interdisciplinary Center for Scientific Computing (IWR, Heidelberg University). We thank H. Sauer-Gürth who provided valuable technical assistance in our laboratory. M. Haase, C. Dietzen, T.C.H. Cole and Dr. M. Förschler provided valuable literature and comments to improve this manuscript. All experiments comply with the current laws of Germany.

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Copyright information

© Dt. Ornithologen-Gesellschaft e.V. 2008

Authors and Affiliations

  • Javier Gonzalez
    • 1
  • Michael Wink
    • 1
  • Eduardo Garcia-del-Rey
    • 2
  • Guillermo Delgado Castro
    • 3
  1. 1.Institut für Pharmazie und Molekulare BiotechnologieRuprecht-Karls-Universität HeidelbergHeidelbergGermany
  2. 2.Departamento de EcologíaFacultad de Biología, Universidad de La LagunaLa LagunaSpain
  3. 3.Museo de Ciencias NaturalesOrganismo Autónomo de Museos y CentrosSanta Cruz de TenerifeSpain

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