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In vivo 19F MR inflammation imaging after myocardial infarction in a large animal model at 3 T

  • Maik Rothe
  • Annika Jahn
  • Kilian Weiss
  • Jong-Hee Hwang
  • Julia Szendroedi
  • Malte Kelm
  • Jürgen Schrader
  • Michael Roden
  • Ulrich FlögelEmail author
  • Florian BönnerEmail author
Short Communication

Abstract

Objectives

Fluorine-19 (19F) MRI with intravenously applied perfluorocarbons allows the in vivo monitoring of infiltrating immune cells as demonstrated in small animal models at high field. Here, we aimed to transfer this approach to a clinical scanner for detection of inflammatory processes in the heart after acute myocardial infarction (AMI) in a large animal model.

Materials and methods

Optimization of coil and sequence performance was carried out on phantoms and in vivo at a 3 T Philips Achieva. AMI was induced in Munich mini pigs by 90-min occlusion of the left anterior descending artery. At day 3 after AMI, pigs received a body weight-adjusted intravenous dose of a perfluorooctyl bromide nanoemulsion followed by 1H/19F MRI at day 6 after AMI.

Results

A balanced steady-state free precession turbo gradient echo sequence using an ellipsoidal 19F/1H surface coil provided the best signal-to-noise ratio and a superior localization of 19F patterns in vivo. This approach allowed the reliable detection of 19F signals in the injured myocardium within less than 20 min. The 19F signal magnitude correlated significantly with the functional impairment after AMI.

Conclusion

This study demonstrates the feasibility of in vivo 19F MR inflammation imaging after AMI at 3 T within a clinically acceptable acquisition time.

Keywords

19F MRI Molecular imaging Inflammation Myocardial infarction Perfluorocarbons 

Notes

Acknowledgements

We thank Bernhard Schnackenburg, Jochen Keupp and Christian Stehning, Philips Research Hamburg; Martin Sager and Iris Schrey, Department of Animal Research and Animal Protection, Heinrich-Heine-University, Düsseldorf; Juliane Geisler and Mareike Gastl, Division of Cardiology, Pulmonology and Vascular Medicine, Heinrich-Heine-University, Düsseldorf for their excellent help and support. This study was supported in part by the German Federal Ministry of Health (BMG), by the Ministry of Science and Research of the State of North Rhine-Westphalia (MIWF NRW) and grants of the German Research Council (Deutsche Forschungsgemeinschaft, DFG; B0-4264/1–1, FL 303/6-1, SFB 1116).

Author contributions

Study conception and design: FB, UF, AJ, and MaR. Acquisition of data: FB, AJ, and MaR. Analysis and interpretation of data: MaR, AJ, FB, UF, and KW. Drafting of manuscript: MaR, FB, and UF. Critical revision: JHW, JS, MR, MK, JSz, and KW.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Copyright information

© European Society for Magnetic Resonance in Medicine and Biology (ESMRMB) 2018

Authors and Affiliations

  • Maik Rothe
    • 1
    • 2
  • Annika Jahn
    • 3
  • Kilian Weiss
    • 4
    • 5
  • Jong-Hee Hwang
    • 2
    • 7
  • Julia Szendroedi
    • 2
    • 6
    • 7
  • Malte Kelm
    • 3
    • 8
  • Jürgen Schrader
    • 1
  • Michael Roden
    • 2
    • 6
    • 7
  • Ulrich Flögel
    • 1
    • 3
    • 8
    Email author
  • Florian Bönner
    • 3
    Email author
  1. 1.Experimental Cardiovascular Imaging, Department of Molecular CardiologyHeinrich Heine UniversityDüsseldorfGermany
  2. 2.German Diabetes Center, Leibniz Center for Diabetes ResearchInstitute for Clinical DiabetologyDüsseldorfGermany
  3. 3.Division of Cardiology, Pulmonology and Vascular Medicine, Medical FacultyHeinrich Heine University, DüsseldorfDüsseldorfGermany
  4. 4.Philips ResearchHamburgGermany
  5. 5.Philips HealthcareHamburgGermany
  6. 6.Division of Endocrinology and DiabetologyHeinrich Heine UniversityDüsseldorfGermany
  7. 7.German Center for Diabetes ResearchMunichGermany
  8. 8.Medical Faculty, Cardiovascular Research Institute Düsseldorf (CARID)Heinrich Heine UniversityDüsseldorfGermany

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