Evidencing different neurochemical profiles between thalamic nuclei using high resolution 2D-PRESS semi-LASER 1H-MRSI at 7 T

  • Maxime Donadieu
  • Yann Le Fur
  • Sylviane Confort-Gouny
  • Arnaud Le Troter
  • Maxime Guye
  • Jean-Philippe Ranjeva
Research Article

Abstract

Objective

To demonstrate that high resolution 1H semi-LASER MRSI acquired at 7 T permits discrimination of metabolic patterns of different thalamic nuclei.

Materials and methods

Thirteen right-handed healthy volunteers were explored at 7 T using a high-resolution 2D-semi-LASER 1H-MRSI sequence to determine the relative levels of N-Acetyl Aspartate (NAA), choline (Cho) and creatine-phosphocreatine (Cr) in eight VOIs (volume <0.3 ml) centered on four different thalamic nuclei located on the Oxford thalamic connectivity atlas. Post-processing was done using the CSIAPO software. Chemical shift displacement of metabolites was evaluated on a phantom and correction factors were applied to in vivo data.

Results

The global assessment (ANOVA p < 0.05) of the neurochemical profiles (NAA, Cho and Cr levels) with thalamic nuclei and hemispheres as factors showed a significant global effect (F = 11.98, p < 0.0001), with significant effect of nucleus type (p < 0.0001) and hemisphere (p < 0.0001). Post hoc analyses showed differences in neurochemical profiles between the left and the right hemisphere (p < 0.05), and differences in neurochemical profiles between nuclei within each hemisphere (p < 0.05).

Conclusion

For the first time, using high resolution 2D-PRESS semi-LASER 1H-MRSI acquired at 7 T, we demonstrated that the neurochemical profiles were different between thalamic nuclei, and that these profiles were dependent on the brain hemisphere.

Keywords

Ultra high field 1H-MRSI Thalamic nuclei Neurochemical profiles Connectivity atlas 

Notes

Acknowledgments

The authors thank Dr. Malgorzata Marjanska from the CMRR (MI, USA) for the distribution of the semi-LASER sequence. We would like to thank Ben Ridley for useful suggestions on the manuscript. The first author is the recipient of a Ph.D. Grant (CIFRE) supported by Siemens France and the French ‘Association Nationale Recherche et Technologie’ (ANRT). This project is supported by the French IA Equipex 7T-AMI (2011) program ANR-11-EQPX-0001 and the A*MIDEX 7T-AMISTART (2013) through the ANR-11-IDEX-0001-02 program.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

10334_2016_556_MOESM1_ESM.tiff (154 kb)
B1 map obtained on the oil phantom 1 (TIFF 154 kb)
10334_2016_556_MOESM2_ESM.tiff (259 kb)
Corrected Cho/Cr ratios within homologous thalamic nuclei. * represents significative left/right neurochemical differences for each nucleus [Post-hoc Wilcoxon test (p < 0.05 corrected for multiple comparisons)] 2 (TIFF 258 kb)
10334_2016_556_MOESM3_ESM.docx (17 kb)
Supplementary material 3 (DOCX 16 kb)

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Copyright information

© ESMRMB 2016

Authors and Affiliations

  • Maxime Donadieu
    • 1
    • 2
    • 3
  • Yann Le Fur
    • 1
    • 2
  • Sylviane Confort-Gouny
    • 1
    • 2
  • Arnaud Le Troter
    • 1
    • 2
  • Maxime Guye
    • 1
    • 2
  • Jean-Philippe Ranjeva
    • 1
    • 2
  1. 1.Faculté de Médecine de la Timone, Centre de Résonance Magnétique Biologique et Médicale (CRMBM, UMR CNRS-AMU 7339), Medical School of MarseilleAix Marseille UniversitéMarseille Cedex 05France
  2. 2.AP-HM, CHU Timone, Pôle d’Imagerie, CEMEREMMarseilleFrance
  3. 3.Siemens HealthcareSt-DenisFrance

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