Evidencing different neurochemical profiles between thalamic nuclei using high resolution 2D-PRESS semi-LASER 1H-MRSI at 7 T
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To demonstrate that high resolution 1H semi-LASER MRSI acquired at 7 T permits discrimination of metabolic patterns of different thalamic nuclei.
Materials and methods
Thirteen right-handed healthy volunteers were explored at 7 T using a high-resolution 2D-semi-LASER 1H-MRSI sequence to determine the relative levels of N-Acetyl Aspartate (NAA), choline (Cho) and creatine-phosphocreatine (Cr) in eight VOIs (volume <0.3 ml) centered on four different thalamic nuclei located on the Oxford thalamic connectivity atlas. Post-processing was done using the CSIAPO software. Chemical shift displacement of metabolites was evaluated on a phantom and correction factors were applied to in vivo data.
The global assessment (ANOVA p < 0.05) of the neurochemical profiles (NAA, Cho and Cr levels) with thalamic nuclei and hemispheres as factors showed a significant global effect (F = 11.98, p < 0.0001), with significant effect of nucleus type (p < 0.0001) and hemisphere (p < 0.0001). Post hoc analyses showed differences in neurochemical profiles between the left and the right hemisphere (p < 0.05), and differences in neurochemical profiles between nuclei within each hemisphere (p < 0.05).
For the first time, using high resolution 2D-PRESS semi-LASER 1H-MRSI acquired at 7 T, we demonstrated that the neurochemical profiles were different between thalamic nuclei, and that these profiles were dependent on the brain hemisphere.
KeywordsUltra high field 1H-MRSI Thalamic nuclei Neurochemical profiles Connectivity atlas
The authors thank Dr. Malgorzata Marjanska from the CMRR (MI, USA) for the distribution of the semi-LASER sequence. We would like to thank Ben Ridley for useful suggestions on the manuscript. The first author is the recipient of a Ph.D. Grant (CIFRE) supported by Siemens France and the French ‘Association Nationale Recherche et Technologie’ (ANRT). This project is supported by the French IA Equipex 7T-AMI (2011) program ANR-11-EQPX-0001 and the A*MIDEX 7T-AMISTART (2013) through the ANR-11-IDEX-0001-02 program.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
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