Quantitative assessment of regional myocardial function in a rat model of myocardial infarction using tagged MRI

  • D. Thomas
  • V.A. Ferrari
  • M. Janik
  • D.H. Kim
  • S. Pickup
  • J.D. Glickson
  • R. Zhou
Research Article


We characterized global and regional left ventricular (LV) function during post myocardium infarction (MI) remodeling in rats, which has been incompletely described by previous MRI studies. To assess regional wall motion, four groups of infarcted animals corresponding to 1–2, 3–4, 6–8 and 9–12 weeks post-MI respectively were imaged using a fast gradient echo sequence with a 2D spatial modulation of magnetization (SPAMM) tagging preparation. An additional group was serially imaged (1–2 and 6–7 weeks post-MI) to assess the global function. Regional and global functional parameters of infarcted rats were compared to non-infarcted normal rats. Compared to normal rats, a decrease in ejection fraction (70 ±7 vs. 40 ± 8%, p<0.05) was observed in rats with MI. Maximal and minimal principal stretches (λ1, λ2) and strains (E1, E2), principal angle (β) and displacement varied regionally in normal rats but deviated significantly from the normal values in rats with MI particularly in the infarcted and adjacent zones. Not only was strain magnitude reduced segmentally post-MI, but strain direction became more circumferentially oriented, particularly in rats with larger infarctions. We report the first regional myocardial strain values in normal and infarcted rats. These results parallel findings in humans, and provide a unique tool to examine regional mechanical influences on the remodeling process.


Myocardial function Wall motion Tagged MRI Myocardial infarction Remodeling Rat 


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This study was supported by a grant from the National Institutes of Health (EB-002473) to RZ. We thank Dr. M. Brennick for usage of his MR volume coil.


  1. 1.
    American-Heart-Association Heart Disease and Stroke Statistics - (2003) UpdateGoogle Scholar
  2. 2.
    Gheorghiade M, Bonow RO (1998) Chronic heart failure in the United States: a manifestation of coronary artery disease. Circulation 97:282–289Google Scholar
  3. 3.
    Zhang S, Guo J, Zhang P, Liu Y, Jia Z, Ma K, Li W, Li L, Zhou C (2004) Long-term effects of bone marrow mononuclear cell transplantation on left ventricular function and remodeling in rats. Life Sci 74:2853–2864CrossRefPubMedGoogle Scholar
  4. 4.
    Pfeffer JM, Pfeffer MA, Braunwald E (1985) Influence of chronic captopril therapy on the infarcted left ventricle of the rat. Circ Res 57:84–95Google Scholar
  5. 5.
    Chandrashekhar Y, Sen S, Anway R, Shuros A, Anand I (2004) Long-term caspase inhibition ameliorates apoptosis, reduces myocardial troponin-I cleavage, protects left ventricular function, and attenuates remodeling in rats with myocardial infarction. J Am Coll Cardiol 43:295–301CrossRefPubMedGoogle Scholar
  6. 6.
    Nahrendorf M, Wiesmann F, Hiller KH, Hu K, Waller C, Ruff J, Lanz TE, Neubauer S, Haase A, Ertl G, Bauer WR (2001) Serial cine-magnetic resonance imaging of left ventricular remodeling after myocardial infarction in rats. J Magn Reson Imaging 14:547–555CrossRefPubMedGoogle Scholar
  7. 7.
    Saeed M, Bremerich J, Wendland MF, Wyttenbach R, Weinmann HJ, Higgins CB (1999) Reperfused myocardial infarction as seen with use of necrosis-specific versus standard extracellular MR contrast media in rats. Radiology 213:247–257PubMedGoogle Scholar
  8. 8.
    Schalla S, Wendland MF, Higgins CB, Ebert W, Saeed M (2004) Accentuation of high susceptibility of hypertrophied myocardium to ischemia: complementary assessment of Gadophrin-enhancement and left ventricular function with MRI. Magn Reson Med 51:552–558CrossRefPubMedGoogle Scholar
  9. 9.
    Nahrendorf M, Hiller K-H, Hu K, Ertl G, Haase A, Bauer WR (2003) Cardiac magnetic resonance imaging in small animal models of human heart failure. Med Image Anal 7:369–375CrossRefPubMedGoogle Scholar
  10. 10.
    Axel L, Dougherty L (1989) MR imaging of motion with spatial modulation of magnetization. Radiology 171:841–845PubMedGoogle Scholar
  11. 11.
    Axel L, Dougherty L (1989) Heart wall motion: improved method of spatial modulation of magnetization for MR imaging. Radiology 172:349–350PubMedGoogle Scholar
  12. 12.
    Rademakers F, Van de Werf F, Mortelmans L, Marchal G, Bogaert J (2003) Evolution of regional performance after an acute anterior myocardial infarction in humans using magnetic resonance tagging. J Physiol 546:777–787CrossRefPubMedGoogle Scholar
  13. 13.
    Epstein FH, Yang Z, Gilson WD, Berr SS, Kramer CM, French BA (2002) MR tagging early after myocardial infarction in mice demonstrates contractile dysfunction in adjacent and remote regions. Magn Reson Med 48:399–403CrossRefPubMedGoogle Scholar
  14. 14.
    Gotte MJ, van Rossum AC, Twisk JWR, Kuijer JPA, Marcus JT, Visser CA (2001) Quantification of regional contractile function after infarction: strain analysis superior to wall thickening analysis in discriminating infarct from remote myocardium. J Am Coll Cardiol 37:808–817CrossRefPubMedGoogle Scholar
  15. 15.
    Pfeffer JM, Pfeffer MA, Fletcher PJ, Braunwald E (1991) Progressive ventricular remodeling in rat with myocardial infarction. Am J Physiol 260:H1406–H1414PubMedGoogle Scholar
  16. 16.
    Zhou R, Pickup S, Glickson JD, Scott CH, Ferrari VA (2003) Assessment of global and regional myocardial function in the mouse using cine and tagged MRI. Magn Reson Med 49:760–764CrossRefPubMedGoogle Scholar
  17. 17.
    Nahrendorf M, Wiesmann F, Hiller KH, Han H, Hu K, Waller C, Ruff J, Haase A, Ertl G, Bauer WR (2000) In vivo assessment of cardiac remodeling after myocardial infarction in rats by cine-magnetic resonance imaging. J Cardiovasc Magn Reson 2:171–180PubMedGoogle Scholar
  18. 18.
    Axel L, Goncalves RC, Bloomgarden D (1992) Regional heart wall motion: two-dimensional analysis and functional imaging with MR imaging. Radiology 183:745–750PubMedGoogle Scholar
  19. 19.
    Kass M, Witkin A, Terzopoulos D (1988) Snakes: active contour models. Int J Comput Vis 1:321–331Google Scholar
  20. 20.
    Scott CH, Sutton MS, Gusani N, Fayad Z, Kraitchman D, Keane MG, Axel L, Ferrari VA (1999) Effect of dobutamine on regional left ventricular function measured by tagged magnetic resonance imaging in normal subjects. Am J Cardiol 83:412–417CrossRefPubMedGoogle Scholar
  21. 21.
    Young AA, Imai H, Chang CN, Axel L (1994) Two-dimensional left ventricular deformation during systole using magnetic resonance imaging with spatial modulation of magnetization. Circulation 89:740–752Google Scholar
  22. 22.
    Pfeffer MA, Pfeffer JM, Fishbein MC, Fletcher PJ, Spadaro J, Kloner RA, Braunwald E (1979) Myocardial infarct size and ventricular function in rats. Circ Res 44:503–512PubMedGoogle Scholar
  23. 23.
    Fletcher PJ, Pfeffer JM, Pfeffer MA, Braunwald E (1981) Left ventricular diastolic pressure-volume relations in rats with healed myocardial infarction. Effects on systolic function. Circ Res 49:618–626PubMedGoogle Scholar
  24. 24.
    Kramer CM, Rogers WJ, Theobald TM, Power TP, Petruolo S, Reichek N (1996) Remote noninfarcted region dysfunction soon after first anterior myocardial infarction. A magnetic resonance tagging study. Circulation 94:660–666Google Scholar
  25. 25.
    Kraitchman DL, Young AA, Bloomgarden DC, Fayad ZA, Dougherty L, Ferrari VA, Boston RC, Axel L (1998) Integrated MRI assessment of regional function and perfusion in canine myocardial infarction. Magn Reson Med 40:311–326PubMedGoogle Scholar

Copyright information

© ESMRMB 2004

Authors and Affiliations

  • D. Thomas
    • 1
  • V.A. Ferrari
    • 2
  • M. Janik
    • 2
  • D.H. Kim
    • 1
  • S. Pickup
    • 1
  • J.D. Glickson
    • 1
  • R. Zhou
    • 1
  1. 1.University of PennsylvaniaDepartment of RadiologyPhiladelphiaUSA
  2. 2.Cardiovascular DivisionSchool of Medicine, University of PennsylvaniaPhiladelphiaUSA

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