The experimental research on the inhibiting impacts of RNAi on Cyclin E in breast cancer cell line

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Abstract

Objective

The aim of the study was to investigate the biological character change of breast cancer cell line by inhibiting the Cyclin E expressing level.

Methods

Human breast cancer cell line MCF-7 was transfected by Cyclin E siRNA vector pEGFP/CCNE2. SiRNA-induced silencing of Cyclin E was determined at RNA level and protein level, respectively. The proliferation of MCF-7 and its sensitivity to chemical therapy were measured by CCK-8 assay, while cell cycle was measured by flow cytometry (FCM).

Results

The plasmid could reduce the expression of Cyclin E. The proliferation ability of MCF-7 was decreased and the sensitivity to chemical therapy was enhanced according to the inhibition of Cyclin E expression. The transfected MCF-7 was arrested at G1 phase of cell cycle.

Conclusion

The inhibition of Cyclin E can decrease the breast cancer cell’s growth, increase its sensation to chemical therapy and slow down its cell cycle. The Cyclin E siRNA may provide us a practical tool for further study on the gene therapy to breast cancers.

Key words

breast cancer Cyclin E RNAi 

Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  1. 1.Thyroid and Breast Surgery, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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