The Chinese-German Journal of Clinical Oncology

, Volume 6, Issue 5, pp 484–486

Establishment of nude mice model of human osteosarcoma cell MG63 with different potential of metastasis

Article

DOI: 10.1007/s10330-007-0068-6

Cite this article as:
Zhu, W., Guo, F., Xu, T. et al. Chinese German J Clin Oncol (2007) 6: 484. doi:10.1007/s10330-007-0068-6

Abstract

Objective

To establish a nude mice model of human osteosarcoma lung metastasis.

Methods

The growth of human osteosarcoma cell sublines M8 and M6 was determined by MTT assay. 2 × 107 cells were injected into the tail vein of nude mice. Mice were sacrificed started on week 4 after injection, and lung metastases were evaluated under both macroscopic and microscopic observation with HE staining.

Results

The growth of low-metastatic subline M6 was lower than high-metastatic sublines M8. Seventeen mice after injected M8 had occurred lung metastases while only one mice had occurred in M6 group. Moreover, M8 cells within metastases were arrangement disorder with variable nuclear hyperchromasia.

Conclusion

A mouse model for human osteosarcoma cancer lung metastasis can be established by injection different ability of metastasis MG63 cells into tail vein.

Key words

osteosarcoma potential lung, metastasis 

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of OrthopedicsTongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Rehabilitation MedicineTongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyWuhanChina

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