Apoptosis of human hepatoma cells induced by Gynostemma pentaphyllum Makino

  • Yuan Guohua 
  • Wei Jin 
  • Zhou Jingguo 
  • Guo Xiaolan 
  • Yang Minghui 
Article

Abstract

Abstract

Objective

To identify the proapoptotic effects of Gynostemma pentaphyllum Makino (GpM) on human hepatoma cells.

Methods

The effects of GpM on the cell apoptosis of human hepatoma cell line Huh-7 was assessed by flow cytomety. The expression of Bcl-2, Bcl-XL, Bax and Bad molecules in hepatoma cells treated with GpM was detected by Western blot.

Results

After treatment with 20 mg/mL GpM for 24 h, 56% of Huh-7 cells were undergoing apoptosis, while cell death was only observed in 12% of human fibroblast cells treated with GpM. Western blot demonstrated that, Bcl-2 was markedly decreased in Huh-7 cells treated with GpM. Whereas, Bax was significantly up-regulated in Huh-7 cells treated with GpM.

Conclusion

Treatment of human hepatoma cells with GpM induced apoptosis through the down-regulation of Bcl-2, and up-regulation of Bax.

Key words

Gynostemma pentaphyllum Makino apoptosis Bcl-2 Bax 

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Copyright information

© Editorial Office of the Chinese-German Journal of Clinical Oncology 2006

Authors and Affiliations

  • Yuan Guohua 
    • 1
  • Wei Jin 
    • 1
  • Zhou Jingguo 
    • 1
  • Guo Xiaolan 
    • 1
  • Yang Minghui 
    • 1
  1. 1.Institute of Rheumatology and ImmunologyAffiliated Hospital of North Sichuan Medical CollegeNanchongChina

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