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Simultaneous infection of sweet cherry with eight virus species including a new foveavirus

  • Hajime Yaegashi
  • Saki Oyamada
  • Shinichi Goto
  • Noriko Yamagishi
  • Masamichi Isogai
  • Tsutae Ito
  • Nobuyuki YoshikawaEmail author
Viral and Viroid Diseases
First Online:
  • 39 Downloads

Abstract

The agent causing bud blight disease (BBD) of sweet cherry (Prunus avium), first reported in Yamagata Prefecture, Japan, in the 1990s is graft-transmissible. Previously, little cherry virus 1 (LChV-1), little cherry virus-2 (LChV-2), cherry necrotic rusty mottle virus (CNRMV), cherry green ring mottle virus (CGRMV), and/or cherry virus A (CVA) were all detected from sweet cherry trees with BBD. Here, RNA viruses in sweet cherry trees with BBD were reinvestigated by high-throughput sequencing. Illumina RNA-sequencing of double-stranded RNAs from leaves of diseased cherry trees (S1, S2, S3, and S4) indicated that all trees were infected with at least six known viruses, LChV-1, LChV-2, CNRMV, CGRMV, CVA, prune dwarf virus (PDV), and/or apple chlorotic leaf spot virus. Thus, cherry trees with BBD are all infected with multiple viruses. Moreover, we found an undescribed novel virus of the family Betaflexiviridae (tentatively named cherry virus B, ChVB) in tree S3. The complete genome sequence of ChVB comprises 8806 nt, in which five open reading frames were found similar to the viruses of the genus Foveavirus. Homology search and phylogenetic analysis indicated that ChVB is a new species of the genus Foveavirus.

Keywords

Multiple virus infection Sweet cherry Bud blight disease Foveavirus High-throughput sequencing Cherry virus B 
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Notes

Acknowledgements

This study was supported by JSPS KAKENHI grant number 24380027.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals.

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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Apple Research, Institute of Fruit Tree and Tea ScienceNational Agriculture and Food Research Organization (NARO)MoriokaJapan
  2. 2.Faculty of AgricultureIwate UniversityMoriokaJapan
  3. 3.Horticultural Experiment StationYamagata Integrated Agricultural Research CenterSagaeJapan
  4. 4.Agri-Innovation CenterIwate UniversityMoriokaJapan

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